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Proceedings of the National Academy of Sciences of Belarus, Biological Series

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No 3 (2014)
5-11 503
Abstract
on the biochemical composition of the root on the sod-podzolic sandy loam soil are presented. It is shown a weak influence on the agronomic testing the content of carbohydrates, especially soluble sugars, and a highly significant accumulation of phenolic compounds, especially leucoanthocyanins. Most effective in terms of improving the quality of medicinal raw valerian root biochemical composition proved separate use of copper and zinc micronu-trients, as well as the combined use of boron and zinc micronutrients, while the least efficient - separate use micronutrients boron and combined application of boron and copper, as well as copper and zinc micronutrients.
12-14 694
Abstract
This paper presents data on the regenerative capacity of different types of explants from introduced species of rhododendrons in a sterile culture. It is shown that the apex of the germ has the greatest potential for regeneration, which gives the maximum yield of regenerated plants per explant. This allowed it to be recommended as the primary explant for micropropagation studied species of rhododendrons.
15-18 477
Abstract
In the first high bioactivity efficiency of three forms of growth regulator : Ecosil plus VE ; Ekogum , VE ; Ekogum AF , VE has been experimentally proven under on gerbera plants .
19-22 535
Abstract
This research is devoted to examining of Fusarium (wide spread pathogen's) proteases. As far as pathogen's proteases are supposed to be one of infection agent they are released into surrounding medium and their synthesis is induced by different factors which were established. Spectrum of alkalic and neutral proteases activities are studied as well as inhibitory activity toward trypsin both in culture medium and mycelium. It was shown that inhibitors derived from mycelium and plant inhibitors effectively suppressed pathogen's protease activity. Research results contribute to disclosure of mechanism.
23-30 442
Abstract
Results of the comparative analysis of variability of 8 quantitative traits at regenerants of Northland and Patriot cultivars of highbush blueberry in vitro on nutrient, agarized mediums for rooting, with organic compounds, on macro- and micro- salt basis of / WPM differing on composition of auxins in 9 variants of experience are given presented. Distinctions on variability of analyzed traits depending on phytohormonal structure of the medium are established. A certain regularity of variability of height and crude weight of regenerants with growth of concentration of applied phytohormones was observed. Usage of phytohormones in comparison with control more often led to reliable increase in indicators of regenerant height by 1.21.4 times, lengths of the third interstice by 1.2-1.3 times (only at Northland cultivar), the crude weight of regenerants by 1.21.4 times (only at Northland cultivar), viability of explants by 1.2-1.4 times (only at Patriot cultivar). It is established reliable (in most cases at P <0.01) influence of a genotype, phytohormonal structure of the medium and set of two factors on variability of the vast majority of analyzed traits, respectively - seven of eight traits from shares of influence of 3-30 %, all traits from shares of influence of 19-56 %, six of eight traits from shares of influence of 10-42 %.
31-37 478
Abstract
The collection consisted of 15 cultivars of foreign breeding and 23 cultivars of Belarussian breeding, included in the State register of varieties, trees and shrubs, approved for use in the production in the Republic of Belarus, was analyzed with use of 16 microsatellite markers. The studied cultivars were revealed to be less polymorphic as compared with those cultivated in other European regions. It was found that the cultivars grown in Belarus, were the closest to cultivars from the Central Europe according to the level of polymorphism. The set of wheat microsatellite markers required for DNA certification of cultivars grown in Belarus was suggested. This set can be used for identification of cultivated and checking of new cultivars for novelty and also, if it is necessary, for elucidation of the degree of relationship between the different varieties.
38-41 575
Abstract
The action of carbon dioxide on electron transport of photosystem 2 was shown to be similar to that of inhibitor of phosphorylation sodium fluoride, which prevents migration of light harvesting complexes from photosystem 2 to photosystem 1.
42-45 448
Abstract
The localization and dynamics of an antimicrobial protein thionin in green and etiolated barley seedlings cv Honar was studied. It was shown that the content of thionin in etiolated barley seedlings is much higher than that registered in green plants. In the bottom of the green leaf the content of thionin greatly exceeds that of the upper part of the sheet. It was found that only in the bottom part of the leaf thionin content increases with age of the seedlings. When placed in a dark, green seedlings show increasing thionin content but it does not reach the amount registered in the etiolated leaves of the same age. It was assumed that the high level of thionin in the lower part of seedlings and increase of it in darkness is consistent with the general strategy of plant defence against pathogens and caused by the fact that the nature of the lower part of the plant is contacted with the soil (located closer to the ground), where due to the presence of pathogenic micro-organisms and their spores as well as high humidity, especially at night, chances of infection are high.
46-51 473
Abstract
It was shown that potato plants treated with ALA (1 mg / L) under normal growth conditions have higher antioxidant capacity compared with control plants due to higher content of glutathione, water-soluble phenols and anthocyanins. Under low temperature stress (-4 ± 1 °C, for 1 h) in ALA treated plants APX, GH are activated, glutathione pools, water-soluble phenols and anthocyanins are increased, significantly exceeding these values in ALA untreated plants, that is consistent with our previous results that showed lower level of lipid peroxidation products and the low membrane permeability in ALA treated plants under stress. Thus, the results indicated that application of exogenous ALA improves potato tolerance to low temperature.
