Evaluation of the genetic variability of Ophrys insectifera L. populations growing in Belarus using molecular iPBS markers
https://doi.org/10.29235/1029-8940-2021-66-2-223-231
Abstract
An analysis of genetic heterogeneity was performed for two populations of Ophrys insectifera L. located in the Berezinsky Biosphere Reserve and the Verkudy village. The species are critically endangered (CR). The analysis of data obtained using iPBS markers detected the higher risk of extinction of the population in the Berezinsky Biosphere Reserve than the population from the Verkudy village, since the level of population genetic heterogeneity from the Verkudy is higher compared to the Berezinsky Biosphere Reserve. Molecular variance analysis (AMOVA) and other parameters of genetic variation showed the major fraction of intrapopulation variation (64 %, Gst = 0.26). Principal Coordinates Analysis (PCoA) revealed two groups that coincided with natural populations, and confirmed a larger genetic heterogeneity in the population from the Verkudy.
About the Authors
N. V. Samokhvalova
Central Botanical Garden of the National Academy of Sciences of Belarus
Belarus
Belarus
Natalia V. Samokhvalova, Junior Reseаrcher
2v, Surganov Str., 220012, Minsk
A. V. Kruchonok
Central Botanical Garden of the National Academy of Sciences of Belarus
Belarus
Belarus
Alesia V. Kruchonok, Reseаrcher, Sector Manager
2v, Surganov Str., 220012, Minsk
B. Yu. Anoshenko
Central Botanical Garden of the National Academy of Sciences of Belarus
Belarus
Belarus
Boris Yu. Anoshenko, Ph. D. (Biol.), Head of the Laboratory
2v, Surganov Str., 220012, Minsk
V. V. Titok
Central Botanical Garden of the National Academy of Sciences of Belarus
Belarus
Belarus
Vladimir V. Titok, Corresponding Member, D. Sc. (Biol.), Associate Professor, Director
2v, Surganov Str., 220012, Minsk
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