No 1 (2015)
Zh. A. Rupasova,
I. M. Garanovich,
T. V. Shpitalnaya,
T. I. Vasilyevskaya,
N. P. Krinitskaya,
L. V. Lyegkaya,
L. A. Murashkyevich
6-11 571
Abstract
Genotypic differences in biochemical composition of fruits such as natural form of black chokeberry and varieties Venissa and Nadzeja of Institute of Horticulture were established. It is shown, the selection process contributed to the impoverishment flavonols, organic free and phenol carbonic acids, pectin and tannins in berries, and their enrichment of antho-cyanins, catechins and soluble sugars (cultivar Venissa) and ascorbic acid (cultivar Nadzeja) with substantial improvement organoleptic properties.
12-15 468
Abstract
The morphogenesis of introduced species of blueberry high, cowberry, rhododendron yellow to various modifications of culture media, determine the optimal medium for the flow of the process were studied. The principal possibility of regeneration of introduced species of blueberry high cowberry by activation of axillary meristems, rhododendron yellow by two ways: 1) the activation of axillary meristems, 2) a proliferation of callus and the subsequent formation of his shoots are presented.
16-20 464
Abstract
The taxonomical structure, taxation parameters are studied and the assessment of a condition of wood plantings of the territory of the park complex «Mir» which is subject to reconstruction (regular park) was given.
21-25 602
Abstract
The characteristics of damage, desiccation conifers and hardwoods in extreme violations of forest ecosystems (periodic manifestation of extreme climate and weather conditions, the intensification of sanitary and intermediate felling was studied. Together, this leads to a decrease in efficiency of growing forests, the emergence of economic damage as a result of partial loss of merchantable timber and the need for early logging, measures for its recovery. The expediency of cutting damaged trees without further growth prospects before they dry up. Criteria developed by eye early diagnosis of deterioration of trees in terms of damage to foliage, crown and trunk, available skilled forestry production in everyday work. The improved method and technological aspects of selective sanitary cuttings in compliance with modern approaches, environmental management requirements under extreme and normal periodic fluctuations in climate and weather conditions were proposed.
26-29 653
Abstract
The data about rizogeneze and morphometric parameters of the regenerants of dioskorea (Dioscorea alata L.), which are developed on the modified ion-exchange substratum TRIONA®, with the use of a new class of soil meliorant - the hydro gels of different composition, capable of absorbing and of retaining in the swollen state an enormous quantity of moisture with the dissolved in it nutrients are represented. It is shown that the action 1 mg/l of the hydro gel of large or small fraction ECOFLOC A-07 (Chinese Peoples' Republic) without the supplemental application of fertilizers is optimum for accelerating the development of the regenerants of dioskorea on the optimized ion-exchange substratum TRIONA®.
30-33 524
Abstract
Belarusian isolate of Blackcurrant reversion virus (BRV) was characterized on molecular level during the study. The high level of conservation of 3'non-translated region of RNA-2 was revealed for BRV genome. Belarusian isolate was closely related to BRV isolate from Finland. Phylogenetic analysis showed that clustering of investigated isolates didn't depend on their geographical origin or plat-hosts.
34-37 522
Abstract
In this paper, as a possible mechanism of architectural reorganization of chromatin matrix are considered properties of Arg-X proteolysis in due to the physiology of growth and development of the embryo-seedling. Perhaps hypersensitivity in the trypsin complexes to Arg-X activity of core blocks of chromatin strongly bound with nuclear matrix entrenched at transforming of winter variety from original spring.
38-42 576
Abstract
It was found that the most promising raw materials of domestic origin for the isolation of hypericin is the herb St. John's worth. An effective scheme of purification of Hypericum perforatum extracts, allowing in optimal conditions to obtain drugs with hypericin content of not less than 5 wt.% , was proposed.
43-49 499
Abstract
As the result of the study of microsatellite DNA loci polymorphism in the population of diploid interspecific hybrids originated from wild allotetraploid potato species S. stoloniferum, two SSR-loci were revealed presence of that was correlated with high level of late blight resistance. The analysis of obtained data made it possible to conclude that the SSR-locus STM3023-200 (mapped on chromosome IV) may be considered as PCR-marker of late blight resistance gene of S. stoloniferum (not mapped earlier) which is related to Rpi-genes of R2 family. The locus STM0031-173 may be used as the PCR-marker for detection of genetic locus, probably being the analog of QTL VII/P49, mapped on chromosome VII in several segregating diploid populations of hybrids with late blight resistance genes introgressed from wild potato species.
D. V. Sauchyn,
T. N. Veresova,
O. A. Mezhnina,
A. S. Panush,
A. O. Vyacheslavova,
I. V. Goldenkova-Pavlova
50-55 570
Abstract
The native gox gene encoding glucose oxidase (GOX) was isolated from the genomic DNA of Penicillium funiculosum. For high-level expression of the P. funiculosum GOX in S. tuberosum, low-usage codons were replaced by high-usage ones. The optimized gene was synthesized and cloned under the control of a CaMV 35S promoter into the plant transformation vector. Potato plants were transformed via an Agrobacterium tumefaciens-mediated procedure by selecting for kanamicyn resistance. Stable integration and expression of the optimized gox gene in the transgenic potato lines were ascertained by molecular and biochemical tests. Using codon optimization technique, the expression level of the native P. funiculosum gox gene in S. tuberosum plants was improved 20 %.
