Quantitative content of the sum of phenolic compounds and anthocyanins in their composition were study. The level of antiradical activity (ARA) in the berries and leaves of Vaccinium corymbosum L. was measured.

The maximum content of the sum of phenolic compounds was observed in Blueray, Bluerose, Caroline Blue, Herbert, Jersey, Nelson cultivars. In the cultivars which have been studied the quantity of anthocyanins in the composition of phenolic compounds ranged from 0.01 to 0.8 % in green berries, and from 22 to 52 % in mature ones. Earlyblue, Duke, Bluecrop, Bluegold cultivars were characterized by the greatest quantity of phenolic compounds in leaves.

The presence of a close positive correlation between the level of antiradical activity and the content of secondary metabolites of phenolic nature was determined. The differences in phenolic substances accumulation in blueberry cultivars obtained by in vivo and in vitro methods were revealed.

It was shown that the proteomic profile of the highbush blueberry plants together with genetic passports can being used to determine the cultivar's identity of Vaccinium corymbosum L.

This work evaluates the test results of 3 types of LED lamps with different spectral composition of radiation and photon flux of photosynthesis (PPF, μmol/m²/sec): PPG T8I Agro (37), Navigator DSP-FITO (47.5) and DDP06-3×8-004 UHL 4 ‘Home Farm' (100), in the greenhouse culture of the orchid Cattleya labiata Lindl. The parameters of the thickness of annual leaves, the percentage of dry matter and the increase in biomass per unit of leaf surface (μg/cm²) formed over a period of 140 days were used as indicators to assess the degree of influence of the spectral composition of light on the morphogenesis of C. labiata plants. The spectral characteristics of the LED lamp DDP06-3×8-004 UHL 4 ‘Home Farm' with a white glow, intensity PPF 100 are considered as the most favorable for the successful greenhouse cultivation of the orchid Cattleya labiata Lindl. The combination in its spectrum of red and green spectra with their ratio equal to 1.3, and when the ratio of red and blue spectra equal to 3.34, a higher level of dry matter accumulation is observed per unit of leaf surface area white maintaining parameter of the leaf structure characteristic of a given plant species.

The results of a comparative study of the influence of mineral and organic fertilizers - N₁₆P₁₆K₁₆, Nanoplant, Hydrohumate, and Ecosil - on the state of the antioxidant complex of early-ripening (Northcountry, Croaton) and mid-ripening (Bluecrop, Northland) varieties of tall blueberries in an experimental culture on a low-lying peat bog in the Central agroclimatic zone of Belarus are presented. A significant dependence of changes in the level of their antioxidant and enzymatic activity on the type of fertilizers and the genotype of plants is shown. In most varieties, the increase in mineral nutrition contributed to an increase in the antioxidant activity level up to 28 %, the most significant when using Nanoplant and Hydrogumate. Revealed a distinct antagonism in changing the activity of peroxidase and catalase, on the one hand, and polyphenol oxidase, on the other: it increases synchronously in the first pair of enzymes to 77 and 30 % in varieties Northcountry, 171 and 152 % in the variety Croaton, 137 and 182 % in the variety Bluecrop and to 122 % of varieties for the Northland, against a preferential decrease in the activity of polyphenol oxidase in 13-55 %.

In most cases, the tested agricultural practices have contributed to raising the general level oxidase activity of blueberry fruit. Especially in Croaton and Bluecrop varieties, by 30-310 %, with the highest efficiency of organic fertilizers, especially Hydrohumate. Nanoplant treatments had an inhibitory effect on the general level oxidase activity in Northcountry and Northland varieties and a stimulating effect in Croaton and Bluecrop varieties.

The study of wound-healing effect of the gel substances with 2 and 4 % (w/w) concentrations of the dry extracts of the everlasting flowers and of the littlewale leaves in the ratio 1:1 were carried out.

It was found that the skin application of the studied gel substances promoted the formation of the structural and functional regenerate in the area of the wound defect. The studied gel contributed to the significant reduction of the healing duration of the full-thickness linear wounds in rats compared to the control group of animals (without treatment) and to the group of animals that received treatment with the reference listed drug. The use of the studied gels, regardless of the content of the plant extracts in them (2 or 4 % (w/w)), was not accompanied by toxic effects.

