Construction of Escherichia coli strain producing CpG- enriched recombinant plasmid
Abstract
As a result of current work the plasmid pCpG-KH11 carrying 104 copies of CpG motif GTCGTT has been constructed. The cloned motif is considered to be the most effective in stimulation of the human immune system. The constructed plasmid carries the greatest amount of CpG-motifs among the well-known analogs. Using methods of genetic engineering E. coli CpG-KH11 strain has been obtained. The strain is potent in pCpG-KH11 production and makes it possible to produce about 7.7 mg of target plasmid DNA per 1 L of culture broth.
About the Authors
A. S. Shchokolova
Institute of Microbiology, National Academy of Sciences of Belarus
Belarus
Belarus
S. V. Kvach
Institute of Microbiology, National Academy of Sciences of Belarus
Belarus
Belarus
A. I. Zinchenko
Institute of Microbiology, National Academy of Sciences of Belarus
Belarus
Belarus
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