56-59 476
Abstract
A set of unified linearized vectors to improve the solubility of the target protein by its fusion with different partner proteins (glutathione-S-transferase, and disulfide isomerase of Escherichia coli) was constructer for the first time in this work. The kit may be used in studies to promote solubility of the target proteins by designing chimeric protein structures.
60-68 504
Abstract
Multistage adaptation of bacteria Arthrobacter sulfonivorans BIM В-2242 to lactose resulted in selection of morphologically and biochemically stable strain Arthrobacter sulfonivorans LF-GAL exceeding 1.5 times the parent strain in efficiency of extracellular β-galactosidase synthesis (22.8 U/ml vs 15.2 U/ml). Optimal composition of nutrient medium was formulated for submerged fermentation of strain A. sulfonivorans LF-GAL to secure rise in enzyme productivity up to 35 U/ml, which is 1.5 times higher than its initial level and 2.3 times superior to that of the parent strain. Obtained results will be used for developing bacterial β-galactosidase biotechnology.
69-72 446
Abstract
The results of studies of the reproductive system of male rats obtained from irradiated at a dose of 0,5 Gy females and intact males are presents. It is shown that in experimental animals revealed a number of deviations of the studied parameters of the reproductive system, which are treated as long-term consequences irradiated female.
73-77 527
Abstract
Forest fires in the contaminated areas may increase the concentration of radioactive substances in the air close to the source as well as at large distances. The model of the atmospheric transport of radioactive aerosols and their loss due to dry deposition is presents in the article. The density of contamination of radioactive fallout over long distances (5-10 km) is comparable to the background values in the paper.
78-81 492
Abstract
The electronic microscopy study of the erythrocytes and platelets ultrastructure in rats with arterial hypertension, diabetes mellitus and comorbidity diseases are showed. It has been estimated that diabetes mellitus accelerates the development of atherosclerosis and it is the morphological basis of cerebrovascular complications.
82-88 618
Abstract
The etiology, biological properties and sensitivity of the causers of wound infections to traditionally used antimicrobial remedies has been study. For prevention of the further shaping antimicrobial resistance is confirmed need of the undertaking time- and doso- depending killing of causers, allowing provide the rational choice of the remedy and forecast the frequency of the bandagings with it. High efficiency of the undertaking decontamination of air is shown by means of recirculator, equiped by mercury without ozone lamps with output of ray < 200 nm, for warning exogenous infection of wounds. The practicability of the use of blighty herbal raw materials for development and introduction of import substitution innovation remedies efficient in respect of as planktonic, so and biofilm of the forms of the causers was proved.
89-94 504
Abstract
Narrow clawed crayfish juvenile experiences 7-9 molts during its first year period. The number of molts is gradually reduced in subsequent years: at the age of 6 it is two molts for male and one for female. Percentage molt increment is characterized by high dispersion. Narrow clawed crayfish youth increase their linear size on 11,3 %, mature individuals - on average 8,19 ± 3,27 % during one molt.
95-99 500
Abstract
The data on the characteristics of the calanoid copepoda E. velox life cycle in the water bodies of Belarus were obtained. The presence of at least two generations (summer and winter) in the annual growth of the crustacean was established. Breeding period E. velox is extended and passes continuously during the summer-autumn period. It is observed 4 density peaks of the absolute number in almost all stages of development.
100-105 478
Abstract
The minimum, average and maximum sizes of the body of allogenic and indigenous amphipods types for the reservoirs of Belarus have been specified in the work. The shown parameters are similar for Belarus and for Europe and are a little higher than sizes in the area of amphipod origin. It can be determined by the influence of different environment factors in new habitats.
106-108 469
Abstract
At the first the results on the extensity and intensity of telaziosis in cows and buffalos in Iraq were given. Registered pathogens - Thelazia gulosa, T. skrjabini, T. rhodesi. It was determined the dynamic of infection telazies cows and buffaloes in the 3 provinces of Iraq. In the province of Najaf was registered the highest growth of infection cows telazies which begins two months earlier, reaching a peak in November. In Buffalo was identified one type telazies - Thelazia gulosa. It was found that an increase in the infestation of buffalo of telazies surveyed provinces of Iraq begins later than in cows.
109-112 552
Abstract
The results of the study of the biological characteristics of the virus VHSV in connection with a huge potential threat of entry of the virus into specialized fish farms of Ukraine. Determined by the pathogenicity of these isolates to rainbow trout fingerlings (Oncorhynchus mykiss). It is established that the studied isolates of the virus of hemorrhagic septicemia of trout had different pathogenicity in relation to rainbow trout fingerlings, and caused the death of 35 to 65 %. Studied the reproduction of these isolates in cell lines FHM, RTG-2.
REVIEWS
113-120 528
Abstract
Potato leaf roll virus, a widespread harmful pathogen with low genetic variability of the population, is transmitted by aphids (persistently). The main types of plant resistance to this pathogen are: resistant to infection, virus accumulation and movement. Identified sources valuable for breeding, combining different types of resistance, controlling their resistance genes mapped, developed molecular markers suitable for use in marker - assisted selection.
SCIENTISTS OF BELARUS
121-123 417
ISSN 1029-8940 (Print)
ISSN 2524-230X (Online)
ISSN 2524-230X (Online)