Registration of entomopatogenic micromycete Cordyceps sp. in bloodsucking blackflies Wilhelmia equine (Linnaeus, 1758)
https://doi.org/10.29235/1029-8940-2020-65-1-43-49
Abstract
An entomopathogenic fungi belonging to the genus Cordyceps was isolated from the affected larvae of the bloodsucking blackflies Wilhelmia equina (Linnaeus, 1758). Larval stage of Simulium were collected on the territory of the Lepel district of the Vitebsk region in the river Essa in July 2018. Isolate was identified using cultural-morphological and molecular-genetic methods.
In vitro experiments comparative evaluation of biological activity of isolated strain Cordyceps sp. and Beauveria bassiana 10-06 was carried out for 3rd instar larvae of W. equina. Two different concentrations 1∙106 and 1∙107 conidia/ml were made and tested in 3 replications. On the 3rd day of incubation both fungi caused a significant mortality of test-objects (p = 0.03) compared to control. The biological efficiency of the concentration of 107 соnidia/ml of the strain Beauveria bassiana 10-06 on the 3rd day was 94.4 %, on the 4th – 98.3 %, of the strain Cordyceps sp. – 76.0 and 88.9 % respectively.
Keywords
entomopathogenic fungi,
Ascomycetes,
Simuliidae,
Cordycipitaceae,
Isaria,
Beauveria bassiana,
biological efficiency
Supporting agencies: The work was carried out with the financial support of grant of NASB № 2019-28-103 of April 15, 2019.
About the Authors
D. V. Dovnar
Scientific and Practical Center for Bioresources of the National Academy of Sciences of Belarus
Belarus
Belarus
Darya V. Dovnar – Junior researcher
27, Akademicheskaya Str., 220072, Minsk
E. N. Yankovskaya
Institute of Plant Protection of the National Academy of Sciences of Belarus
Belarus
Belarus
Elena N. Yankovskaya – Ph. D. (Biol.), Leading researcher
2, Mir Str., 223011, a/c Priluki, Minsk District
D. V. Voitka
Institute of Plant Protection of the National Academy of Sciences of Belarus
Belarus
Belarus
Dmitry V. Voitka – Ph. D. (Biol.), Head of the Laboratory
2, Mir Str., 223011, a/c Priluki, Minsk District
O. Yu. Baranov
Forest Institute of the National Academy of Sciences of Belarus
Belarus
Belarus
Oleg Y. Baranov – D. Sc. (Biol.), Assistant professor, Head of the Department
71, Proletarskaya Str., 246050, Gomel
V. M. Kaplich
Belarusian State Technological University
Belarus
Belarus
Valeriy M. Kaplich – D. Sc. (Biol.), Professor
13a, Sverdlov Str., 220006, Minsk
H. V. Kanterova
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Belarus
Hanna V. Kanterova – Researcher
2, Kuprevich Str., 220141, Minsk
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