Identification of antigenic activity apple mosaic virus method of DAS-ELISA-test after storage of hop samples (Humulus lupulus)
Abstract
The results of ApMV diagnostics efficiency with using frozen leaves as the tested material were presented in the article. It was determined that after leaves storage at -20 °C optical density did not differ significantly from using of fresh material. Conservation of antigenic activity didn’t depend on host plant cultivar. The estimation of ELISA-test susceptibility using the range of cell sap dilution showed that using the plant sample with high concentration of virus made possible to detect ApMV in highly diluted solution (up to 1:1000). Storage of plant extracts from frozen leaves after homogenization influenced negatively on viral capacity to react with cover antibodies.
About the Authors
O. A. Hashenka
Институт плодоводства
Belarus
Belarus
N. N. Volosevich
Институт плодоводства
Belarus
Belarus
E. V. Kolbanova
Институт плодоводства
Belarus
Belarus
M. S. Kastritskaya
Институт плодоводства
Belarus
Belarus
N. V. Kucharchik
Институт плодоводства
Belarus
Belarus
References
1. Pethybridge S. J., Hay F. S., Barbara D. J., Eastwell K. C. et al. // Plant Dis. 2008. Vol. 92, N 3. P. 324-338.
2. Кухарчик Н. В. // Вирусные и фитоплазменные болезни плодовых и ягодных культур в Беларуси. Минск, 2012.
3. Aramburu J., Rovira M. // J. of Horticult. Sci. and Biotechnol. 1998. Vol. 73. Р. 97-101.
4. Gottlieb A. R., Berbee J. G. // Phytopathology. 1973. Vol. 63. Р. 1470-1477.
5. Tzanetakis I. E., Martin R. R. // Plant Dis. 2005. Vol. 89. Р. 31.
6. Wong S. M., Horst R. K. // J. of Phytopathol. 1993. Vol. 139. Р. 33-47.
7. Certification schemes. Pathogen-tested material of Hop. EPPO Standards PM 4/16 (1) // Bull. OEPP/EPPO. 1998. Vol. 23. P. 735-736.
8. Postman J. D., Denoma J. S., Reed B. M. // Acta Horticulturae. 2005. Vol. 668. P. 143-147.
9. Choi S. H., Ryu K. H. // Plant Pathol. J. 2003. Vol. 19. P. 159-161.
10. Postman J. D., Cameron H. R. // Plant Dis. 1987. Vol. 71. P. 944-945.
11. Greber R. S., Klose M. J., Milne R. S., Teakle D. S. // Ann. Appl. Biol. 1991. Vol. 118. P. 589-593.
12. Singh R. P., Somerville T. H. // Plant Dis. 1983. Vol. 67. Р. 1133-1136.
13. Lister R. M., Rochow W. F. // Phytopathology. 1979. Vol. 69. Р. 649-654.
14. Rochow W. F. // Phytopathology. 1979. Vol. 69. Р. 655-660.
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