PROINFLAMMATORY CYTOKINES SYNTHESIS AND ADHESION MOLECULES EXPRESSION BY NEUTROPHILS IN VITRO IN RESPONSE TO ACTIVATOR BASED ON SACCHAROMYCES CEREVISIAE

Immunomodulation is a modulation (regulatory adjustment) of the immune system at the return of the immune status to the normal level. Of particular interest are the immunomodulatory drugs of microbial origin. Under the influence of these drugs are enhanced functional properties of phagocytes (increased phagocytosis and intracellular killing of bacteria absorbed), increased production of proinflammatory cytokines, required for the initiation of humoral and cellular immunity. The article presents the results of studing activity of the biological activator obtained from Saccharomyces cerevisiae cell. Activator induse the synthesis of proinflammatory cytokines (TNF-α, IL-1β, IL-6, IL-8) by blood donor’s cells. As a result, that isolated from yeast cell lysate glycoprotein has the ability to induce synthesis of proinflammatory cytokines blood cells, largely induced synthesis of IL-8. These results indicate that isolated from yeast cell lysate glycoprotein has the ability to activate neutrophils donors by changing expression adhesion markers (CD162, CD177) and cell activation (CD69, CD281, CD282, CD286). Under the influence of the glycoprotein Saccharomyces cerevisiae on neutrophils occurs as a significant increase in the percentage of CD281+282+ cells and CD282+286+ cells. After the interaction of blood cells with the yeast glycoprotein, the percentage of neutrophils expressing CD162 reduced significantly, thus there is a significant increase in cells expressing the CD177 marker.

immunomodulatory, cytokine, glycoprotein, neutrophils, differentiation markers

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