ANALYSIS OF MICROSATELLITE LOCI VARIABILITY RIBES L. REPRESENTATIVES GROWN IN BELARUS

The study of the genetic diversity of 86 representatives of the genus Ribes cultivated in Belarus using 8 SSR markers was held. It is shown that the varieties of black currant, red currant and gooseberry grown in Belarus, is characterized by high diversity of microsatellite loci. The average number of alleles per locus among the 86 samples was 12.1. The average number of unique genotypes per marker was 26.4. The value of discriminatory power for all markers was high with average number 0.8. Samples belonging to different species formed distinct clusters in the dendrogram of phylogenetic similarity. Genetically closest to black currant were josta, Golden currant, Alpine currant. At a greater genetic distance were red currants. Varieties of gooseberries form a cluster that is most distant from currant species. Analysis of alleles distribution at the investigated loci of microsatellite sequences revealed that red currant and gooseberry varieties have different SSR-alleles from black currant varieties. The number of alleles detected only for red currant and gooseberry varieties ranged from 1 (loci g2-G12, g1-M0) to 5 (locus E4D03). Based on the analysis of SSR-locus polymorphism, a set of 8 markers was generated that allow DNA-identification of Ribes genotypes. For selection the set of markers the level of information value for each marker, the frequency of alleles occurrence and convenience of visualization and analysis of amplification products were taken into account. The method of SSR-analysis with using specified set of markers can be successfully applied for identification of black currant, red currant and gooseberry varieties at the molecular level.

black currant, redcurrant, gooseberry SSR-markers, genetic variability, DNA-identification

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