Carotenogenesis in Dunaliella salina cells IBCE D-1 strain during cultivation on modified media under conditions of different illumination
https://doi.org/10.29235/1029-8940-2025-70-4-331-340
Abstract
The combined effect of nutrient deficiency in the cultivation medium of Dunaliella salina IBCE D-1 strain and high-intensity light is more effective than the effect of nutrient deficiency alone in inducing the accumulation of carotenoids in algae cells. At the same time, nitrogen deficiency in the nutrient medium leads to greater production of β-carotene by cells than potassium and phosphorus deficiency. A predominant suppression of photosystem 2 (PS2) activity was observed in D. salina cells cultured on a nitrogen-deficient medium was established in comparison with to the control and variant with potassium and phosphorus deficiency. This may be a key factor in triggering increased synthesis of β-carotene in algae cells, as an antioxidant that prevents excessive accumulation of reactive oxygen species in chloroplasts, in particular, singlet molecular oxygen, the generation of which increases when components are damaged or the functioning of PS2 complexes is disrupted.
About the Authors
T. V. Samovich
V.F. Kuprevich Institute of Experimental Botany of the National Academy of Sciences of Belarus
Belarus
Belarus
Tatsiana V. Samovich – Ph. D. (Biol.), Leading Researcher, V.F. Kuprevich Institute of Experimental Botany of the National Academy of Sciences of Belarus.
27, Akademicheskaya Str., 220072, Minsk
E. V. Chepeleva
Belarusian State University
Belarus
Belarus
Elizaveta V. Chepeleva – Student, Belarusian State University.
4, Nezavisimosti Ave., 220030, Minsk
N. V. Kozel
LLC “Biotechnological scientific center”, Industrial Park “Great Stone”; Peasant (farm) economy “Serebryanyj ruchej”
Belarus
Belarus
Nikolai V. Kozel – Ph. D. (Biol.), Аssociate Рrofessor, Leading Research, LLC “Biotechnological scientific center”, Industrial Park “Great Stone”; Chief technologist, Peasant (farm) economy “Serebryanyj ruchej”.
18, Pekinskii Ave., 222210, Smaliavichy District, Minsk Region; Sasnovaja Village, 222202, Aziarycka-Slabadski village council, Smaliavichy District, Minsk Region
References
1. Raja R., Hemaiswarya S., Rengasamy R. Exploitation of Dunaliella for β-carotene production. Applied Microbiology and Biotechnology, 2007, vol. 74, no. 3, pp. 517–523. https://doi.org/10.1007/s00253-006-0777-8
2. Solovchenko A. E., Selivanova E. A., Chekanov K. A., Sidorov R. A., Nemtseva N. V., Lobakova E. S. Induction of secondary carotenogenesis in new halophile microalgae from the genus Dunaliella (Chlorophyceae). Biochemistry (Moscow), 2015, vol. 80, no. 11, pp. 1508–1513 (in Russian). https://doi.org/10.1134/S0006297915110139
3. Minyuk G. S., Drobetskaya I. V., Chubchikova I. N., Terent’eva N. V. Unicellular algae as a renewable resource. Mors’kii ekologіchnii zhurnal [Marine ecological journal], 2008, vol. 7, no. 2, pp. 5–23 (in Russian).
4. Al-Muhteseb S. I., Emeish S. Producing Natural Mixed Carotenoids from Dunaliella salina. Journal of Natural Sciences Research, 2015, vol. 5, no. 10, pp. 53–59.
5. Shariati M., Reza Hadi M. Microalgal Biotechnology and Bioenergy in Dunaliella. Progress in Molecular and Environmental Bioengineering – From Analysis and Modeling to Technology Applications, Isfahan, 1, August 2011. Isfahan, 2011, pp. 483–506. https://doi.org/10.5772/19046
6. Mel’nikov S. S., Manankina E. E., Budakova E. A., Shalygo N. V. Catalogue of the genetic fund of economically useful algae species. Minsk, Belaruskaya navuka Publ., 2011. 101 p. (in Russian).
