Lithium-induced modiphication of the physicochemical state of membrane proteins and lipids in human erythrocytes
https://doi.org/10.29235/1029-8940-2021-66-3-295-301
Abstract
The effect of various concentrations of lithium sulfate on human erythrocytes in vitro has been studied. It has been shown that the effect of lithium salt in maximum pharmacological and toxic concentrations on cells leads to a modification of the physicochemical state of membrane-bound proteins and lipids. It was found that in human erythrocytes exposed to lithium ions, there is a decrease in the activity of membrane-bound acetylcholinesterase and methgemoglobin reductase, as well as a change in the microviscosity of the lipid bilayer of membranes. The results obtained can be used to create a cell test system for assessing the toxicity of lithium compounds.
Keywords
erythrocyte membrane,
lithium sulfate,
lipophilic probes,
acetylcholinesterase,
methgemoglobin reductase,
lipid microviscosity
Supporting agencies: The work was funded within the framework of the State Program of Scientific Research of the Republic of Belarus “Biotechnologies” (grant 1.52, 2019-2020).
About the Authors
G. P. Zubritskaya
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Galina P. Zubritskaya - Ph. D. (Biol.), Leading Researcher, Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus.
27, Akademicheskaya Str., 220072, Minsk.
E. I. Slobozhanina
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Ekaterina I. Slobozhanina - Corresponding Member, D. Sc. (Biol.), Professor, Head of the Laboratory, Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus.
27, Akademicheskaya Str., 220072, Minsk.
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