Cryptic species of the genus Microtus on the territory of Belarus: taxonomic position and distribution
https://doi.org/10.29235/1029-8940-2021-66-4-475-481
Abstract
The paper presents the original data of the chromosomal and molecular-genetic (by PCR typing) analysis of cryptic species of the genus Microtus on the territory of Belarus. The aim of the study is to perform chromosomal labeling of common vole populations in various localities in Belarus to identify Microtus arvalis s. l. twin species in natural ecosystems. Based on the karyological analysis, 2 representatives of the cryptic group were identified in the study area: the Eastern European vole (2р = 54, NF = 56) and the common vole (2р = 46, NF = 86). Also, to confirm the species identity of the twin species, a molecular genetic analysis was performed by PCR typing. It was established that one studied individual from the cryptic group Microtus arvalis s. l. belongs to the species Microtus rossiaemeridionalis. A fragment of the cyt b mitochondrial gene was amplified in the size of 469 bp in the remaining individuals (n = 105), and a fragment of the cytochrome b mitochondrial gene in the size of 842 was amplified, which indicates that these representatives belong to the Microtus arvalis form “arvalis”. In conclusion, the results of the work done are summed up, and tasks are set for further research of cryptic species of the genus Microtus in Belarus.
About the Authors
Ya. I. Mashkou
Scientific and Practical Center of the National Academy of Sciences of Belarus for Bioresources
Belarus
Belarus
Yauheni I. Mashkou ‒ Postgraduate student, Junior
Researcher
27, Akademicheskaya Str., 220072, Minsk
H. S. Gajduchenko
Scientific and Practical Center of the National Academy of Sciences of Belarus for Bioresources
Belarus
Belarus
Helen S. Gajduchenko ‒ Ph. D. ( Biol.), L eading R esearcher
27, Akademicheskaya Str., 220072, Minsk
References
1. Manokhina N. V., Terekhovich V. F. Chromosomal set and taxonomic position of the common vole of Belarus. Vestnik Belorusskogo universiteta. Seriya 2. Khimiya. Biologiya. Geografiya [Bulletin of the Belarusian University. Series 2. Chemistry. Biology. Geography], 1979, vol. 2, no. 3, pp. 67–68 (in Russian).
2. Manokhina N. V. Ecological and morphological characteristics of the common vole of Belarus. Minsk, Institute of Zoology of the Academy of Sciences of the BSSR, 1981. 17 p. (in Russian).
3. Terekhovich V. F., Manokhina N. V. The taxonomic position and distribution of the common vole on the territory of Belarus. Mammals of the USSR. Mlekopitayushchie SSSR : tezisy dokladov III s’’ezda Vsesoyuznogo teriologicheskogo obshchestva (Moskva, 1–5 fevralya 1982 goda). Tom 1 [Mammals of the USSR : abstracts of the III congress of the All-Union theriological society (Moscow, February 1–5, 1982). Volume 1]. Moscow, 1982, p. 82 (in Russian).
4. Mashkov E. I., Gaiduchenko E. S., Krishchuk I. A., Solovei I. A. Estimation of the population density of Microtus species of the valley ecosystems of Belarus. Vesnіk Magіleuskaga dzyarzhaunaga unіversіteta іmya A. A. Kulyashova. Seryya B. Pryrodaznauchyya navukі: matematyka, fіzіka, bіyalogіya [Bulletin of Mogilev State University named after A. A. Kuleshov. Series B. Natural sciences: mathematics, physics, biology], 2019, pp. 96–109 (in Belarusian).
5. Mashkov E. I., Gaiduchenko E. S., Krishchuk I. A. Evaluation of the population density of species of the genus Microtus of the valley ecosystems of Belarus. Vestnik Grodnenskogo gosudarstvennogo universiteta imeni Yanki Kupaly. Seriya 5. Ekonomika. Sotsiologiya. Biologiya [Bulletin of the Yanka Kupala State University of Grodno. Series 5. Economy. Sociology. Biology], 2020, vol. 10, no. 2, pp. 138–147 (in Belarusian).
6. Naumov N. P. Essays on the comparative ecology of mouse rodents. Moscow, Leningrad, USSR Academy of Sciences Publ., 1948. 204 p. (in Russian).
7. Mikholap O. N., Mikhailovskaya V. V. Change in the number of red and common voles under the influence of land reclamation of the lowland swamp. Itogi issledovanii po mezhdunarodnoi biologicheskoi programme v BSSR : materialy Ob’’edinennoi sessii Otdeleniya biologicheskikh nauk AN BSSR i Belorusskogo respublikanskogo komiteta po MBP [Results of research on the International biological program in the BSSR : materials of the Joint session of the Department of Biological Sciences of the Academy of Sciences of the BSSR and the Belarusian Republican Committee on IBP]. Minsk, 1974, pp. 18–20 (in Russian).
8. Teslenko S. V., Zagorodnyuk I. V. Twin species of the Microtus arvalis overspecies in Ukraine. Vesnik zoologii [Bulletin of zoology], 1986, no. 6, pp. 27–31 (in Russian).
9. Ford C. E., Hamerton J. L. A colchicines hypotonic citrate squash. Stain Technology, 1956, vol. 31, no. 6, pp. 247–251. https://doi.org/10.3109/10520295609113814
10. Baskevich M. I., Potapov S. G., Okulova N. M., Capel’nikov S. F., Vlasov A. A., Oparin M. L., Mironova T. A., Avilova E. A. On the spread of variability of twin species Microtus arvalis s. l. (Rodentia, Arvicolinae) in the Central Chernozem region according to chromosomal and molecular genetic data. Zoologicheskii zhurnal [Zoological journal], 2009, vol. 88, no. 4, pp. 473–487 (in Russian).
11. Potapov S. G., Okulova N. M., Baskevich M. I. Molecular genetic studies (RAPD analysis) of Microtus arvalis sensu lato (Rodentia, Arvicolidae) on the Russian Plain. Molekulyarno-geneticheskie osnovy sokhraneniya bioraznoobraziya mlekopitayushchikh Golarktiki : materialy Mezhdunarodnoi konferentsii (Chernogolovka, 26‒30 noyabrya 2007 goda) [Molecular genetic basis for the conservation of biodiversity of mammals in the Holarctic : materials of the International conference (Chernogolovka, November 26‒30, 2007)]. Moscow, 2007, pp. 213–220 (in Russian).
12. Mashkov E. I. Analysis of the karyotype of the common vole inhabiting natural ecosystems of Belarus. Struktura i dinamika bioraznoobraziya : materialy I Respublikanskoi zaochnoi nauchno-prakticheskoi konferentsii molodykh uchenykh (Minsk, 23 dekabrya 2019 goda) [The structure and dynamics of biodiversity : materials of the I Republican correspondence scientific and practical conference of young scientists (Minsk, December 23, 2019)]. Minsk, 2019, pp. 52–54 (in Russian).
13. Shenbrot G. I., Krasnov B. R Atlas of the Geographic Distribution of the Arvicoline Rodents of the World (Rodentia, Muridae: Arvicolinae). Sofia, Pensoft Publ., 2005. 336 p.
Review
Views: 721
Email this article 
