One-pot enzymatic synthesis of deoxynucleoside trihosphates
Abstract
Using methods of genetic engineering strains E. coli producing deoxynucleoside kinase of D. melanogaster, deoxynucle-oside monophosphate kinase of bacteriophage T5, homologous nucleoside-diphosphate kinase and acetate kinase, with additional С-terminal fusion polyhistidine oligopeptide were constructed. Four canonical deoxynucleoside triphosphates were synthesized using the recombinant enzymes. The yields of the reactions were 95-97 %. The conversion was completed within 4 h.
About the Authors
A. N. Rymko
Институт микробиологии НАН Беларуси
Belarus
Belarus
S. V. Kvach
Институт микробиологии НАН Беларуси
Belarus
Belarus
A. I. Zinchenko
Институт микробиологии НАН Беларуси
Belarus
Belarus
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