Antifungal potential of some proteins agricultural plants
https://doi.org/10.29235/1029-8940-2020-65-1-50-58
Abstract
The results of studies on the presence in the seeds of legumes and cereals of protein inhibitors that are active against animal proteinases (trypsin) and exogenous peptidases of phytopathogenic microorganisms are presented. It has been shown that secreted proteolytic enzymes of the studied phytopathogens are mainly represented by cysteine proteinases, to a lesser extent, serine and aspartane proteinases are present. It has been established that a close positive correlation between plant resistance to pathogens is observed not with well-known and widespread trypsin inhibitors, but with the activity of inhibitors directed against exogenous peptidases secreted by fungal pathogens of the genus Fusarium, Colletotrichum and Helminthosporium. The results obtained in the course of the work can be used in breeding and genetic studies on the creation of varieties and types of crops with increased resistance to pathogenic microflora and insect pests, as well as to create protective preparations.
Keywords
phytopathogenic microorganisms,
exogenous peptidase inhibitors,
cereals and legumes,
correlation coefficients,
resistance to pathogen
Supporting agencies: This work was supported by the Belarusian and Russian Foundation for Basic Research (grants no. B18R-053 and no. 18-54-00008-Bel.).
About the Authors
V. I. Domash
Institute of Experimental Botany of the National Academy of Sciences of Belarus
Belarus
Belarus
Valentina I. Domash – D. Sc. (Biol.), Chief researcher
27, Akademicheskaya Str., 220072, Minsk
M. A. Belozersky
А. N. Belozersky Institute of Physico-Chemical Biology of the Mosсow State University
Russian Federation
Russian Federation
Mikhail A. Belozersky – Professor, Head of the Department
1, build. 40, Leninskiye gory, 119992, Moscow
Y. E. Dunaevsky
А. N. Belozersky Institute of Physico-Chemical Biology of the Mosсow State University
Russian Federation
Russian Federation
Yakov E. Dunaevsky – Professor, Chief researcher
1, build 40, Leninskiye gory, 119992, Moscow
O. A. Ivanov
Institute of Experimental Botany of the National Academy of Sciences of Belarus
Belarus
Belarus
Oleg A. Ivanov – Ph. D. (Biol.), Head of the Department
27, Akademicheskaya Str., 220072, Minsk
T. P. Sharpio
Institute of Experimental Botany of the National Academy of Sciences of Belarus
Belarus
Belarus
Tamara P. Sharpio – Researcher
27, Akademicheskaya Str., 220072, Minsk
S. A. Zabreiko
Institute of Experimental Botany of the National Academy of Sciences of Belarus
Belarus
Belarus
Svetlana А. Zabreiko – Researcher
27, Akademicheskaya Str., 220072, Minsk
T. G. Shabashova
Institute of Experimental Botany of the National Academy of Sciences of Belarus
Belarus
Belarus
Tatyana G. Shabashova – Ph. D. (Biol.), Head of the Laboratory
27, Akademicheskaya Str., 220072, Minsk
References
1. Chandrasekaran M., Thangavelu B., Chun S. C., Sathiyabama М. Proteases from phytopathogenic fungi and their importance in phytopathogenicity. Journal of General Plant Pathology, 2016, vol. 82, no. 5, pp. 233–239. https://doi.org/10.1007/s10327-016-0672-9
2. Pavlyukova E. B., Belozerskii M. A., Dunaevskii Ya. E. Extracellular proteolytic enzymes of mycelial fungi (review). Biochemistry, 1998, iss. 8, pp. 1039–1089 (in Russian).
