Analysis of the group of double daffodils was done. Features of seasonal development were distinguished. By using a five-point scale assessed the decorative qualities of 34 introducents. Defined reproductive ability of breeds and their degree of resistance to disease. On set of decorative and bioutility parameters the 12 varieties are recommended for urban greening. These varieties differ in color and morphological structure of the flower. They are relatively stable in the local environment to biotic and abiotic environmental factors.

The results of research carried out under the State program of socio-economic development and integrated management of natural resources of Pripyat Polesie for 2010-2015 allowed to estimate natural resource potential of natural grassland and meadow communities in the region. Currently, as it was observed, there are negative changes of natural grasslands in all administrative districts Pripyat Polesie which associated with a reduction of the rural population, the spread of shrubs, the forming bumps. Separate grassland arrays differ from little kontur and waterlogging. It is required to normalize the current situation to set specific measures for each agricultural organization in Polesie region based on the correct information on the natural features and the state of grasslands.

nanoparticles lead to cell death, induce oxidative stress and reactive oxygen species formation, causing dose-dependent generation of ROS, but also induce DNA damage in a concentration of 50-100 pg/ml. ROS and subsequent oxidative stress are the most likely causes of the negative consequences and ultimately the cell death by apoptosis.

Exogenous 5-aminolevulinic acid (ALA) stimulated the respiration and photosynthesis in cotyledons of winter rape plants. Sulfonylurea herbicide Magnum did not reduce activity of respiration or even stimulated the process that was accompanied with similar effects on the activity of cytochrome ooxidase. Herbicide significantly inhibited photosynthetic activity, changed the structure of pigment-protein complexes of the reaction centers of Photosystem (PS) 2, light-harvesting complexes of PS1 and PS2 through decreasing content of their protein components. ALA in the presence of Magnum stimulated photosynthesis and respiration if herbicide inhibited these processes but decreased rate of them in case of their stimulation by herbicide. It was concluded that in the presence of herbicide ALA supported optimal homeostasis of respiration and photosynthetic as well as activity of cytochrome c-oxidase.

The expression of AOX1 and ANT genes encoding ADP/ATP-transporting protein and cyanide-resistant oxidase respectively in seedlings of different winter wheat varieties under low temperature stress is studied. It is shown that under normal conditions the expression levels of genes encoding ADP/ATP-antiporter (ANT) and mitochondrial cyanide-resistant oxidase is higher in winter wheat varieties more tolerant to low temperature stress. With the help of the «level of ANT and AOX1 gene expression» parameter promising accessions of winter wheat with increased resistance to the low temperatures were revealed, which indicates the prospects of usage of this parameter in the selection process of winter wheat.

oxygen affinity.

Strain Rhodococcus jialingiae HCT-91 isolated from natural sources is capable to utilize trimethylamine as a sole source of carbon and nitrogen. In batch culture it degrades toxicant in concentration 100 mg/l by 6 hours. Hypothetical pathway of trimethylamine catabolism by strain Rh. jialingiae HCT-91 was proposed. It was demonstrated that trimethylamine is oxidized to trimethylamine-N-oxide further split up to dimethylamine and acetaldehyde. Dimethylamine is degraded to methylamine and the latter is cleaved into acetaldehyde and ammonium. Acetaldehyde is broken down to carbon dioxide and water. As a result cells of strain Rh. jialingiae HCT-91 carry out complete trimethylamine mineralization.

Molecular marker analysis of winter wheat varieties, entered in the State register of the Republic of Belarus, with the help of has shown the presence of the gene Lr1 in cultivars Sanata, Usdym, Jadvisja, Elegia, Sakret, Akteur, Finezja, Dоrota, Muza, Turnia. The leaf rust resistance gene Lr10 was identified in the genome of winter varieties Finezja and Dorota. The functional allele Lr34+ was revealed in cultivars Fantazija, Dar Zelenograda, Don 93 and Akteur. The gene Lr37 was identified in varieties Sailor and Skagen. Not loci linked to the resistance genes Lr9, Lr19, Lr20, Lr21, Lr22a, Lr24, Lr25, Lr28, Lr29, Lr35, Lr42 and Lr47 were identified in the investigated varieties.

Species composition of macrozoobenthos for 58 stations of 44 transboundary Belarus-Russia-Ukraine rivers was determined. It is shown that the values of the Extended Biotic Index has high values and correspond to the first and second class of water quality. The results of hydrobiological studies allow us to conclude the absence of environmental ecological risks for studied aquatic ecosystems. Studied transboundary rivers are not channels of cross-border transport of pollutants. Transboundary river network is the buffer system and ensures a high ecological quality of surface waters in the region.

The paper contains a list of 15 species of Lumbricidae, including 2 species Octolaswn cyaneum (Savigny, 1826) and Aporrectodea ictericus (Savigny, 1826), which have not been noticed before in the territory of Belarus.

