Chromatographic purification of the enzyme preparation from the cultural liquid of Pleurotus ostreatus
https://doi.org/10.29235/1029-8940-2019-64-4-467-471
Abstract
The primary purification of the proteolytic enzyme preparation from the Pleurotus ostreatus culture fluid was carried out. Methods of salting out, dialysis, ion-exchange chromatography on DEAEand KM-sepharose were used. It was shown that during the chromatography on KM-sepharose, the enzyme preparation from the culture liquid was divided into three fractions, one of which possessed milk-clotting activity (МСА). Chromatography on DEAE-sepharose allowed us to reach the significant purity of the enzyme with МСА.
About the Authors
V. V. Sakovich
Polessky State University
Belarus
Belarus
Valeryia V. Sakovich – Master of biological sciences
23, Dneprovskaya Flotiliya Str., 225710, Pinsk
A. M. Hrusha
Polessky State University
Belarus
Belarus
Aliaksandr M. Hrusha – Student
23, Dneprovskaya Flotiliya Str., 225710, Pinsk
V. V. Revenko
Polessky State University
Belarus
Belarus
Vadim V. Revenko – Student
23, Dneprovskaya Flotiliya Str., 225710, Pinsk
D. D. Zhernossekov
Polessky State University
Belarus
Belarus
Dmitri D. Zhernossekov – D. Sc. (Biol.), Assistant Professor
23, Dneprovskaya Flotiliya Str., 225710, Pinsk
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