ANALYSIS OF INDIVIDUAL CLADOCERA AND COPEPODS FROM MESOTROPHIC LAKE USING THE PHENOM PROX-SEM/EDS
Abstract
The stoichiometric analysis of Cladocera and Copepods from four different habitats of Obsterno mesotrophic lake in Belarus have been conducted in September 2016 using a method based on X-ray microanalysis, Phenom Prox Scanning Electron Microscope (SEM) with an elemental detection system (EDS), for measurement of atomic weight fractions of carbon, nitrogen and phosphorus elements in a designated area of zooplankton tissues. Phenom Prox SEM/EDS provided atomic weight percent of C:N, C:P, N:P ratios for Cladocera in pelagial, rush beds and nymphaea and for Copepods in pelagial, bare littoral, rush beds and nymphaea respectively. For Cladocera, the content of carbon in tissues was significantly higher in pelagial than in rush beds and nymphaea habitats (p < 0.01), opposite to nitrogen and phosphorous which contents were significantly lower in the pelagial (p < 0.01) comparing with other habitats. In the case of Copepods contents of carbon and nitrogen in their tissues did not differ between habitats. Significant among habitats differences were found for phosphorus – its content was significantly higher in Copepods from pelagial than from nymphaea habitat (p < 0.01).The purpose of this research is to determine the main biochemical elements content in zooplankton samples for different biotopes and to assess the elemental composition.
About the Authors
Shabnam G. Farahani
Scientific and Practical Center for Bioresources of the National Academy of Sciences of Belarus
Belarus
Postgraduate student
Belarus
Postgraduate student
A. Wojtal-Frankiewicz
University of Lodz
Poland
Professor, Director
Poland
Professor, Director
P. Frankiewicz
University of Lodz
Poland
Professor
Poland
Professor
Zh. Buseva
Scientific and Practical Center for Bioresources of the National Academy of Sciences of Belarus
Belarus
Ph. D. (Biol.), Senior researcher
Belarus
Ph. D. (Biol.), Senior researcher
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