Design of genetic construction for creation DNA vaccine against porcine reproductive and respiratory syndrome
https://doi.org/10.29235/1029-8940-2018-63-4-419-425
Abstract
The porcine reproductive and respiratory syndrome (PRRS) caused the serious economic damage to swine breeding around the world. It is a viral infective disease against which live attenuated and inactivated vaccines are not always successful. Development of new types of drugs such as DNA vaccines is necessary for improving the protection against the virus. DNA vaccines induce the development of both a cellular and humoral immune response. Such vaccines consist of a plasmid or viral vector with genes of potentially immunogenic proteins. The expression of these genes realized in cells of the vaccinated animal. It leads to the synthesis of antigen proteins triggering the immune response. The purpose of this work is to create a genetic construction that can be used as DNA vaccine against PRRS virus. The construction consists of the commercial vector pVAX1 and open reading frame of two structural proteins of PRRS virus, a lysosomal localization signal sequence of the invariant chain gene and regulatory elements necessary for the expression of cloned genes in mammalian cells.
About the Authors
L. M. Kravchenko
Belarusian State University
Belarus
Belarus
Lidia M. Kravchenko – Junior researcher.
4, Nezavisimosti Ave., 220030, Minsk.
K. V. Kudzin
Belarusian State University
Belarus
Belarus
Kiryl V. Kudzin – Researcher.
4, Nezavisimosti Ave., 220030, Minsk.
U. A. Prakulevich
Belarusian State University
Belarus
Belarus
Uladzimir A. Prakulevich – D. Sc. (Biol.), Professor, Head of the Department.
4, Nezavisimosti Ave., 220030, Minsk.
References
1. Liu M. A. DNA vaccines: a review. Journal of Internal Medicine, 2003, vol. 253, no. 4, pp. 402–410.
2. Shedlock D. J., Weiner D. B. DNA vaccination: antigen presentation and the induction of immunity. Journal of Leukocyte Biology, 2000, vol. 68, no. 6, pp. 793–806.
3. Coban C., Koyama S., Takeshita F., Akira S., Ishii K. J. Molecular and cellular mechanisms of DNA vaccines. Human Vaccines, 2008, vol. 4, no. 6, pp. 453–457. https://doi.org/10.4161/hv.4.6.6200
4. Labarque G., van Reeth K., Nauwynck H., Drexler C., van Gucht S., Pensaert M. Impact of genetic diversity of European-type porcine reproductive and respiratory syndrome virus strains on vaccine efficacy. Vaccine, 2004, vol. 22, no. 31–32, pp. 4183–4190. https://doi.org/10.1016/j.vaccine.2004.05.008
5. Scortti M., Prieto C., Alvarez E., Simarro I., Castro J. M. Failure of an inactivated vaccine against porcine reproductive and respiratory syndrome to protect gilts against a heterologous challenge with PRRSV. Veterinary Record, 2007, vol. 161, no. 24, pp. 809–813.
6. Petrini S., Ramadori G., Villa R., Borghetti P., de Angelis E., Cantoni A. M., Corradi A., Amici A., Ferrari M. Evaluation of different DNA vaccines against porcine reproductive and respiratory syndrome (PRRS) in pigs. Vaccines, 2013, vol. 1, no. 4, pp. 463–480. https://doi.org/10.3390/vaccines1040463
7. Murthy A., Ni Y., Meng X., Zhang Ch. Production and evaluation of virus-like particles displaying immunogenic epitopes of porcine reproductive and respiratory syndrome virus (PRRSV). International Journal of Molecular Sciences, 2015, vol. 16, no. 12, pp. 8382–8396. https://doi.org/10.3390/ijms16048382
8. Lu Z. H., Archibald A. L., Ait-Ali T. Beyond the whole genome consensus: unravelling of PRRSV phylogenomics using next generation sequencing technologies. Virus Research, 2014, vol. 194, pp. 167–174. https://doi.org/10.1016/j.virusres.2014.10.004
9. Rossow K. D. Porcine reproductive and respiratory syndrome. Veterinary Pathology, 1998, vol. 35, no. 1, pp. 1–20.
10. Wang R. Induction of antigen-specific cytotoxic t lymphocytes in humans by a malaria DNA vaccine. Science, 1998, vol. 282, no. 5388, pp. 476–480. https://doi.org/10.1126/science.282.5388.476
11. Théry C., Brachet V., Regnault A., Rescigno M., Ricciardi-Castagnoli P., Bonnerot C., Amigorena S. MHC class II transport from lysosomal compartments to the cell surface is determined by stable peptide binding, but not by the cytosolic domains of the β- and α-chains. Journal of Immunology, 1998, vol. 161, no. 5, pp. 2106–2113.
12. Starodubova E. S., Isaguliants М. G., Kuzmenko Y. V., Latanova A. A., Krotova О. А., Karpov V. L. Fusion to the lysosome targeting signal of the invariant chain alters the processing and enhances the immunogenicity of HIV-1 reverse transcriptase. Acta Naturae, 2014, vol. 6, no. 1, pp. 61–68.
13. Ausubel F. M., Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. (eds.). Current protocols in molecular biology. 2nd ed. New York, John Wiley & Sons, 1993. 4410 p.
14. Nam H.-M., Chae K.-S., Song Y.-J., Lee N.-H., Lee J.-B., Park S.-Y., Song Ch.-S., Seo K.-H., Kang S.-M., Kim M.-Ch., Choi I.-S. Immune responses in mice vaccinated with virus-like particle composed of the GP5 and M proteins of porcine reproductive and respiratory syndrome virus. Archives of Virology, 2013, vol. 158, no. 6, pp. 1275–1285. https://doi.org/10.1007/s00705-013-1612-z
15. Wootton S. K., Nelson E. A., Yoo D. Antigenic structure of the nucleocapsid protein of porcine reproductive and respiratory syndrome virus. Clinical and Diagnostic Laboratory Immunology, 1998, vol. 5, no. 6, pp. 773–779.
16. Zhang X., Wang X., Mu L., Ding Z. Immune responses in pigs induced by recombinant DNA vaccine co-expressing swine IL-18 and membrane protein of porcine reproductive and respiratory syndrome virus. International Journal of Molecular Sciences, 2012, vol. 13, no. 5, pp. 5715–5728. https://doi.org/10.3390/ijms13055715
17. Chia M.-Y., Hsiao S.-H., Chan H.-T., Do Y.-Y., Huang P.-L., Chang H.-W., Tsai Y.-C., Lin C.-M., Cheng C.-H., Pang V. F., Jeng C.-R. Improved immunogenicity of DNA constructs co-expressing the GP5 and M proteins of porcine reproductive and respiratory syndrome virus by glycine-proline-glycine-proline (GPGP) linker in mice. Taiwan Veterinary Journal, 2011, vol. 37, no. 1, pp. 12–23.
18. Zheng Q., Chen D., Li P., Bi Z., Cao R., Zhou B., Chen P. Co-expressing GP5 and M proteins under different promoters in recombinant modified vaccinia virus Ankara (rMVA)-based vaccine vector enhanced the humoral and cellular immune responses of porcine reproductive and respiratory syndrome virus (PRRSV). Virus Genes, 2007, vol. 35, no. 3, pp. 585–595. https://doi.org/10.1007/s11262-007-0161-5
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