Molecular-genetic structure of Pseudomonas phage BIM BV-45 D
https://doi.org/10.29235/1029-8940-2022-67-2-190-196
Abstract
Full nucleotide sequence of bacteriophage Pseudomonas phage BIM BV-45 D – active component of biopesticide Multiphage intended for control of crop bacterial diseases caused by Pseudomonas syringae was analyzed. It was found that phage genome is represented by linear double-stranded DNA sized 40383 b. p. (average GC contents equals 58 %), comprising 46 open reading frames, including 13 described in genomes of closely related phages. 4 control sequences typical for bacterial genes recognized by sigma factor (σ70) of RNA polymerase were detected, specific phage promoters were not localized. The identity was established of the majority of protein amino acid sequences of the Pseudomonas phage BIM BV-45 D with the proteins of the known Pseudomonas phage Andromeda (95‒100 %), at the same time, the sequences of the DNA endonuclease protein (gene 22) are similar (63 %) to the corresponding protein of Pseudomonas phage PollyC. The obtained data suggest that the mosaic structure of Pseudomonas phage BIM BV-45 D genome is due to recombinant rearrangements between the afore-mentioned phages.
Keywords
genome,
bacteriophage,
next generation sequencing,
phytopathogenic bacteria Pseudomonas syringae,
biopesticide “Multiphage”
Supporting agencies: This work was supported by the Belarusian Republican Foundation for Fundamental Research (grant B 20R-078). We express sincere gratitude for assistance our studies provided by Ph. D. (Biol.) Leonid Valentovich
About the Authors
T. A. Pilipchuk
Institute of Microbiology, National Academy of Sciences of Belarus
Belarus
Belarus
Tatsiana A. Pilipchuk – Researcher.
2, Kuprevich Str., 2220141, Minsk
A. E. Akhremchuk
Institute of Microbiology, National Academy of Sciences of Belarus
Belarus
Belarus
Artur E. Akhremchuk – Junior Researcher.
2, Kuprevich Str., 2220141, Minsk
E. I. Kаlаmiуets
Institute of Microbiology, National Academy of Sciences of Belarus
Belarus
Belarus
Emiliya I. Kаlаmiуets – Academician, Dr. Sc. (Biol.), Professor, Chief Researcher.
2, Kuprevich Str., 2220141, Minsk
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