Rhododendrons are some of the most attractive flowering shrubs in the landscape. The article presents the results of studying the common problems of Rhododendron collection in the Central Botanical Garden of the National Academy of Sciences of Belarus, conducted in 2012-2020. There are several diseases and pests that commonly occur on rhododendrons every year. These include powdery mildew (Erysiphe azaleae), fungal leaf spots (Pestalotiopsis rhododendri, Colletotrichum gloeosporioides, Alternaria spp., Sphaerulina azaleae, Phyllosticta concentrica, Phyllosticta rhododendricola), black vine weevil (Otiorhynchus sulcatus), garden foliage beetle (Phyllopertha horticola), click beetle (Hemicrepidius niger), red spider mite (Tetranychus urticae), physiological leaf spots, oedema, winter injury, chlorosis. Less common on rhododendrons are stem diseases (Botryosphaeria dothidea, Cadophora spp., Hymenoscyphus spp.), wilt (Fusarium oxysporum), bud blight (Seifertia azaleae), leaf and flower gall (Exobasidium rhododendri), rhododendron lacebug (Stephanitis rhododendri), greenhouse whitefly (Trialeurodes vaporariorum). There were identified that all evergreen and half evergreen rhododendrons have some resistance to powdery mildew, deciduous rhododendrons are more resistant to fungal leaf spots than evergreens. Damage to rhododendrons by insect pests usually does not cause significant harm but heavy feeding results in an unsightly plants.

315 variants of halotolerant bacteria were isolated from soil samples taken around Starobin potash deposit mined by Belaruskali concern. Nitrogen-fixing isolate CA-6 and phosphate-solubilizing isolate Cp-1 capable to withstand abiotic stress caused by sodium chloride in concentration 1710 and 2565 mM, respectively and water deficiency characterized by osmotic potential -0.20 and -0.42 MPa were sorted out. Isolate Cp-1 may dissolve phosphates yielding halo zones 9 mm in diameter while isolate CA-6 displays nitrogenase activity 44.0 nM C₂H₂/vial/3 days. Both isolates are growth-promoting organisms, distinguished by the ability to synthesize IAA in concentrations 89.7 and 37.8 μg IAA/ml of cultural liquid, respectively. The selected salt-resistant isolates were identified based on analysis of the nucleotide sequence of the 16S rRNA gene and data of MALDI-TOF mass spectrometry and were referred to the species Rhodococcus jostii and Priestia megaterium. Halotolerant strains Priestia megaterium Cp-1 and Rhodococcus jostii CA-6 were deposited in Belarusian collection of non-pathogenic microorganisms under the respective registration numbers BIM B-1314D and BIM B-1353D.

The parameters of growth and development of the microalga Haematococcus pluvialis, as well as the process of carotenogenesis, including the pigment composition and expression of the key genes of astaxanthin biosynthesis, were studied in algal cells under the combined action of excess of NaCl in the culture medium and high light intensity. It was found that the combination of excess of NaCl in the culture medium for Haematococcus pluvialis, initiated at the resting stage of the microalga, and high light intensity is not an effective method for induction of the accumulation of astaxanthin in the cells of Haematococcus pluvialis. A decrease in the level of expression of one of the key genes of carotenogenesis - phytoene synthase gene, was revealed under such conditions. At the same time, it was found that the combined action of high light intensity and excess of NaCl led to an increase in the ratio of astaxanthin cis-isomers, which are more biotechnologically valuable, in the total pigment pool.

The population of noble crayfish (Astacus astacus) was researched in quarries of an old brick plant in Molodechno district of Minsk region. Rare disappearing noble crayfish was found in 10 closed quarries. The presence of crayfish in closed quarries that are not connected with watercourses indicates that these populations were created by man. The population of noble crayfish in the quarries of an old brick factory is characterized by a high number. The average catch by eel traps was 1.31 ± 0.77 individuals/trap/day. Crayfish in the first and second year of life grew faster in quarries than in other habitats, but subsequently the average size of individuals in the quarry was smaller in comparison with the general model of noble crayfish growth in Belarus. Individuals that were older than 6 years have not been found in quarries. This indicates that there are factors that determine high mortality rate in older age groups. However, favorable conditions for the growth of juveniles allow the population to maintain their numbers at a high level. The reason for this phenomenon remains unknown. The size of noble crayfish individuals reaching puberty and the fertility is comparable to those from other habitats. In the quarry, the minimum length of an oviparous female was 7.2 cm. Egg-laying females in the 7.0-7.9 cm size class were 16.7 %, in the 8.0-8.9 cm size class - 38.8, in the 9.0-9.9 cm size class - 70.6 %, in the size class of 10.0-10.9 cm all the females were oviparous.

