Phylogenetic relationship of apple stem grooving virus and its prevalence in the gardens of Belarus
Abstract
Apple trees of different cultivars growing in Belarus were tested using RT-PCR for Apple stem grooving virus, an important economically and common pathogens in commercial orchards. 115 apple trees and 64 raws of clonal rootstocks were tested. 9.6 % of apple trees were infected with ASGV. In 6.2 % of clonal rootstock were foud infected plants. Specificity of molecular markers was confirmed by sequencing the RT-PCR product. The nucleotide and deduced amino acid sequences were compared with sequences published in GenBank. Alignment this nucleotide and amino acid sequences of hypothetical protein sequence with other similar sequences from GenBank showed up to 99 and 100 % identity, respectively.
About the Authors
P. V. Kuzmitskaya
Институт генетики и цитологии НАН Беларуси
Belarus
Belarus
O. Yu. Urbanovich
Институт генетики и цитологии НАН Беларуси
Belarus
Belarus
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