Efficiency of multiplex RT-PCR for differential detection of blueberry (Vaccinium corymbosum L.) viruses
Abstract
It was found that multiplex RT-PCR (mRT-PCR) method with two combinations of primers (1F/1R, MF05-22-F/MF05-21-R, AtropaNad2.1a/2.2b and RDP-1/RDP-2, MF05-22-F/MF05-21-R, AtropaNad2.1a/2.2b) can be used for the simultaneous and differential detection of blueberry pathogens (Blueberry scorch carlavirus, Tobacco ringspot nepovirus). Application of mRT-PCR for detection of virus diseases of blueberry plants allows to reduce testing costs on 49.5 % in comparison with simplex RT-PCR.
About the Authors
T. N. Bazhydai
Институт плодоводства
Belarus
Belarus
N. N. Valasevich
Институт плодоводства
Belarus
Belarus
N. V. Kukharchik
Институт плодоводства
Belarus
Belarus
References
1. Certification schemes. Pathogen-tested material of Vaccinium spp. EPPO Standards PM 4/18 (1) // Bull. OEPP/EPPO. 1997. Vol. 27. P. 195-204.
2. Menzel W., Zahn V., Maiss E. // J. of Virol. Meth. 2003. Vol. 110. P. 153-157.
3. Hassan M., Myrta A., Polak J. // J. of Virol. Meth. 2006. Vol. 133. P. 124-129.
4. Yu Y., Zhao Z., Jiang D. et al. // Lett. in Appl. Microbiol. 2013. Vol. 57. P. 350-355.
5. Menzel W., Jelkmann W., Maiss E. // J. of Virol. Meth. 2002. Vol. 99. P. 81-92.
6. Thompson J. R, Wetzel S, Klerks M. M. et al. // J. of Virol. Meth. 2003. Vol. 111. P. 85-93.
7. Ji Z., Zhao X., Duan H. et al. // Acta Virol. 2013. Vol. 57. P. 435-441.
8. Yao B., Wang G., Ma X. et al. // J. of Virol. Meth. 2014. Vol. 196. P. 113-119.
9. Noorani M. S., Awasthi P., Sharma M. P. et al. // J. of Virol. Meth. 2013. Vol. 193. P. 103-107.
10. Jarosova J., Kundu J. K. // Scientia Horticulturae. 2010. Vol. 125. P. 68-72.
11. Liu F., Feng L., Chen X. et al. // J. of Phytopathol. 2012. Vol. 160. P. 622-627.
12. Bertolini E., Olmos A., Martinez M. C. et al. // J. of Virol. Meth. 2001. Vol. 96. P. 33-41.
13. Ali R. N., Dann A. L., Cross P. A., Wilson C. R. // Arch. of Virol. 2014. Vol. 159. P. 3095-3099.
14. Tuo D., Shen W., Yang Y. et al. // Viruses. 2014. Vol. 6. P. 3893-3906.
15. Wegener L. A. // Epidemiology and strain identification of blueberry scorch virus on highbush blueberry in British Columbia: thesis.. master of science in plant virology. Burnaby, 2006.
16. Paduch-Cichal E., Chodorska M., Kalinowska E., Komorowska B. // Acta Sci. Pol., Hortorum Cultus. 2014. Vol. 13, N 3. P. 3-11.
17. Fuchs M., Abawi G. S., Marsella-Herrick P. et al. // J. of Plant Pathol. 2010. Vol. 92, N 2. P. 451-459.
Review
Views: 564
Email this article 
