Scrining of the glucoamylase-producing strains of Aspergillus awamori
Abstract
The scrining of Aspergillus awamori strains with increased production of amylolytic enzymes was performed. The potato starch cleavage capability of micromycetes was evaluated based on the amount of reducing sugars generated and by the glucose oxidase/peroxidase assay. Using native and SDS-PAAG electrophoresis of extracellular proteins and zymographic technique the presence of amylolytic enzymes presumably related to glucoamylase and a-amylase with molecular weights of 114, 63 and 51 kDa was detected.
About the Authors
O. B. Rus
Белорусский государственный университет
Belarus
Belarus
A. V. Kachan
Белорусский государственный университет
Belarus
Belarus
A. N. Evtushenkov
Белорусский государственный университет
Belarus
Belarus
References
1. Miller G. L. // Anal. Chem. 1959. Vol. 31. P. 426-429.
2. Laemmli U. K. // Nature. 1970. Vol. 227. P. 680-685.
3. Koc O., Metin K. // African Journal of Biotechnology. 2010. Vol. 9, № 23. P. 341-342.
4. Upadhyay M. K., Sharma R., Pandey A. K, Rajak R. C. // Mycologist. 2005. Vol. 19, № 4. Р. 138-140.
5. Evstatieva Y., Nikolova D, Ilieva S. et al. II Second Balkan conference on biology, 21-23 may 2010, Plovdiv, 50 years University of Plovdiv. Plovdiv 2010. P. 613-617.
6. Dubey A. K., Suresh C., Kavitha R. et al. II FEBS Letters. 2000. Vol. 471. P. 251-255.
7. Yamasaki Y., Suzuki Y., Ozawa J. // Agric. Biol. Chem. 1977. Vol. 41. P. 2149-2155.
8. Negi S., Banerjee R. // Biotechnol. Bioprocess Engineering. 2009. Vol. 14. P. 60-66.
9. Ali S., Hossain Z., Mahmood S. Alam R. // World J. Microbiol. Biotechnol. 1990. Vol. 6. P. 431-433.
10. Vandersall A.S., Cameron R. G., Nairn C.J. et al. // Prep. Biochem. 1995. Vol. 25. P. 29-55.
11. Selvakumar P., Ashakumary L., Helen A., Pandey A. // Lett. Appl. Microbiol. 1996. Vol. 23. P. 403-406.
12. Xiao Z., Storms R., Tsang A. // Analytical Biochemistry. 2006. Vol. 351, № 1. P. 146-148.
Review
Views: 511
Email this article 
