Wolf-dog hybrids in Belarus: identification and rate estimation
https://doi.org/10.29235/1029-8940-2025-70-2-125-134
Abstract
In Belarus, the wolf is classified as an unregulated hunting species, and hunting is permitted throughout the year. For many years, the wolf population has been under hunting pressure, yet a moderate proportion persists. However, the disruption of the territorial and sexual structure of the wolf population in the context of a high numbers of stray dogs and free-ranging dogs, can lead to a number of negative consequences, one of which is hybridization. The paper presents the results of molecular genetic identification of wolf-dog hybrids based on STR analysis and the first data on the prevalence of such animals in Belarus. The obtained data confirm the presence of hybridization of wolves with stray dogs in Belarus, while no data are shown on any geographical conditionality or the concentration of hybrid individuals in the regions of the country.
Keywords
About the Authors
V. O. MolchanBelarus
Vladislav O. Molchan – Junior Researcher, Graduate Student
27, Akademicheskaya Str., 220072, Minsk
K. V. Homel
Belarus
Kanstantsin V. Homel ‒ Ph. D. (Biol.), Leading Researcher.
27, Akademicheskaya Str., 220072, Minsk
M. E. Nikiforov
Belarus
Michail E. Nikiforov – Academician, D. Sc. (Biol.), Professor, Head of the Laboratory
27, Akademicheskaya Str., 220072, Minsk
References
1. Ðan M., Šnjegota D., Veličković N., Stefanović M., Obreht Vidaković D., Ćirović D. Genetic variability and population structure of grey wolf (Canis lupus) in Serbia. Russian Journal of Genetics, 2016, vol. 52, no. 8, pp. 821–827. https://doi.org/10.1134/s1022795416080044
2. Vilà C., Wayne R. K. Hybridization between Wolves and Dogs. Conservation Biology, 1999, vol. 13, no. 1, pp. 195–198. https:// doi.org/10.1046/j.1523-1739.1999.97425.x
3. Dziech A. Identification of Wolf-Dog Hybrids in Europe – An Overview of Genetic Studies. Frontiers in Ecology and Evolution, 2021, vol. 9, art. 760160. https://doi.org/10.3389/fevo.2021.760160
4. Kays R., Curtis A., Kirchman J. J. Rapid adaptive evolution of northeastern coyotes via hybridization with wolves. Biology Letters, 2010, vol. 6, no. 1, pp. 89–93. https://doi.org/10.1098/rsbl.2009.0575
5. Rhymer J. M., Simberloff D. Extinction by hybridization and introgression. Annual Review of Ecology and Systematics, 1996, vol. 27, no. 1, pp. 83–109. https://doi.org/10.1146/annurev.ecolsys.27.1.83
6. Allendorf F. W., Leary R. F., Spruell P., Wenburg J. K. The problems with hybrids: setting conservation guidelines. Trends in Ecology & Evolution, 2001, vol. 16, no. 11, pp. 613–622. https://doi.org/10.1016/s0169-5347(01)02290-x
7. Garroway C. J., Bowman J., Cascaden T. J., Holloway G. L., Mahan C. G., Malcolm J. R., Steele M. A., Turner G., Wilson P. J. Climate change induced hybridization in flying squirrels. Global Change Biology, 2010, vol. 16, no. 1, pp. 113–121. https://doi.org/10.1111/j.1365-2486.2009.01948.x
8. Kidd A. G., Bowman J., Lesbarrères D., Schulte-Hostedde A. I. Hybridization between escaped domestic and wild American mink (Neovison vison). Molecular Ecology, 2009, vol. 18, no. 6, pp. 1175–1186. https://doi.org/10.1111/j.1365-294x.2009.04100.x
9. Mullen S. P., Little K., Draud M., Brozek J., Itzkowitz M. Hybridization among Caribbean damselfish species correlates with habitat degradation. Journal of Experimental Marine Biology and Ecology, 2012, vol. 416–417, pp. 221–229. https://doi.org/10.1016/j.jembe.2012.01.002
10. Rutledge L. Y., White B. N., Row J. R., Patterson B. R. Intense harvesting of eastern wolves facilitated hybridization with coyotes: Culling Facilitates Hybridization. Ecology and Evolution, 2011, vol. 2, no. 1, pp. 19–33. https://doi.org/10.1002/ece3.61
11. Randi E. Detecting hybridization between wild species and their domesticated relatives. Molecular Ecology, 2007, vol. 17, no. 1, pp. 285–293. https://doi.org/10.1111/j.1365-294x.2007.03417.x
12. Dolf G., Schläpfer J., Gaillard C., Randi E., Lucchini V., Breitenmoser U., Stahlberger-Saitbekova N. Differentiation of the Italian wolf and the domestic dog based on microsatellite analysis. Genetics Selection Evolution, vol. 32, art. 533. https://doi.org/10.1186/1297-9686-32-5-533
13. Smeds L., Aspi J., Berglund J., Kojola I., Tirronen K., Ellegren H. Whole‐genome analyses provide no evidence for dog introgression in Fennoscandian wolf populations. Evolutionary Applications, 2020, vol. 14, no. 3, pp. 721–734. https://doi.org/10.1111/eva.13151
14. Moura A. E., Tsingarska E., Dąbrowski M. J., Czarnomska S. D., Jędrzejewska B., Pilot M. Unregulated hunting and genetic recovery from a severe population decline: the cautionary case of Bulgarian wolves. Conservation Genetics, 2014, vol. 15, no. 2, pp. 405–417. https://doi.org/10.1007/s10592-013-0547-y
