MOLECULAR GENETIC ANALYSIS OF THE DETERMINANTS ASSOCIATED OF ANTIMICROBIAL PROPERTIES OF BACTERIA PSEUDOMONAS BRASSICAсEARUM BIM B-446
Abstract
As a result of transposon mutagenesis of bacteria Pseudomonas brassicacearum BIM B-446, more than 100 mutants were selected and characterized with different antimicrobial activity, the synthesis of fluorescent pigments and mobility, comparing with original strain. Molecular genetic analysis of four mutants revealed that changing’s in studied properties were caused by inserting a mini-Tn5 transposon in the regulatory genes (transcription factors), that determinate regulation of the general metabolism of the bacterial cell.
About the Authors
A. A. MURATOVA
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Belarus
M. N. MANDRYK-LITVINKOVICH
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Belarus
T. L. NASONOVA
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Belarus
L. N. VALENTOVICH
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Belarus
E. I. KOLOMIETS
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Belarus
M. A. TITOK
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Belarus
References
1. Pseudomonas genomes: diverse and adaptable / M. W. Silby [et al.] // FEMS Microbiol Rev. FEMS Microbiol Rev. – 2011. – Vol. 35, N 4. – Р. 652.
2. Population dynamics of non-pathogenic Fusarium and fluorescent Pseudomonas strains in rockwool, a substratum for soilless culture / A. Epavier [et al.] // FEMS Microbiol. Ecol. – 1991. – Vol. 86. – P. 177–184.
3. Effect of Pseudomonas chlororaphis on Pythium aphanidermatum and root rot in peppers grown in small-scale hydroponics troughs / A. Khan [et al.] // Biocontrol Sci. Technol. – 2003. – Vol. 13. – P. 615–630.
4. Timing Pseudomonas chlororaphis applications to control Pythium aphanidermatum, Pythium dissotocum, and root rot in hydroponic peppers / S. Chatterton [et al.] // Biol. Control. – 2004. – Vol. 30. – P. 360–373.
5. Mini Tn5 transposon derivatives for insertion mutagenesis, promoter probing- and chromosomal insertion of cloned DNA in Gram-negative Eubacteria / V. de Lorenzo [et al.] // J. Bacteriol. – 1990. – Vol. 172. – P. 6568–6572.
6. Сэги, Й. Методы почвенной микробиологии / Й. Сэги. – М.: Колос, 1983. – С. 253.
7. King, E. O. Two simple media for the demonstration of pyocyanin and fluorescein / E. O. King, M. K. Ward, D. E. Raney // J. Lab. Clin. Med. – 1954. – Vol. 44. – P. 301–307.
8. Te Riele, H. Single-stranded plasmid DNA in Bacillus subtilis and Staphylococcus aureus / H. te Riele, B. Michel, S. D. Ehrlich // Proc. Natl. Acad. Sci. USA. – 1986. – Vol. 83, N 8. – Р. 2541–2545.
9. Sambrook, J. Molecular Cloning: A Laboratory Manual. / J. Sambrook, E. Fritsch, T. Maniatis // Cold Spring Harbor Publications. – N. Y., 1989. – P. 468.
Review
Views: 560
Email this article 
