Analysis of variability of quantitative traits at regenerants of Vaccinium corymbosum in vitro under the action of phytosteroids
Abstract
Results of the comparative analysis of variability of 8 quantitative traits at regenerants of Northland and Patriot cultivars of highbush blueberry in vitro on nutrient, agarized mediums for rooting, with organic compounds, on macro- and micro- salt basis of V WPM differing on composition of phytohormonal steroids in a combination with IAA in 15 variants of experience are given in the present article. Usage of phytohormones in comparison with control led to authentic increase in indicators of regenerants height in 1.2-1.8 times; lengths of the third interstice in 1.2-1.4 times; the quantities of shoots, weight of regenerants and quantities of leaves in 1.2-1.3 times; explants viability in 1.2-1.5 times. In overwhelming majority of cases the presence of phytohormonal steroids - EB or EK - leads to authentic, essential rooting reduction in 1.2-5.7 times at both investigated cultivars. It is established the authentic (in most cases at P < 0.01) influence of a genotype on variability of all analyzed traits, as well as the authentic at P < 0.01 influence of phytohormonal structure of medium on variability of practically all traits (except for weight of regenerants).
About the Authors
O. A. Kudryashova
Полесский государственный университет
Belarus
Belarus
A. A. Volotovich
Полесский государственный университет
Belarus
Belarus
E. P. Gleb
Полесский государственный университет
Belarus
Belarus
E. V. Sakhvon
Полесский государственный университет
Belarus
Belarus
P. S. Minin
Институт биоорганической химии НАН Беларуси
Belarus
Belarus
R. P. Litvinovskaia
Институт биоорганической химии НАН Беларуси
Belarus
Belarus
V. A. Khripach
Институт биоорганической химии НАН Беларуси
Belarus
Belarus
References
1. Hayat S., Ahmad A. Brassinosteroids: A Class of Plant Hormone. Berlin: Springer-Verlag, 2010.
2. Braun P., Wild A. // J. Plant Physiol. 1984. Vol. 116. P. 189-196.
3. Wang Y., Luo W., Zhao Y., Ikekawa N. // Zhiwu Shenglixue Tongxun. 1988. Vol. 1. P. 29-31.
4. Schilling G., Schiller C., Otto S. // Brassinosteroids. Chemistry, Bioactivity and Applications. ACS Symposium Series. 1991. Vol. 474. P. 208-219.
5. Mironenko A. V., Kandelinskaya O. L., Bushueva S. A., Uralskaya H. R. // Dokl. RAAS. 1996. Vol. 1. P. 11-13.
6. Mironenko A. V., Kandelinskaya O. L., Chekhova A. N. et al. Problems of experimental botany. Minsk, 1997.
7. Kandelinskaya O. L., Khripach V. A. // Int. Workshop. Brassinosteroids: Chemistry, Bioactivity, Application. Halle, 1990. P. 46.
8. Deeva V. P., Sanko N. V., Vedeneev A. N., Pavlova I. V. Problems of experimental botany. Minsk, 1997.
9. Katsumi M. // Brassinosteroids. Chemistry, Bioactivity and Applications. ACS Symposium Series. 1991. Vol. 474. P. 246-254.
10. Wilen R. W., Sacco M., Gusta L. V., Krishna P. // Physiol. Plant. 1995. Vol. 95. P. 195-202.
11. Hirai K., Fujii S., Honjo K. // Jpn. J. Crop Sci. 1991. Vol. 60. P. 29-35.
12. Kulaeva O. N., Burkhanova E. A., Fedina A. B. et al. // Brassinosteroids. Chemistry, Bioactivity and Applications. ACS Symposium Series. 1991. Vol. 474. P. 141-155.
13. Bokebayeva G. A., Khripach V. A. // Brassinosteroids - biorational, ecologically safe regulators of growth and productivity of plants. Minsk, 1993. P. 21.
