Study of the gene pool of the winter rye Secale cereale L. of the Republic of Belarus using microsatellite markers
https://doi.org/10.29235/1029-8940-2021-66-2-215-222
Abstract
In this work, the genetic diversity of the modern gene pool of the winter rye (S. cereal L.) of the Republic of Belarus from 20 actual breeding samples was investigated using 15 microsatellite (SSR) markers to develop divergent crossing combinations in breeding for heterosis. It was shown that the formed set of SSR markers is highly effective – the informational content index (PIC) varied from 0.50 to 0.83 and averaged 0.72. The most effective microsatellite markers (SCM28, SCM43, SCM101 and SCM102) were identified and can be successfully used to study the genetic diversity of rye. It has been established that the modern gene pool of the winter rye of the Republic of Belarus is generally characterized by fairly wide genetic diversity (interpopulation variability) – all collection samples are characterized by a unique allelic composition of the studied microsatellite loci. Based on investigation results, a hierarchical clustering dendrogram was constructed, which made it possible to determine the most genetically divergent combinations of crosses. The information obtained can be used for the development of an effective scheme allowing to develop new varieties and hybrids in the practical breeding of rye for heterosis.
About the Authors
V. S. Mandrusova
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Victoria S. Mandrusova, Junior Researcher
27, Akademicheskaya Str., 220072
I. S. Gordej
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Igor S. Gordej, Ph. D. (Biol.), Senior Researcher
27, Akademicheskaya Str., 220072
O. M. Lyusikov
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Oleg M. Lyusikov, Researcher
27, Akademicheskaya Str., 220072
V. E. Shimko
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Victoria E. Shimko, Researcher
27, Akademicheskaya Str., 220072
I. A. Gordej
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Belarus
Ivan A. Gordej, D. Sc. (Biol.), Professor, Chief Researcher
27, Akademicheskaya Str., 220072
References
1. Urban E. P. Winter rye in Belarus: selection, seed production, cultivation technology. Minsk, Belaruskaya navuka Publ., 2009. 269 p. (in Russian).
2. Kobylyanskii V. D., Solodukhina O. V. The theoretical basis of grain fodder rye breeding for low water soluble pentosans. Sel’skokhozyaistvennaya biologiya [Agricultural biology], 2013, no. 2, pp. 31–39 (in Russian).
3. Goncharenko A. A. New directions in selection of a winter rye on target use. Zernobobovye i krupyanye kul’tury [Legumes and groat crops], 2016, vol. 2, no. 18, pp. 25–32 (in Russian).
4. Privalov F. I., Urban E. P. Achievements and problems of high yield crops breeding in the Republic of Belarus. Vestsі Natsyyanal’nai akademіі navuk Belarusi. Seryya agrarnykh navuk = Proceedings of the National Academy of Sciences of Belarus. Agrarian series, 2016, no. 3, pp. 41–49 (in Russian).
5. Shlegel’ R. Hybrid breeding boosted molecular genetics in rye. Vavilovskіi zhurnal genetiki i selektsii = Vavilov Journal of Genetics and Breeding, 2015, vol. 19, no. 5, pp. 589–603 (in Russian).
6. Leonova I. N. Molecular markers: implementation in crop plant breeding for identification, introgression, and gene pyramiding. Vavilovskii zhurnal genetiki i selektsii = Vavilov Journal of Genetics and Breeding, 2013, vol. 17, no. 2, pp. 314– 325 (in Russian).
7. Chikmawati T., Miftahudin J., Gustafson J. P. Rye (Secale cereale L.) and wheat (Triticum aestivum L.) simple sequence repeat variation within Secale spp. (Poaceae). HAYATI Journal of Biosciences, 2013, vol. 20, no. 4, pp. 163–170. https://doi.org/10.4308/hjb.20.4.163
8. Adonina I. G., Leonova I. N., Badaeva E. D., Salina E. A. Genotyping of hexaploid wheat varieties from different Russian regions. Vavilovskii zhurnal genetiki i selektsii = Vavilov Journal of Genetics and Breeding, 2016, vol. 20, no. 1, pp. 44–50 (in Russian).
9. Shapturenko M. N., Pechkovskaya T. V., Vakula S. I., Yakimovich A. V., Zabara Yu. M., Khotyleva L. V. Informative EST-SSR markers for genotyping and intraspecific differentiation of Brassica oleracea var. capitata L. Vavilovskii zhurnal genetiki i selektsii = Vavilov Journal of Genetics and Breeding, 2016, vol. 20, no. 1, pp. 51–56 (in Russian).
10. Shang H.-I., Wei Y.-M., Wang X.-R., Zheng Y.-L. Genetic diversity and phylogenetic relationships in the rye genus Secale L. (rye) based on Secale cereale microsatellite markers. Genetics and Molecular Biology, 2006, vol. 29, no. 4, pp. 685– 691. https://doi.org/10.1590/S1415-47572006000400018
11. Targońska M., Bolibok-Brągoszewska H., Rakoczy-Trojanowska M. Assessment of genetic diversity in secale cereale based on SSR markers. Plant Molecular Biology Reporter, 2016, vol. 34, no. 1, pp. 37–51. https://doi.org/10.1007/s11105-015-0896-4
12. Saal B., Wricke G. Development of simple sequence repeat markers in rye (Secale cereale L.). Genome, 1999, vol. 42, no. 5, pp. 964–972. https://doi.org/10.1139/g99-052
13. Padutov V. E., Baranov O. Yu., Voropaev E. V. Molecular genetic analysis methods. Minsk, Yunipol Publ., 2007. 176 p. (in Russian).
14. Chesnokov Yu. V., Artem’eva A. M. Evaluation of the measure of polymorphism information of genetic diversity. Sel’skokhozyaistvennaya biologiya = Agricultural biology, 2015, vol. 50, no. 5, pp. 571–578 (in Russian).
15. Perrier X., Jacquemoud-Collet J. P. Darwin software (2006). Available at: http://darwin.cirad.fr/darwin (accessed 19.02.2020).
Review
Views: 496
Email this article 
