The identification and molecular characteristics of Belarusian apple proliferation phytoplasma isolates
https://doi.org/10.29235/1029-8940-2021-66-1-88-97
Abstract
It is known that optimal source of samples for diagnostic of apple proliferation phytoplasma in autumn is roots. In case of the occurrence of pronounced characteristic symptoms - “witches’ broom”, can be used symptomatic shoots.
Real-time PCR with primer pair Phyto-F/Phyto-R and probe Phyto-P and nested PCR with primer pair P1/Tint and fO1/ rO1 can be used to detect Candidatus Phytoplasma mali with a high degree of sensitivity and reproducibility.
Comparison of the nucleotide sequences of Belarusian isolates with the sequences presented in EMBL/GenBank showed that all Belarusian phytoplasma isolates detected on apple cultivars Alesya, Syabryna, Pamyat Sikory belong to species Candidatus Phytoplasma mali. The nucleotide sequences are placed in international database (EMBL/GenBank) with identification numbers (LR701160, LR701188, LR701436, LR701155, LR701438, LR701439, LR701440). The identity of the nucleotide sequences of region of 16S rRNA gene of Belarusian samples of Ca. P. mali ranged from 99.7 to 100.0 %, and hflB gene region ranged from 99.6 to 100.0 %.
About the Authors
T. N. Bazhydai
Institute for Fruit Growing
Belarus
Belarus
Tatsiana N. Bazhydai - Ph. D. (Biol.), Senior Researcher.
2, Kovalyov Str., 223013, а/с Samokhvalovichy, Minsk Region
E. V. Kolbanova
Institute for Fruit Growing
Belarus
Belarus
Elena V. Kolbanova - Ph. D. (Biol.), Associate Professor, Head of the Laboratory.
2, Kovalyov Str., 223013, а/с Samokhvalovichy, Minsk Region
N. V. Kukharchyk
Institute for Fruit Growing
Belarus
Belarus
Natallia V. Kukharchyk - D. Sc. (Agricult.), Professor, Head of the Department.
2, Kovalyov Str., 223013, а/с Samokhvalovichy, Minsk Region
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