52-56 781
Abstract
It was shown that it is possibility to optimize growing conditions of chlorella using culture medium on the basis of complex fertilizers Kristalon, which is cheaper and simpler in terms of process, as well as modifying the lighting conditions of algae, which will reduce the cost of its production.
57-61 528
Abstract
M) was investigated. Ethyl salicylate more than twice increased total SA content in leaves, moreover, seeds soaking in this solution led to seedling growth inhibition and significant increase ROS content in leaves. Exogenous CK, 3,5-dinitrosalicylic acid and phenyl salicylate increased endogenous pool of salicylates in moderate level (20 to 34 %). The possibility of using SA and phenyl salicylate as inductors of systemic resistance in barley plants through increasing endogenous SA is discussed.
62-65 523
Abstract
(0.5-5.5 mM) into the nutrient medium before inoculation resulted in reduced biomass accumulation by 39-86.6 % and decreased level of enzyme generation - 19-83.8 %. Inhibitory impact of oxidation stress on growth of P. varians BIM F 563 D and glucose oxidase biosynthesis at stationary phase of fungal culture was demonstrated. Resistance of P. varians BIM F 563 D to oxidation stress may be promoted by treatment inoculum with non-lethal doses of stress factor. Phenomenon of cross tolerance was established in P. varians BIM F 563 D: preliminary exposure of seed material to temperature and ethanolic stress raised fungal resistance to oxidation stress.
66-69 602
Abstract
Stable dispersion form of oleic acid triglycerides in conjunction with polyhexamethylene guanidine hydrochloride were obtained. These forms have strong bactericide properties in concentration of 0.04 % and above (of active material): ensure reduction of test bacteria cells number in five degrees or more. Thus respiratory activity of obligate-aerobic bacteria is reduced more than nine times.
70-73 739
Abstract
It was measured systemic changes in expression levels of pathogenesis related genes in Nicotiana benthamiana plants infected by Pectobacterium carotovorum. Rnary to the situation in infected leaves, we have observed no induction of PR genes in systemic tissues. Moreover, expression of four PR genes was significantly decreased. Virus induced silencing of the SIPK and WIPK genes in N. benthamiana has shown that these genes are required for the observed suppression effect.
74-79 473
Abstract
The cicadas trophic groups identification was set. The oligophages scale is proposed to be set strictly according to taxo-nomic affiliation of forage plants. Polyphages' groups are also ranked according to particular life form of a forage plant. In accordance to the proposed approach all 417 cicadas' species registered in Belarus are classified among trophic groups. The predominance of oligophages which comprise 4/5 of all registered in Belarus species is shown.
80-88 748
Abstract
Lifespan of narrow-clawed crayfish individuals in Somenskoe Lake is 6 years, the individuals reach 13 cm length by the end of this period. Theoretical maximum lifespan of crayfish individuals is around 9 years. The difference between the theoretical maximum lifespan and the current lifespan of narrow-clawed crayfish in the lake is due to intense amateur fishing. Female fecundity varies from 100 to 260 eggs. The average diameter of recently laid egg is 2,96 ± 0,16 mm, dry weight -4,54 ± 0,62 mg . Dry egg weight is 29,7 ± 3,7 % of raw. There is no statistical dependency between the mass of a single egg and female individual mass . Fecundity at the end of the embryonic period is less than the number of eggs in their early laying by 7-10 %. All females become mature at the age of 3. Habitat of Sominskoe lake can be characterized as favorable for narrow-clawed crayfish populations.
89-93 683
Abstract
The summer phenological group of leaf mining insects damaging green stands of Brest Polesie (Belarus) includes Trachys minutus L. (Buprestidae), Isochnus sequensi Stierlin (Curculionidae), Heterarthrus ochropoda (Klug, 1818), Profenusapyg-maea Klug, 1816 (Tenthredinidae), Phyllonorycter roboris Zell., Phyllocnistis unipunctella Steph. (Gracillariidae), Eriocrania sparrmannella Bosc (Eriocraniidae), Stigmella speciosa Frey, Stigmella lapponica Wocke (Nepticulidae), Lyonetia prunifo-liella Hübn., Leucoptera malifoliella Costa (Lyonetiidae). Among them are monophagous (3 species), oligophagous (5 species) and polyphagous (3 species). Under condition of the region the most common species are E. sparrmannella, Ph. roboris and Ph. unipunctella.
94-100 531
Abstract
Pu in biological chain «soil - ration - organs and tissues». translocation and accumulation coefficients, characterizing the movement of radionuclides from soil to organs and tissues were calculated.
101-104 525
Abstract
The data of the helminth fauna of Norway rat in Belarus are presented. A total in little animals were found 8 species of helminths: 1 Trematoda species, 3 Cestoda species and 4 Nematoda species. 20 Norway rats caught on meliorative canal banks, in landscape reserve «Bugskiy» and along of motor-roads (Brest, Zhabinka and Malorita districts of Brest region; south-west part of Belarus) and investigated during 1996-2011. 6 species of helminths (1 trematode, 3 cestodes, 2 nematodes) were found in 12 little animals. Mesocercariae of trematode Alaria alata (Goeze, 1782) have been registered more often.

REVIEWS

105-115 548
Abstract
Quinones induce reactive oxygen species production and interact with SH-groups of biological molecules resulting in the change of cellular redox state, proteinkinases, phosphatases and transcription factors activity. The efficiency of quinone impact on cell functioning depends on the intracellular state parameters, structure pequliarities and physicochemical properties of these compounds. The recent data about molecular mechanisms of cellular signaling pathways regulation by quinone has been reviewed.
116-120 554
Abstract
Two species of genus Thyreus bees were registered on the territory of Belarus: T. truncatus (Pérez, 1883) and T. histri-onicus (Illiger, 1806). Finding of species T. orbatus (Lepeletier, 1841) recorded on the Latvian territory seems to be possible. We suppose that T. truncatus (Pérez, 1883) has a wider range of hosts than it is currently known.

SCIENTISTS OF BELARUS



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ISSN 1029-8940 (Print)
ISSN 2524-230X (Online)