15. Randi E., Hulva P., Fabbri E., Galaverni M., Galov A., Kusak J., Bigi D., Černá Bolfíková B., Smetanová M., Caniglia R. Multilocus Detectionof Wolf x Dog Hybridization in Italy, and Guidelines for Marker Selection. PloS ONE, 2014, vol. 9, no. 1, p. e86409. https://doi.org/10.1371/journal.pone.0086409
16. Torres T. R., Ferreira E., Rocha R. G., Fonseca C. Hybridization between wolf and domestic dog: First evidence from an endangered population in central Portugal. Mammalian Biology, 2017, vol. 86, pp. 70–74. https://doi.org/10.1016/j.mambio.2017.05.001
17. Jarausch A., von Thaden A., Sin T., Corradini A., Pop M. I., Chiriac S., Gazzola A., Nowak C. Assessment of genetic diversity, population structure and wolf-dog ybridization in the Eastern Romanian Carpathian wolf population. Scientific Reports, 2023, vol. 13, no. 1, art. 22574. https://doi.org/10.1038/s41598-023-48741-x
18. Hindrikson M., Männil P., Ozolins J., Krzywinski A., Saarma U. Bucking the Trend in Wolf-Dog Hybridization: First Evidence from Europe of Hybridization between Female Dogs and Male Wolves. PloS ONE, 2012, vol. 7, no. 10, p. e46465. https://doi.org/10.1371/journal.pone.0046465
19. Andersone Ž., Lucchini V., Ozoliņš J. Hybridisation between wolves and dogs in Latvia as documented using mitochondrial and microsatellite DNA markers. Mammalian Biology, 2002, vol. 67, no. 2, pp. 79–90. https://doi.org/10.1078/1616-5047-00012
20. Korablev M. P., Korablev N. P., Korablev P. N. Genetic diversity and population structure of the grey wolf (Canis lupus Linnaeus, 1758) and evidence of wolf × dog hybridization in the centre of European Russia. Mammalian Biology, 2020, vol. 101, no. 1, pp. 91–104. https://doi.org/10.1007/s42991-020-00074-2
21. Stronen A. V., Jędrzejewska B., Pertoldi C., Demontis D., Randi E., Niedziałkowska M., Pilot M., Sidorovich V. E., Dykyy I., Kusak J., Tsingarska E., Kojola I., Karamanlidis A. A., Ornicans A., Lobkov V. A., Dumenko V., Czarnomska S. D. North-South Differentiation and a Region of High Diversity in European Wolves (Canis lupus). PloS ONE, 2013, vol. 8, no. 10, p. e76454. https://doi.org/10.1371/journal.pone.0076454
22. Molchan V. O. Registration of possible hybrids between the wolf (Canis lupus lupus) and the dog (Canis lupus familiaris) in Belarus. Molodezh’ v nauke – 2021: tezisy dokladov XVIII Mezhdunarodnoi nauchnoi konferentsii molodykh uchenykh (Minsk, 27– 30 sentyabrya 2021 goda) [Youth in Science – 2021: Abstracts of the XVIII International Scientific Conference of Young Scientists (Minsk, September 27–30, 2021)]. Minsk, 2021, vol. 1, pp. 256–258 (in Russian).
23. Sidorovich V., Rotenko I. Reproduction biology in grey wolves Canis lupus in Belarus: Common beliefs versus reality. 2nd ed. Minsk, Chatyry chverci, 2019. 224 p. (in Russian).
24. Korbie D. J., Mattick J. S. Touchdown PCR for increased specificity and sensitivity in PCR amplification. Nature Protocols, 2008, vol. 3, no. 9, pp. 1452–1456. https://doi.org/10.1038/nprot.2008.133
25. Matschiner M., Salzburger W. TANDEM: integrating automated allele binning into genetics and genomics workflows. Bioinformatics, 2009, vol. 25, no. 15, pp. 1982–1983. https://doi.org/10.1093/bioinformatics/btp303
26. Van Oosterhout C., Hutchinson W. F., Wills D. P. M., Shipley P. micro ‐ checker: software for identifying and correcting genotyping errors in microsatellite data. Molecular Ecology Notes, 2004, vol. 4, no. 3, pp. 535–538. https://doi.org/10.1111/j.14718286.2004.00684.x
27. Rousset F. genepop’007: a complete re‐implementation of the genepop software for Windows and Linux. Molecular Ecology Resources, 2008, vol. 8, no. 1, pp. 103–106. https://doi.org/10.1111/j.1471-8286.2007.01931.x
28. Peakall R., Smouse P. E. genalex 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes, 2006, vol. 6, no. 1, pp. 288–295. https://doi.org/10.1111/j.1471-8286.2005.01155.x
29. Wringe B. F., Stanley R. R. E., Jeffery N. W., Anderson E. C., Bradbury I. R. parallelnewhybrid: an R package for the parallelization of hybrid detection using newhybrids. Molecular Ecology Resources, 2017, vol. 17, no. 1 (spec. iss.), pp. 91–95. https://doi.org/10.1111/1755-0998.12597
30. Pritchard J. K., Stephens M., Donnelly P. Inference of Population Structure Using Multilocus Genotype Data. Genetics, 2000, vol. 155, no. 2, pp. 945–959. https://doi.org/10.1093/genetics/155.2.945
31. Clumpak – Cluster Markov Packager Across K. Plant Evolution, bioinformatics, & comparative genomics. Available at: https://clumpak.tau.ac.il (accessed 01.12.2024).
32. Molchan V., Homel K., Valnisty A., Nikiforov M., Kheidorova E. Genetic diversity of mtDNA in the grey wolf population of Belarus threatened by wolf–dog admixture. Theriologia Ukrainica, 2023, vol. 25, pp. 87–99. https://doi.org/10.53452/tu2508