14. Zhao Y.-J., Wu D. // Int. Workshop. Brassinosteroids: Chemistry, Bioactivity, Application. Halle, 1990. P. 26.
15. Mussig C., Altmann T. // Plant Physiol. Biochem. 1999. Vol. 37. P. 363-372.
16. Hardtke Ch. S., Dorcey E., Osmont K. S., Sibout R. // Trends Cell Biol. 2007. Vol. 17. P. 485-492.
17. Yin Y., Wang Zh., Mora-Garcia S., Li J. et al. // Cell. 2002. Vol. 109. P. 181-191.
18. Yin Y., Vafeados D., Tao Y. et al. // Cell. 2005. Vol. 120. P. 249-259.
19. Yopp J. H., Mandava N. B., Sasse J. M. // Physiol. Plant. 1981. Vol. 53. P. 445-452.
20. Takeno K., Pharis R. P. // Plant Cell Physiol. 1982. Vol. 23. P. 1275-1281.
21. Arteca R. N., Tsai D. S., Schlagnhaufer C., Mandava N. B. // Physiol. Plant. 1983. Vol. 59. P. 539-544.
22. Meudt W. J., Thompson M. J. // Proc. Plant Growth Regul. Soc. Am. 1983. Vol. 10. P. 306-311.
23. Choi C.-D., Kim S.-C., Lee S.-K. // Korean J. Crop Sci. 1990. Vol. 35. P. 58-64.
24. Cao H., Chen S. // Plant Growth Regul. 1995. Vol. 16. P. 189-196.
25. Meudt W. J. // Plant. Physiol. 1987. Vol. 83. P. 195-198.
26. Arteca R. N. // Physiol. Plant. 1984. Vol. 62. P. 102-104.
27. Arteca R. N., Bachman J. M., Yopp J. H, Mandava N. B. // Physiol. Plant. 1985. Vol. 64. P. 13-16.
28. Schlagnhaufer C. D., Arteca R. N. // Plant. Physiol. 1985. Vol. 77. P. 157.
29. Arteca R., Schlagnhaufer C. // Physiol. Plant. 1984. Vol. 62. P. 445-447.
30. Schlagnhaufer C., Arteca R. N., Yopp J. H. // Physiol. Plant. 1984. Vol.61. Vol. 555-558.
31. Arteca R. N., Tsai D. S., Mandava N. B. // J. Plant Physiol. 1991. Vol. 139. P. 52-56.
32. Kozick T. A., Kislin E. N. // Workshop on Brassinosteroids. 1991. P. 29-30.
33. Kurapov P. B. Hormonal balance in plants. Doctor of Sciences Degree Thesis. Moscow: Tymyryazev Agricultural Academy, 1996.
34. Luo B., Yu D., Zhou D. // Plant Physiol. Commun. 1988. P. 31-34.
35. Khripach V. A., Zhabinskii V. N., Groot A. E. Brassinosteroids. A new class of plant hormones. San Diego: Academic Press, 1999.
36. Gregory L. E, Mandava N. B. // Physiol. Plant. 1982. Vol. 54. P. 239-243.
37. Zhou A.-Q. // Plant Physiol. Communs. 1987. P. 19-22.
38. Кудряшова О. А., Волотович А. А., Хрипач В. А. и др. Физиол. растен. 2012. Т. 59, № 4. С. 632-640.
39. Romani G., Marre M. T, Bonetti A. etal. // Physiol. Plant. 1983. Vol. 59. P. 528-532.
40. Cerana R., Spelta M., Bonetti A., Lado P. // Plant Sci. 1985. Vol. 38. P. 99-105.
41. Sathiyamoorthy P., Nakamura S. // Plant Growth Regul. 1990. Vol. 9. P. 73-76.
42. Guan M., Roddick J. G. // Physiol. Plant. 1988. Vol. 73. P. 426-431.
43. Roddick J. G., Rijnenberg A. L., Ikekawa N. // Physiol. Plant. 1993. Vol.87. P. 453-458.
44. Roddick J. G. // Phytochemistry. 1994. Vol. 37. P. 1277-1281.
45. Wang S.-G., Deng R.-F. // J. Southwest Agric. Univ. 1992. Vol. 14. P. 177-181.
46. Рупасова Ж. А., Яковлев А. П. Фиторекультивация выбывших из промышленной эксплуатации торфяных месторождений севера Беларуси на основе возделывания ягодных растений семейства Ericaceae / Под ред. В. Н. Решетникова. Мн., 2011.
47. Сидорович Е. А., Кутас Е. Н. Клональное микроразмножение новых плодово-ягодных растений. Мн., 1996.
48. Trigiano R. N., Gray D.J. Plant tissue culture concepts and laboratory exersises. US/MA, CRC Press LLC. 1999-2000.
49. Доспехов Б. А. Методика полевого опыта. М., 1985.
50. Боровиков В. П. STATISTICA: Искусство анализа данных на компьютере. СПб., 2001.
51. Аношенко Б. Ю. // Генетика. 1994. Т. 30. С. 8-9.
Review
Views: 474
Email this article 