7. Sirenko L. A., Sakevich A. I., Osipov L. F., Lukina L. F., Kuz’menko M. I., Kozitskaya V. N., Velichko I. M., Myslovich V. O., Gavrilenko M. Ya., Arendarchuk V. V., Kirpenko Yu. A. Methods of physiological and biochemical study of algae in hydrobiological practice. Kiev, Naukova dumka Publ., 1975. 248 p. (in Russian).
8. Azarin K., Usatov A., Makarenko M., Kozel N., Kovalevich A., Dremuk I., Yemelyanova A., Logacheva M., Fedorenko A., Averina N. A point mutation in the photosystem I P700 chlorophyll a apoprotein A1 gene confers variegation in Helianthus annuus L. Plant Molecular Biology, 2020, vol. 103, no. 4–5, pp. 373–389. https://doi.org/10.1007/s11103-020-00997-x
9. Forni E., Ghezzi M., Polesello A. HPLC separation and fluorimetric estimation of chlorophylls and pheophytins in fresh and frozen peas. Chromatography, 1988, vol. 26, no. 1, pp. 120–124. https://doi.org/10.1007/bf02268135
10. Milenković S. M., Zvezdanović J. B., Anđelković T. D., Marković D. Z. The identification of chlorophyll and its derivatives in the pigment mixtures: HPLC-chromatography, visible and mass spectroscopy studies. Advanced technologies, 2012, vol. 1, no. 1, pp. 16–24.
11. Rodriguez-Amaya D. B., Kimura M. HarvestPlus Handbook for Carotenoid Analysis. Washington, International Food Policy Research Institute, 2004. 63 p.
12. Korneev D. Yu. Information capabilities of the fluorescence induction method. Kiev, Al’terpres Publ., 2002, 188 p. (in Russian).
13. Dual-PAM-100 Measuring system for simultaneous assessment of p700 and chlorophyll fluorescence. Heinz Walz GmbH. Germany, 2009. Available at: http://www.walz.com/downloads/manuals/dual-pam-100/Dual-PAM_1e.pdf (accessed 11.04.2020).
14. Govindjee. Sixty-three years since Kautsky chlorophyll a fluorescence. Australian Journal of Plant Physiology, 1995, vol. 22, no. 2, pp. 131–160. https://doi.org/10.1071/pp9950131
15. Chepeleva E. V., Samovich T. V., Kozel N. V. The pigment composition of Dunaliella salina during cultivation on modified media. Biologicheski-aktivnye veshchestva prirodnogo proiskhozhdeniya v regulyatsii protsessov zhiznedeyatel’nosti: sbornik statei Mezhdunarodnoi nauchno-prakticheskoi konferentsii, posvyashchennoi 50-letiyu Instituta biokhimii biologicheski aktivnykh soedinenii Natsional’noi akademii nauk Belarusi (5–6 oktyabrya 2021 goda, g. Grodno, Respublika Belarus’) [Biologically active substances of natural origin in the regulation of life processes: collection of articles from the International scientific and practical conference dedicated to the 50th anniversary of the Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus (October 5–6, 2021, Grodno, Republic of Belarus)]. Grodno, 2021. pp. 569–573 (in Russian).
16. Kalyaga T. G., Kozel N. V. Influence of soil drought on the content of photosynthetic pigments in barley plants of the Brovar variety. Zhurnal Belorusskogo gosudarstvennogo universiteta. Biologiya. [Journal of the Belarusian State University. Biology], 2020, no. 3, pp. 46–53 (in Russian).
17. Liu X., Yin Ch., Xiang L., Jiang W., Xu Sh., Mao Z. Transcription strategies related to photosynthesis and nitrogen metabolism of wheat in response to nitrogen deficiency. BMC Plant Biology, 2020, vol. 20, art. 448. https://doi.org/10.1186/s12870-020-02662-3
Review
Views:
24
Email this article 
