USING WASTE STRAW SUBSTRATE AFTER OYSTER MUSHROOM CULTIVATION FOR FEEDING OF YOUNG CATTLE
Abstract
Content of protein, cellulose and lignin in the straw substrate at different stages of oyster mushroom large-scale cultivation have been investigated. After whole cycle of Pleurotus ostreatus growth in the solid-state culture quantity of crude protein and true protein in the substrate increased 3.3 and 2.8 times respectively. The content of insoluble fibers in the straw decreased 3 times, quantity of lignin reduced 1.4 times. Activity of enzymes in the substrate after 2–3 waves of fructification was the following (U/g): cellulase – 23.4–27.3, manganese peroxidase – 0.11–0.23, laccase – 0.87–1.39. It was established that feeding of calves straw waste of oyster mushroom industrial cultivation cause positive effect on consumption of diet nutrients. Presence in the waste substrate of cellulolytic and lignolytic enzymes opens new opportunities in fodder manufacturing to promote digestibility of forages.
About the Authors
T. A. Puchkova
Institute of Microbiology of the National Academy of Sciences
Belarus
Belarus
Ph. D. (Biol.), associate professor, faculty of biology
A. A. Kastsianevich
Institute of Microbiology of the National Academy of Sciences
Belarus
Belarus
Ph. D. (Biol.), head of laboratory of protein
I. A. Goncharova
Institute of Microbiology of the National Academy of Sciences
Belarus
Belarus
Ph. D. (Biol.), Leading Researcher
A. I. Kozinets
The Scientific and Practical Center for Animal Breeding of the National Academy of Sciences
Belarus
Belarus
P h. D . (Agr.), head of laboratory of feed additives and bioproducts
References
1. Patil, S. S., Ahmed, S. A., Telang, S. M. and Baig, M. M. V. (2010) ‟The nutritional value of Pleurotus ostreatus (Jacq.: Fr.) Kumm cultivated on different lignocellulosic agrowastes”, Innovative Romanian Food Biotechnology, vol. 7, pp. 66–76.
2. Akyüz, M. and Kirbağ, S. (2010) ‟Effect of various agro-residues on nutritive value of Pleurotus eryngii (DC. Ex Fr.) Quel. Var. ferulae Lanzi”, Journal of Agricultural Science, vol. 16, pp. 83-88.
3. Jafarpour, M. and Eghbalsaeed, S. (2012) ‟High protein complementation with high fiber substrates for oyster mushroom cultures”, African Journal of Biotechnology, vol. 11, no. 14, pp. 3284-3289.
4. Golubev, O. (2004) ‟The waste of Oyster mushroom industrial cultivation”, Kombikorma [Compounted Feed], no. 4, p. 42.
5. Masutti, D. C., Borgognone, A. and Setti, L. (2012) ‟Production of enzyme from rice husks and wheat straw in solid state fermentation”, Chemical engineering transactions, vol. 27, pp. 133–138, doi: 103303/CET1227023.
6. Peterburgskii, A. V. (1968) Praktikum po agronomicheskoi khimii [Workshop on agronomical chemistry], Kolos, Moskow, RU.
7. Ermakov, A. I. (ed.) (1987) Metody biokhimicheskogo issledovaniya rastenii [Methods of a biochemical research of plants], Agropromizdat, Leningrad, RU.
8. Zadrazil, F. and Brunnet, H. (1980) ‟The influence of Ammonium Nitrate supplementation on degradation and in vitro digestibility of straw colonized by higher fungi”, European Journal of Applied Microbiology and Biotechnology, vol. 9, no. 1, pp. 37-39.
9. GOST R 53046-2008 (2009) Preparaty fermentnye. Metody opredeleniya fermentativnoi aktivnosti tsellyulazy [State Standard R 53046-2008. Enzyme preparation. Methods of definition of enzymatic activity of cellulase], Standartinform, Moscow, RU.
10. Wariishi, H., Valli, K. and Gold, M. H. (1992) ‟Manganese (II) oxidation by manganese peroxidase from the basidiomycete Phanerochaete chrysosporium. Kinetic mechanism and role of chelators”, The Journal of Biological Chemistry, vol. 267, pp. 23688–23695.
11. Ovsyannikov, A. I. (1976) Osnovy opytnogo dela v zhivotnovodstve [Fundamentals of experimental work in animal husbandry], Kolos, Moscow, RU.
12. Keavsompong, S., Sanomwatanawong, J. and Nitisinprasert, S. (2005) ‟Cellulolytic enzyme system of Pleurotus ostreatus and its application in animal feed”, Mushroom biology and mushroom products (ICMBMP): the Fifth Int. conf., 8–12 April 2005, Shanghai, China, vol. 12, pp. 68-73.
Review
Views: 757
Email this article 
