Tissue dermal equivalent – а cellular product based on human dermal keratinocytes and fibroblasts: the properties of equivalent components and perspectives of practical application
https://doi.org/10.29235/1029-8940-2021-66-4-391-401
Abstract
The nature and ways of isolation and cultivation in vitro of keratinocytes and fibroblasts, the main cellular components of skin to prepare a new biomedical product, tissue dermal equivalent were considered. The main attention was payed to optimization of upbuilding dermal cell biomass including selection of medium compositions and conditions of cultivation. The information was given on main parameters of cell cultures as proliferation activity, viability and phenotype of the cells. Genotoxicity of fibroblasts and biocompatibility of the cells with organic matrixes to find the optimal carrier for cellular elements of tissue dermal equivalent were studied. The composition, the process of preparation of tissue dermal equivalent and perspectives of its practical application were discussed.
Keywords
About the Authors
A. V. Butenka
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Anna V. Butenka – Junior Researcher
27, Akademicheskaya Str., 220072, Minsk
Z. B. Kvacheva
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Zinaida B. Kvacheva – Ph. D. (Biol.), Leading Researcher
27, Akademicheskaya Str., 220072, Minsk
I. B. Vasilevich
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Irina B. Vasilevich – Researcher
27, Akademicheskaya Str., 220072, Minsk
A. Ch. Chasnoit
Belarusian Medical Academy of Postgraduate Education
Belarus
Belarus
Alexej Ch. Chasnoit – Ph. D. (Med.), Associate Professor
3/3, P. Browka Str., 220013, Minsk
I. D. Volotovski
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Igor D. Volotovski – Academician, D. S c. ( Biol.), Professor, Chief Researcher
27, Akademicheskaya Str., 220072, Minsk
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