Morphological changes in the hepatopancreas of freshwater mollusks exposed to aliphatic alcohols with different carbon chain lengths
https://doi.org/10.29235/1029-8940-2026-71-4-344-352
Abstract
Aliphatic alcohols are among the factors of anthropogenic pollution of aquatic ecosystems. Structural changes in the hepatopancreas of pulmonate freshwater mollusks under their exposure remain insufficiently studied. In this work, a comparative assessment of morphological changes in the hepatopancreas of the ramshorn snail (Planorbarius corneus L.) and the great pond snail (Lymnaea stagnalis L.) was performed following acute exposure to aliphatic alcohols with different carbon chain lengths (C1–C6).
The experiment involved a one-hour exposure to solutions of methanol, ethanol, isopropanol, butanol-1, and hexanol-1, followed by a 48-hour recovery period. The degree of morphological changes in the hepatopancreas was found to depend on the concentration and type of alcohol. The most pronounced changes were observed following exposure to isopropanol, butanol-1, and hexanol-1. The ramshorn snail was more resistant to alcohol exposure and secreted more mucus, whereas in the great pond snail ethanol stimulated reproductive activity.
The obtained results confirm the high sensitivity of the hepatopancreas of freshwater mollusks to aliphatic alcohols. This justifies the use of morphological changes in the hepatopancreas as a biomarker for the ecotoxicological monitoring of aquatic ecosystems.
Keywords
About the Authors
M. V. VishnevskayaBelarus
Maria V. Vishnevskaya – Postgraduate Student
33, Moskovskiy Ave., 210038, Vitebsk
P. Yu. Pinchuk
Belarus
Polina Yu. Pinchuk – Postgraduate Student
33, Moskovskiy Ave., 210038, Vitebsk
A. A. Chirkin
Belarus
Alexander A. Chirkin – D. Sc. (Biol.), Professor, Professor of the Department
33, Moskovskiy Ave., 210038, Vitebsk
References
1. Sidorov A. V. Neuromodulatory effects of hydrogen peroxide on central neurons in the feeding network of the mollusk Lymnaea stagnalis. Journal of Evolutionary Biochemistry and Physiology, 2017, vol. 53, no. 6, pp. 493–500. https://doi.org/10.1134/S0022093017060060
2. Sidorov A. V. Model’nye organizmy i kletochnaya organizatsiya nervnykh tsentrov. Nauka i innovatsii [Science and Innovations], 2018, no. 6, pp. 10–14 (in Russian).
3. Golubev A. P. The dynamics of radioadaptation processes in mollusk populations in water bodies within the Belarusian sector of Chernobyl nuclear contamination zone. Ekologicheskii vestnik [Ecological Bulletin], 2012, no. 2, pp. 44–57 (in Russian).
4. Khomich A. S., Axenov-Gribanov D. V., Bodilovskaya O. A., Shirokova Y. A., Shchapova E. P., Lubyaga Y. A., Shatilina Z. M., Emshanova V. A., Golubev A. P. Assessment of the joint effect of thermal stress, pollution, and parasitic infestation on the activity of antioxidative enzymes in pulmonate mollusk Lymnaea stagnalis. Contemporary Problems of Ecology, 2017, vol. 10, no. 2, pp. 157–163. https://doi.org/10.1134/S1995425517020068
5. Dromashko S. E., Shevtsova S. N., Babenko A. S. Influence of heavy metals on the large pond snail Lymnaea stagnalis L. Minsk, Belaruskaya Navuka Publ., 2018. 172 p. (in Russian).
6. Chirkin A. A., Balaeva-Tikhomirova O. M. Molekulyarno-strukturnaya gomologiya proteoliticheskikh fermentov: monografiya. Cheboksary, Publishing house “Sreda”, 2022. 124 p. (in Russian).