Based on the study of subfossil material from excavations of archaeological monument Gomel 1988 detinets (castle) (XII-XIII cc.) the species composition of fishes is given (23 species). The age of 15 species of fish is determined. It is found that the main commercial species for the inhabitants of the castle were Stizostedion lucioperca L. (63.1 % of total subfossil remains), Abramis brama (L.) (7.5 %) and Esox lucius L. (6.1 %). The anadromous fish - Rutilus frisii (Nord.) - currently in Belarus do not occur, and Vimba vimba (L.) submitted not anadromous form. Fish catch XII-XIII centuries are based on the older age groups Stizostedion lucioperca (L.) - 8-9 years. Unlike the ancient catches, modern fish catches from p. Sosh are based mainly on the younger age groups of fish Stizostedion lucioperca (L.) - 2, 3 years.

Apple trees of different cultivars growing in Belarus were tested using RT-PCR for Apple stem grooving virus, an important economically and common pathogens in commercial orchards. 115 apple trees and 64 raws of clonal rootstocks were tested. 9.6 % of apple trees were infected with ASGV. In 6.2 % of clonal rootstock were foud infected plants. Specificity of molecular markers was confirmed by sequencing the RT-PCR product. The nucleotide and deduced amino acid sequences were compared with sequences published in GenBank. Alignment this nucleotide and amino acid sequences of hypothetical protein sequence with other similar sequences from GenBank showed up to 99 and 100 % identity, respectively.

The results of ApMV diagnostics efficiency with using frozen leaves as the tested material were presented in the article. It was determined that after leaves storage at -20 °C optical density did not differ significantly from using of fresh material. Conservation of antigenic activity didn’t depend on host plant cultivar. The estimation of ELISA-test susceptibility using the range of cell sap dilution showed that using the plant sample with high concentration of virus made possible to detect ApMV in highly diluted solution (up to 1:1000). Storage of plant extracts from frozen leaves after homogenization influenced negatively on viral capacity to react with cover antibodies.

It was found that multiplex RT-PCR (mRT-PCR) method with two combinations of primers (1F/1R, MF05-22-F/MF05-21-R, AtropaNad2.1a/2.2b and RDP-1/RDP-2, MF05-22-F/MF05-21-R, AtropaNad2.1a/2.2b) can be used for the simultaneous and differential detection of blueberry pathogens (Blueberry scorch carlavirus, Tobacco ringspot nepovirus). Application of mRT-PCR for detection of virus diseases of blueberry plants allows to reduce testing costs on 49.5 % in comparison with simplex RT-PCR.

The results of cytophysiological studies of HEK293 cells treated with plant alkaloids are present. It is shown that these natural compounds possess broad range of biological activity having both anti- and oxidative properties. Texture analysis method was implemented to quantitatively assess mitochondrion structure changes after the treatment. The results suggest a strong correlation between morphology and distribution of mitochondria in cell cytoplasm with changes in membrane potential level of these organelles during oxidative stress.

There was elaborated a new quantitative method for evaluating the antifungal properties of the agents, which are based on a determination of dehydrogenase activity of filamentous fungi. Comparative quantitative parameter (DA) was proposed to reduce measurement errors that were obtained under intermediate precision. There was established a high correlation degree of test results of antifungal properties of drugs based on PHMG obtained with the new and standard methods.

The object of genetic researching have been family groups of pedunculate (Quercus robur L.) and sessile (Quercuspetraea (Matt.)) oaks of different origin. It was found that the level of genetic variation and subdivision in the hybrid families was lower compared to the families of parental species. Genotypic analysis of the half-sibling offspring showed asymmetric nature of the interspecific hybridization processes of pedunculate and sessile oaks in mixed stands.

Diphyllobothriosis of hamsters is going with genotoxic effect in somatic cells of host which characterized by growth number of single cell breaks, alkali-labile sites nucleus molecule DNA of bone marrow cells till 7.1 %. Underskin three-times sensibilization by PSP from D. latum tissues in dose of 800 mcg/g is characterized by genotoxic and cytotoxic effects in somatic cells of bone marrow and generative cells of testicals of CBA line mice which appears by growth of number of single cell breaks, alkali-labile sites nucleus molecule DNA to 2.41-7.16 % and apoptotic cells to 2.40-4.60 %. Invasion by dyphyllobothriosis is going with oxidative stress in hepatic cells and testicals of host, which characterized by growth of concentration of products POL (MDA, DK) lower levels of ferments-antioxidates activity (SOD, catalase) common antioxydative activity and correlates with growth of cytogenetic damages of bone marrow cells.

is equal to 37 ± 12 ^M. Oxidative processes depends on cell metabolic activity and induces cell morphological impairments: cell detachment from the substrate, cell dissociations and swelling, which reflects necrotic mechanism of oxidative cell death.

PVM and PVS are widespread and harmful pathogens, in their populations isolated strains. Viruses spread mechanically, infected tubers and insects. There are genes, which control the extreme and hypersensitive resistance to these viruses. Effective molecular markers developed to these genes for use in breeding. Varieties created with genes of hypersensitivity to M-and S-viruses and breeding material with extreme resistance gene to PVM.