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Apple gene MD13G1109800 is a member of Trihelix family transcription factors and expressed in response to abiotic stress

https://doi.org/10.29235/1029-8940-2021-66-4-426-432

Abstract

The Trihelix family of transcription factors plays an important role in the plant’s response to various abiotic stress types. In this work in apple Golden Delicious genome we identified apple gene MD13G1109800 as a member of Triheilx family in silico. Analysis of chromosomal localization showed that it is located on chromosome 13 and has four introns. The hypothetical protein encoded by it has a length of 365 amino acid residues, a molecular weight of 42097.23 Da, an isoelectric point pI = 6.21 and located in the nucleus. Analysis of the promoter region of the MD13G1109800 gene indicates that its product is a member of many signaling pathways triggered by both external and internal factors. The expression level of the MD13G1109800 gene increases under drought, low and high temperatures, as well as salinity in the MM-106 apple rootstock.

About the Authors

P. V. Kuzmitskaya
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus,
Belarus

Polina V. Kuzmitskaya – P h. D . ( Biol.), S enior R esearcher

27, Akademicheskaya Str., 220072, Minsk



K. S. Karaleva
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus,
Belarus

Katsiaryna S. Karaleva – Junior Researcher

27, Akademicheskaya Str., 220072, Minsk



O. Yu. Urbanovich
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus,
Belarus

Oksana Yu. Urbanovich – D. Sc. (Biol.), Associate Professor, Head of the Laboratory

27, Akademicheskaya Str., 220072, Minsk



References

1. Ingram J., Bartels D. The molecular basis of dehydration tolerance in plants. Annual Review of Plant Physiology and Plant Molecular Biology, 1996, vol. 47, no. 1, pp. 377–403. https://doi.org/10.1146/annurev.arplant.47.1.377

2. Shinozaki K., Yamaguchi-Shinozaki K. Gene expression and signal transduction in water-stress response. Plant Physiology, 1997, vol. 115, no. 2, pp. 327–334. https://doi.org/10.1104/pp.115.2.327

3. Shinozaki K., Yamaguchi-Shinozaki K. Molecular responses to dehydration and low temperature: differences and cross-talk between two stress signaling pathways. Current Opinion in Plant Biology, 2000, vol. 3, no. 3, pp. 217–223. https://doi.org/10.1016/s1369-5266(00)80068-0

4. Li J., Zhang M., Sun J., Mao X., Wang J., Wang J. [et al.]. Genome-wide characterization and identification of trihelix transcription factor and expression profiling in response to abiotic stresses in rice (Oryza sativa L.). International Journal of Molecular Sciences, 2019, vol. 20, no. 3, p. 251. https://doi.org/10.3390/ijms20020251

5. Murata J., Takase H., Hiratsuka K. Characterization of a novel GT-box binding protein from Arabidopsis. Plant Biotechnology, 2002, vol. 19, no. 2, pp. 103–112. https://doi.org/10.5511/plantbiotechnology.19.103

6. Xie Z.-M., Zou H.-F., Lei G., Wei W., Zhou Q.-Y., Niu C.-F. [et al.]. Soybean trihelix transcription factors GmGT-2A and GmGT-2B improve plant tolerance to abiotic stresses in transgenic Arabidopsis. PLoS ONE, 2009, vol. 4, no. 9, p. e6898. https://doi.org/10.1371/journal.pone.0006898

7. Gao M. J., Lydiate D. J., Li X., Lui H., Gjetvaj B., Hegedus D. D. [et al.]. Repression of seed maturation genes by a trihelix transcriptional repressor in Arabidopsis seedlings. Plant Cell, 2009, vol. 21, no. 1, pp. 54–71. https://doi.org/10.1105/tpc.108.061309

8. Yu C., Cai X., Ye Z., Li H. Genome-wide identification and expression profiling analysis of trihelix gene family in tomato. Biochemical and Biophysical Research Communications, 2015, vol. 468, no. 4, pp. 653–659. https://doi.org/10.1016/j.bbrc.2015.11.010

9. El-Gebali S., Mistry J., Bateman A., Eddy S. R., Luciani A., Potter S. C. [et al.]. The Pfam protein families database in 2019. Nucleic Acids Research, 2018, vol. 47, no. D1, pp. D427–D432. https://doi.org/10.1093/nar/gky995

10. Jaakola L., Pirttilä A. M., Halonen M., Hohtola A. Isolation of high quality RNA from bilberry (Vaccinium myrtillus L.) fruit. Molecular Biotechnology, 2001, vol. 19, no. 2, pp. 201–203. https://doi.org/10.1385/MB:19:2:201

11. Bustin S. A., Benes V., Garson J. A., Hellemans J., Huggett J., Kubista M. [et al.]. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Oxford University Press, 2009, vol. 55, no. 4, pp. 611–622. https://doi.org/10.1373/clinchem.2008.112797

12. Zhao T., Liang D., Wang P., Liu J., Ma F. Genome-wide analysis and expression profiling of the DREB transcription factor gene family in Malus under abiotic stress. Molecular Genetics and Genomics, 2012, vol. 287, no. 5, pp. 423–436. https://doi.org/10.1007/s00438-012-0687-7

13. Rao X., Huang X., Zhou Z., Lin X. An improvement of the 2ˆ (–delta delta CT) method for quantitative real-time polymerase chain reaction data analysis. Biostatistics, Bioinformatics and Biomathematics, 2013, vol. 3, no. 3, pp. 71–85.

14. Meng D., Li Y., Bai Y., Li M., Cheng L. Genome-wide identification and characterization of WRKY transcriptional factor family in apple and analysis of their responses to waterlogging and drought stress. Plant Physiology and Biochemistry, 2016, vol. 103, pp. 71–83. https://doi.org/10.1016/j.plaphy.2016.02.006

15. Li T., Xu Y., Zhang L., Ji Y., Tan D., Yuan H. [et al.]. The jasmonate-activated transcription factor MdMYC2 regulates ethylene response factor and ethylene biosynthetic genes to promote ethylene biosynthesis during apple fruit ripening. Plant Cell, 2017, vol. 29, no. 6, pp. 1316–1334. https://doi.org/10.1105/tpc.17.00349

16. Gao H., Huang R., Liu J., Gao Z., Zhao H., Li X. Genome-wide identification of trihelix genes in moso bamboo (Phyllostachys edulis) and their expression in response to abiotic stress. Journal of Plant Growth Regulation, 2019, vol. 38, pp. 1127–1140. https://doi.org/10.1007/s00344-019-09918-9


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ISSN 1029-8940 (Print)
ISSN 2524-230X (Online)