Highbush blueberry (Vaccinium corymbosum L.): proteomic and molecular genetic studies. Vestsi Natsyyanal’nai akademii navuk Belarusi

The Vacciniaceae family introduction carry out by the Central Botanical Garden of the National Academy of Sciences of Belarus since 1980. It made it possible to give rise the new direction for the republic – the industrial berry growing. To increase the efficiency of high-quality planting material obtaining it is necessary to study the fundamental processes underlying the growth and development of plants and to establish the possible ways of their regulation. It is of current interest to confirm the conformity of the planting material to the declared variety (the genetic passports elaboration). The biotechnology development promotes a new approaches and technologies for studying and analyzing of a plant organism genome and proteome. For the first time the molecular genetic identification for Vaccinium corymbosum L. varieties were carried out by Start Codon Targeted (SCoT) system. This method of DNA certification makes it possible to check the cultivar conformity of the blueberry planting material. For the first time by 2D electrophoresis the proteomic maps of leaf tissue were obtained and the proteomic status of four highbush blueberry varieties – in vitro culture and parental plants (in vivo) was estimated. The species and varieties specificity of the expressed genome products were established. The proteins that claims to be markers for the Vaccinium species and the variety markers were identified for the first time, as well as the physiological state markers of high blueberry cultivated in vivo and in vitro.

1. Phuong H. L., Thao T. D. Blueberry supplementation in neuronal health and protective technologies for efficient delivery of blueberry anthocyanins. Biomolecules, 2021, vol. 11, no. 1, art. 102. https://doi.org/10.3390/biom11010102

2. Rupasova Zh. A., Reshetnikov V. N., Ruban N. N. Ignatenko V. A., Yakovlev A. P., Pyatnitsa F. S. Highbush blueberry: assessment of adaptive potential during introduction in Belarus. Minsk, Belorusskaya nauka Publ., 2007. 442 p. (in Russian).

3. Titok V. V., Reshetnikov V. N., Volod’ko I. K., Pavlovskii N. B. History and results of the introduction of berry plants of the Ericaceae family in the Republic of Belarus. Opyt i perspektivy vyrashchivaniya netraditsionnykh yagodnykh rastenii na territorii Belarusi i sopredel’nykh stran : materialy Mezhdunarodnogo nauchno-prakticheskogo seminara (Minsk– Gantsevichi, 28 sentyabrya–01 oktyabrya 2021 goda) [Experience and prospects for growing non-traditional berry plants in Belarus and neighboring countries : materials of the International scientific and practical seminar (Minsk–Gantsevichi, September 28–October 01, 2021)]. Minsk, 2021, pp. 3–14 (in Russian).

4. Dempster E. L., Pryor K. V., Francis D., Young J. E., Rogers H. J. Rapid DNA extraction from ferns for PCR-based analyses. Biotechniques, 1999, vol. 27, no. 1, pp. 66–68. https://doi.org/10.2144/99271bm13

5. Collard B. C. Y., Mackill D. J. Start codon targeted (SCoT) polymorphism: a simple, novel DNA marker technique for generating gene-targeted markers in plants. Plant Molecular Biology Reporter, 2009, vol. 27, art. 86. https://doi.org/10.1007/s11105-008-0060-5

6. Mahjbi A., Baraket G., Oueslati A., Salhi-Hannachi A. Start Codon Targeted (SCoT) markers provide new insights into the genetic diversity analysis and characterization of Tunisian Citrus species. Biochemical Systematics and Ecology, 2015, vol. 61, pp. 390–398. https://doi.org/10.1016/j.bse.2015.07.017

7. Guo D. L., Zhang J. Y., Liu C. H. Genetic diversity in some grape varieties revealed by SCoT analyses. Molecular Biology Reports, 2012, vol. 39, no. 5, pp. 5307–5313. https://doi.org/10.1007/s11033-011-1329-6

8. Amme S., Rutten T., Melzer M., Sonsmann G., Vissers J., Schlesier B., Mock H.-P. A proteome approach defines protective functions of tobacco leaf trichomes. Proteomics, 2005, vol. 5, no. 10, pp. 2508–2518. https://doi.org/10.1002/pmic.200401274

9. Laemmli U. K. Cleavage of structural proteins during the assemly of the head of bacteriophage T4. Nature, 1970, vol. 227, no. 5259, pp. 680–685. https://doi.org/10.1038/227680a0

10. Prevost A., Wilkinson M. J. A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theoretical and Applied Genetics, 1999, vol. 98, no. 1, pp. 107–112. https://doi.org/10.1007/s001220051046

11. Nagy S., Poczai P., Cernák I., Gorji A. M., Hegedűs G., Taller J. PICcalc: an online program to calculate polymorphic information content for molecular genetic studies. Biochemical Genetics, 2012, vol. 50, pp. 670–672. https://doi.org/10.1007/s10528-012-9509-1

12. Jarillo J. A., Piñeiro M., Cubas P., Martínez-Zapater J. M. Chromatin remodeling in plant development. International Journal of Developmental Biology, 2009, vol. 53, pp. 1581–1596. https://doi.org/10.1387/ijdb.072460jj

13. Shemarova I. V. Post-translational regulation of programmed cellular processes in lower eukaryotes. Tsitologiya = Cell and Tissue Biology, 2008, vol. 50, no. 8, pp. 663–670 (in Russian).

14. Kuzovkova A. A., Spiridovich E. V., Reshetnikov V. N. Primary proteomic analysis of winter rye chloroplasts and methodological approaches to its implementation. Fiziologiya i biokhimiya kul’turnykh rastenii [Physiology and biochemistry of cultivated plants], 2011, vol. 43, no. 2, pp. 105–112 (in Russian).