The population of noble crayfish from the quarries of Molodechno district should be considered as donor population for carrying out resettlement into the water bodies of the river Neman.

The article describes the structure and seasonal dynamics of spider species diversity in bilberry and mossy pine forest in the republican reserve “Pribuzhskoe Polesie”. 51 species had been registered in the bilberry pine forest and 52 species in the mossy pine forest. In total, 71 species which belong to 17 families had been registered. 53 new species for the territory of the reserve and one new species for Belarus are indicated. Studies have shown that the species diversity of spiders has a similar structure, which is confirmed by the results of calculating the Shannon, Pielu, Simpson and Margalef indices. In the bilberry pine forest, the number of individuals and the dynamic density are higher, while in the mossy pine forest there is evenness and species richness. The dominant species in the mossy pine forest is Trochosa terricola (relative abundance 25.0 %), in the bilberry pine forest there are two dominants - Alopecosa aculeata and T. terricola (30.3 and 23.5 %, respectively).

Proteomic analysis is a highly effective method for bacteria identification and the elucidation of protein's content in prokaryotic cells at different growth conditions. To our knowledge this approach is hardly ever used for characterization of producers of biologically active substances. The understanding of the changes in protein profile in mutant strains capable of biologically active substances overproduction helps to recognize the biochemical and molecular basis of metabolic changes which lead to overproduction. So that, proteomic analysis could be especially useful for optimization the producer's creation techniques.

The purpose of current research was to carry out proteomic profiling of bacteria P. chlororaphis subsp. aurantiaca mutant strains capable of overproduction of phenazine antibiotics. Microbiological and biochemical methods were used for these aims.

In current research a proteomic analysis of strains of P. chlororaphis subsp. aurantiaca producing phenazines was carried out. An early (during log-phase) onset of expression of individual genes of phz-operon which codes enzymes for phenazines synthesis was demonstrated. It was also found that the wild type strain has the highest level of PhzO protein. The gene encoding this protein is located outside the phz-operon. We weren't able to establish the correlation among PhzO protein content and concentration of the derivatives for which appearance PhzO is responsible. A general tendency of producer strains towards the accumulation of enzymes and proteins of the antioxidant defense system was revealed. Producer strains also demonstrate a significant increase in the concentration of proteins involved in DNA repair as well as chaperones involved in the native protein conformation maintenance.

Screening of sporulating bacterial strains of genus Bacillus isolated from various natural sources was carried out. The most active antagonistic bacteria were chosen. Probiotic properties of the selected spore-farming Bacillus were investigated: spectrum of antimicrobial action, safety for macroorganism, survival in transit via gastrointestinal tract (bile and acid resistance), lack of inhibiting effect on gut microbiota, native non-plasmid resistance to widely applied antimicrobial agents. The prospects of using the most active cultures as key components of probiotics and feed additives for farm stock, poultry and fish were demonstrated.

This review is devoted to one of the actual problem of modern biology and medicine, we are witnessing. The text provides information on 3D printing method, equipment that is used to manufacture of three-dimensional bioproducts and the nature of the consumable materials - bioinks, fillers and cells which during printing transform into three-dimensional bioproduct characterized by vitality and expression of specific marker factors of corresponding tissues and organs. Special emphasis in this review is made on prospects of use of stem cells which are along with a variety of cells of tissues and organs have found application in bioprinting. Three methods of bioprinting: inkjet, extrusion and laser are described. Advantages and disadvantages of each method are discussed. Bioinks, their properties applicability to various printing technologies and strategy selection of bioink and cell composition defining viability of three-dimensional structures and possibility of their application in clinical praxis are detailly described. Short list of achievements in the field of bioprinting of various organism tissues and the characteristics of the bioproducts obtained are given. Significant progress and promising research in the field of 3D bioprinting are marked.