Особенности структурно-функциональной организации генома бактерии Pseudomonas amygdali pv. lachrymans 8 – возбудителя угловатой пятнистости листьев огурца

В статье отражены результаты секвенирования, молекулярно-генетического и сравнительного анализа генома фитопатогенной бактерии Pseudomonas amygdali pv. lachrymans 8. Собранная геномная последовательность депонирована в базу данных GenBank Национального центра биотехнологической информации (НЦБИ) США (номера для доступа: CP075686–CP075690). В результате расчета средней нуклеотидной идентичности определено, что последовательность генома штамма 8 имеет сходство 99,87 % и 99,79 % с последовательностями геномов бактерий P. amygdali pv. lachrymans 814/98 и P. amygdali pv. lachrymans M301315 соответственно. Установлено, что геном штамма 8 представлен кольцевой хромосомой размером 6 054 652 п. н. с содержанием ГЦ-пар 58,11 % и четырьмя кольцевыми плазмидами: pPAL8-01 (77 748 п. н., содержание ГЦ-пар 56 %), pPAL8-02 (72 398 п. н., содержание ГЦ-пар 55 %), pPAL8-03 (49 000 п. н., содержание ГЦ-пар 54 %) и pPAL8-04 (9600 п. н., содержание ГЦ-пар 55 %). Высказано предположение, что путь распространения изучаемого фитопатогена осуществлялся либо параллельно из Нидерландов в США и Беларусь, либо сначала из Нидерландов на территорию США, а затем в Беларусь. Осуществлено сравнение нуклеотидной последовательности штамма 8 с нуклеотидными последовательностями бактерий P. amygdali pv. lachrymans M301315, NM002 и YM7902, в результате чего выявлены значительные генетические перестройки и определена локализация уникальной области размером 19 773 п. н.

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