METHODOLOGICAL APPROACH TO THE STUDY OF THE DYNAMIC OF ANTIGENS SYNTHESIS AND SECRETION BY NONPATHOGENIC ORGANISMS ON THE EXAMPLE OF LACTOCOCCUS LACTIS
Abstract
Dynamics of synthesis and distribution in the system cell-environment of lactococcal antigens (4 strains) was investigated by direct and competitive ELISA. It was found that lactococcal antigens include intracellular, cell wall and secretory biopolymers. It was shown that lactococcal antigens are synthesized and distributed in the system cell-environment in dependence on strain, stage of culture growth and media composition. The data will find an application in biotechnology of probiotics.
About the Authors
E. Kiseleva
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Ph. D. (Chem.), Liding Researcher
A. Ezybets
Byelarusian State University
Belarus
Student
Belarus
Student
K. Mikhailopulo
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Researcher
Belarus
Researcher
G. Novik
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Belarus
Ph. D. (Biol.), Head of the Laboratory “Collection of Microorganisms”
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