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Proceedings of the National Academy of Sciences of Belarus, Biological Series

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One-pot enzymatic synthesis of deoxynucleoside trihosphates

Abstract

Using methods of genetic engineering strains E. coli producing deoxynucleoside kinase of D. melanogaster, deoxynucle-oside monophosphate kinase of bacteriophage T5, homologous nucleoside-diphosphate kinase and acetate kinase, with additional С-terminal fusion polyhistidine oligopeptide were constructed. Four canonical deoxynucleoside triphosphates were synthesized using the recombinant enzymes. The yields of the reactions were 95-97 %. The conversion was completed within 4 h.
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ISSN 1029-8940 (Print)
ISSN 2524-230X (Online)