Establishing the breed composition of pigs using KASP technology

A high differentiating potential of seven polymorphic variants has been identified to distinguish between Yorkshire, Belarusian Large White and Belarusian Meat pig breeds bred in Belarus. For the Yorkshire pig breed, a model is proposed including three single nucleotide polymorphisms – Chr.6:g.121005974A>G, Chr.17:g.15827832G>T, Chr.10:g.30081932A>G; for the Belarusian Large White pig breed – Chr.6:g.121005974A>G, Chr.3:g.118879246C>G, Chr.7:g.52269732A>G; for the Belarusian Meat pig breed – Chr.8:g.47482649G>T, Chr.9:g.48882095A>G, Chr.10:g.30081932A>G. The models demonstrate high accuracy, specificity, and sensitivity values. The approach to differentiating pig breeds is based on the technology of сompetitive allele-specific PCR.

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