Efficient strain of slow-growing nodulating bacteria Bradyrhizhobium japonicum 84KL as a basis of biofertilizer Soyarhiz

Abstract

Most efficient competitive strain of slow-growing nodulating bacteria Bradyrhizhobium japonicum 84KL possessing growth-promoting and nitrogen-fixing activities was isolated and selected from nodules of Glycine max. cultivated in Belarus. Technological parameters and conditions for submerged fermentation of a new bacterial strain were defined, and survival rate of the strain during storage in liquid culture and upon immobilization on substrate-carrier was examined. It was found that pre-sowing inoculation of soya seeds with biofertilizer Soyarhiz promoted rise in soya seed productivity stimulated by mineral P50 K120 supply by 7.8 c/ha, 5.4 % increase in seed protein content, accumulation of ammonium nitrogen in soil in amount 4.3-6.3 mg/kg with concomitant fall of nitrate nitrogen level by 60-70 %.