Isolation of bacterial antagonistic strains of the genus Bacillus, with potential for the control of fish pathogenic microbiota
https://doi.org/10.29235/1029-8940-2026-71-2-125-131
Abstract
In recent years, the attention of researchers and practitioners has been focused on the use of probiotic drugs in aquaculture, which are characterized by high antimicrobial activity against target pathogens and have a pronounced stimulating effect on fish productivity. In order to develop such drugs, we screened isolates of spore-forming bacteria of the genus Bacillus isolated from the intestines of carp and waters of fish ponds. Two of the most active strains of antagonist bacteria were selected, and their morphological, physiological, and biochemical properties were studied. Amplification with specific primers revealed the presence of genes responsible for the synthesis of major antimicrobial metabolites.
About the Authors
E. A. Sambuk
State Scientific and Production Association “Chemical Synthesis and Biotechnology”
Belarus
Belarus
Ekaterina A. Sambuk – Scientific Researcher
2, Akademika Kuprevicha Str., office 7, 220084, Minsk
E. I. Kolomiets
State Scientific and Production Association “Chemical Synthesis and Biotechnology”
Belarus
Belarus
Emiliya I. Kolomiets ‒ Academician, D. Sc. (Biol.), Professor, General Director
2, Akademika Kuprevicha Str., office 7, 220084, Minsk
References
1. Maksim’yuk E. V., Poloz S. V., Degtyarik S. M., Slobodnitskaya G. V., Govor T. A., Bespalyi A. V. Methodological recommendations for reducing the damage caused to fish farming organizations by representatives of saprophytic and opportunistic microflora. Minsk, Information and Computing Center of the Ministry of Finance, 2024. 24 p. (in Russian).
2. Skogoreva A. M., Manzhurina O. A., Romashov B. V. Diagnosis of infectious fish diseases: a textbook. Voronezh, Voronezh State Agrarian University named after Emperor Peter the Great, 2016. 108 p. (in Russian).
3. Rakhkonen R., Vennerstrem P., Rintamyaki P., Kannel R. Finnish Research Institute of Hunting and Fisheries. Healthy fish. Prevention, diagnosis and treatment of diseases. 2nd ed. Helsinki, Finnish Game and Fisheries Institute, 2013. 177 p. (in Russian).
4. Sales of veterinary antimicrobial agents in 31 European countries in 2022 Trends from 2010 to 2022. European Medicines Agency. Avaliable at: https://www.ema.europa.eu/en/documents/report/sales-veterinary-antimicrobial-agents-31-european-countries-2022-trends-2010-2022-thirteenth-esvac-report_en.pdf (accessed 17.02.2026).
5. Shul’gina L. V., Yakush E. V., Shul’gin Yu. P., Shenderyuk V. V., Chukalova N. N., Bakholdina L. P. Antibiotics in aquaculture and their ecological significance. A review. Izvestiya TINRO = Russian Journal of Marine Biology, 2015, vol. 181, pp. 216–230 (in Russian).
6. Shchepetkina S. V. Organization of a system for the control of bacterial diseases, the use of antimicrobial drugs in animal husbandry and poultry farming to ensure the safety of products for the life and health of citizens. Infektsiya i immunitet [Infection and immunity], 2017, no. S, p. 978 (in Russian).
7. Tyurina D. G., Gorfunkel’ E. P., Filippova V. A., Laptev G. Yu., Novikova N. I., Iyldyrym E. A. [et al.]. The development of antimicrobial resistance in broilers affected by veterinary antimicrobials and a probiotic administration. Agrarnaya nauka = Agrarian science, 2024, vol. 380, no. 3, pp. 85–91 (in Russian).
8. Tekebaeva Zh. B., Shakhabaeva G. S., Sarmurzina Z. S., Bisenova G. N., Urazova M. S., Dosova A. D., Abzhalelov A. B. Probiotics and their use in aquaculture. Novosti nauki Kazakhstana [Science news from Kazakhstan], 2020, no. 4, pp. 170– 185 (in Russian).
9. Tekebaeva Zh. B., Kulagin A. A., Bisenova G. N., Beisenova R. R., Sarmurzina Z. S. Study of the role of probiotics against pathogenic bacteria of the genus Aeromonas and Pseudomonas in carp aquaculture in vitro. Problemy regional’noi ekologii [Problems of regional ecology], 2022, no. 1, pp. 16–23 (in Russian).
10. Lysak V. V., Zheldakova R. A. (auth. comp.). Microbiology: methodological recommendations for laboratory classes and control of students’ independent work. Minsk, Belarusian State University, 2002. 99 p. (in Russian).
11. Pimenova M. N., Grechushkina N. N., Netrusov A. I., Semenova E. V., Zakharchuk L. M., Zinchenko V. V., Kolotilova N. N., Myl’nikova S. I., Nefelova M. V., Botvinko I. V. A guide to practical classes in microbiology. 3nd ed. Moskow, Publishing house of Moscow University, 1995. 224 p. (in Russian).
12. Petrova I. S., Vintsyunaite M. M. Determination of proteolytic activity of enzyme preparations of microbiological origin. Prikladnaya biokhimiya i mikrobiologiya = Applied Biochemistry and Microbiology, 1980, vol. 2, no. 2, pp. 322–327 (in Russian).
Review
Views:
85
JATS XML
Email this article 