7. Rőszer T. The invertebrate midintestinal gland (“hepatopancreas”) is an evolutionary forerunner in the integration of immunity and metabolism. Cell and Tissue Research, 2014, vol. 358, no. 3, pp. 685–695. https://doi.org/10.1007/s00441-014-1985-7
8. Klimova Y. S., Chuiko G. M., Pesnya D. S., Ivanova E. S. Biomarkers of oxidative stress in freshwater bivalve mollusks (review). Inland Water Biology, 2020, vol. 13, no. 4, pp. 674–683. https://doi.org/10.1134/S1995082920060073
9. Wu H., Yan R., Li A., Huang D., Pan J., Zhao P., Xing Z. Efficient treatment of alcohol wastewater and its potential pollutant control strategies. Journal of Water Process Engineering, 2024, vol. 58, art. 104882. https://doi.org/10.1016/j.jwpe.2024.104882
10. Salikhyanova S. A. Ochistka stochnykh vod na predpriyatii spirtovoy promyshlennosti. Vestnik magistratury [Bulletin of the Magistracy], 2019, no. 3–2, pp. 51–52 (in Russian).
11. Lushchak V. I. Environmentally induced oxidative stress in aquatic animals. Aquatic Toxicology, 2011, vol. 101, no. 1, pp. 13–30. https://doi.org/10.1016/j.aquatox.2010.10.006
12. Sharma P., Chukwuka A., Chatterjee S., Chakraborty D., Saha N. C. Pathological and ultrastructural changes of Bellamya bengalensis under chronic carboxylic acid exposure at environmentally relevant levels: inferences from GUTS predictions and hepatopancreatic integrity assessment. Chemosphere, 2024, vol. 361, art. 142542. https://doi.org/10.1016/j.chemosphere.2024.142542
13. Strubelt O., Deters M., Pentz R., Siegers C. P., Younes M. The toxic and metabolic effects of 23 aliphatic alcohols in the isolated perfused rat liver. Toxicological Sciences, 1999, vol. 49, no. 1, pp. 133–142. https://doi.org/10.1093/toxsci/49.1.133
14. Ingólfsson H. I., Andersen O. S. Alcohol’s effects on lipid bilayer properties. Biophysical Journal, 2011, vol. 101, no. 4, pp. 847–855. https://doi.org/10.1016/j.bpj.2011.07.013
15. , Oliveira-Filho E. C., Grisolia C. K., Paumgartten F. J. Effects of endosulfan and ethanol on the reproduction of the snail Biomphalaria tenagophila: a multigeneration study. Chemosphere, 2009, vol. 75, no. 3, pp. 398–404. https://doi.org/10.1016/j.chemosphere.2008.11.085
16. Wu D., Cederbaum A. I. Alcohol, oxidative stress, and free radical damage. Alcohol Research and Health, 2003, vol. 27, no. 4, pp. 277–284.
17. Zhang Z., Yang H., Han F., Guo P. Reactive oxygen species as key molecules in the pathogenesis of alcoholic fatty liver disease and nonalcoholic fatty liver disease: future perspectives. Current Issues in Molecular Biology, 2025, vol. 47, no. 6, art. 464. https://doi.org/10.3390/cimb47060464
18. Kaviraj A., Bhunia F., Saha N. C. Toxicity of methanol to fish, crustacean, oligochaete worm, and aquatic ecosystem. International Journal of Toxicology, 2004, vol. 23, no. 1, pp. 55–63. https://doi.org/10.1080/10915810490265469
19. Dymond M. K. A membrane biophysics perspective on the mechanism of alcohol toxicity. Chemical Research in Toxicology, 2023, vol. 36, no. 6, pp. 793–804. https://doi.org/10.1021/acs.chemrestox.3c00039
20. Baltz D. M., Chesney E. J., Tarr M. A., Kolok A. S., Bradley M. J. Toxicity and Sublethal Effects of Methanol on Swimming Performance of Juvenile Florida Pompano. Transactions of the American Fisheries Society, 2005, vol. 134, no. 3, pp. 730–740. https://doi.org/10.1577/T04-136.1
Review
JATS XML



