3. Semenova T. A., Dunaevsky Y. E., Beljakova G. A., Borisov B. A., Shamraichuk I. L., Belozersky M. A. Extracellular peptidases as possible markers of fungal ecology. Applied Soil Ecology, 2017, vol. 113, pp. 1–10. https://doi.org/10.1016/j.apsoil.2017.01.002
4. Mercado-Flores Y., Hernandez-Rodriguez C., Ruiz-Herrera J., Villa-Tanaca L. Proteinases and exopeptidases from the phytopathogenic fungus Ustilago maydis. Mycologia, 2003, vol. 95, no. 2, pp. 327–339. https://doi.org/10.2307/3762044
5. Neto Y. A., da Rosa Garzon N. G., Pedezzi R., Cabral H. Specificity of peptidases secreted by filamentous fungi. Bioengineered, 2018, vol. 9, no. 1, pp. 30–37. https://doi.org/10.1080/21655979.2017.1373531
6. Ward O. P. Production of recombinant proteins by filamentous fungi. Biotechnology Advances, 2012, vol. 30, no. 5, pp. 1119–1139. https://doi.org/10.1016/j.biotechadv.2011.09.012
7. Mosolov V. V., Grigor’eva L. I., Valueva T. A. The role of proteolytic enzymes and their inhibitors in plant protection (review). Prikladnaya biokhimiya i mikrobiologiya = Applied of Biochemistry and Microbiology, 2001, vol. 37, no. 2, pp. 131–140 (in Russian).
8. Yamaleev A. M., Mukhsinov V. Kh., Isaev R. F., Yamaleeva A. A., Krivchenko V. I. The activity of protease inhibitors and the resistance of wheat to the pathogen of hard smut. Sel’skokhozyaistvennaya biologiya [Agricultural biology], 1980, vol. 15, no. 1, pp. 143–144 (in Russian).
9. Volchevskaya A. G., Adamovskaya V. G., Levitskii A. P., Vovchuk S. V. The relationship between the level of proteinase inhibitors and the resistance of winter wheat to Fusarioz. Fiziologiya i bioikhimiya kul’turnykh rastenii [Physiology and biochemistry of cultivated plants], 1991, vol. 23, no. 4, pp. 365–370 (in Russian).
10. Mosolov V. V., Grigor’eva L. I., Valueva T. A. Plant proteinase inhibitors as multifunctional proteins (review). Prikladnaya biokhimiya i mikrobiologiya = Applied of Biochemistry and Microbiology, 2001, vol. 37, no. 6, pp. 643–650 (in Russian).
11. Anson M. Z. The estimation of pepsin, trypsin, papain and catepsin with hemoglobin. Journal of General Physiology, 1938, vol. 22, no. 1, pp. 79–89. https://doi.org/10.1085/jgp.22.1.79
12. Erlanger F., Kokowsky N., Cohen W. The preparation and properties of two new chromogenic substrates of trypsin. Archives of Biochemistry and Biophysics, 1961, vol. 95, no. 2, pp. 271–278. https://doi.org/10.1016/0003-9861(61)90145-x
13. Gofman Yu. Ya., Vaisblai I. M. Determination of the activity of trypsin inhibitors in pea seeds. Prikladnaya biokhimiya i mikrobiologiya = Applied of Biochemistry and Microbiology, 1975, vol. 11, no. 5, pp. 777–787 (in Russian).
14. Bradford M. M. A rapid and sensitive method for quantitation of microgramm quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry, 1976, vol. 72, no. 1–2, pp. 248–254. https://doi.org/10.1016/0003-2697(76)90527-3
15. Ibragimov R. I. Suppression of the activity of extracellular proteinases of the pathogenic fungus Fusarium sp. inhibitors from seeds and vegetative organs of plants. Doklady Rossiiskoi akademii sel’skokhozyaistvennykh nauk [Reports of the Russian Academy of Agricultural Sciences], 1997, no. 2, pp. 15–17 (in Russian).
16. Yarullina L. G., Akhatova A. R., Kasimova R. I. Hydrolytic enzymes and their protein inhibitors in the regulation of plant relationships with pathogens. Fiziologiya rastenii [Plant physiology], 2016, vol. 63, no. 2, pp. 205–217 (in Russian).
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