Features of Arabidopsis thaliana photomorphogenesis when using LED-lighting with different spectral composition

In this work we applied the technique of growing A. thaliana Heynh plants on the surface of a hydrated cellulose film under sterile conditions on the gel environment, which was previously developed at the Institute of Biomedical Problems of the Russian Academy of Sciences. Using this technique the plant, including the root system, is fully accessible for observation and its leaves and roots are evenly illuminated. Using this technique, the features of the influence of light with a different ratio of physiologically significant spectral ranges on the morphogenesis of A. thaliana were studied. In the LED- irradiators’ spectral composition which contained all photosynthetically active radiation (PAR) wavelengths we varied the ratio of red/blue (R/B) light from 1 to 5, red/(blue-green) (R/(B + G)) from 0.7 to 4.1 and red/far red (R/FR) from 2.6 to 5.6. Luminescent lighting with R/B, R/(B + G) and R/FR ratios of 2, 1 and 11.9, respectively, was used as a control. The obtained results indicate that the growth of the aerial part and the root system, the synthesis of chlorophyll, the accumulation of dry matter of A. thaliana can be stimulated by increasing the level of red and by decreasing the level of far red light (in the presence of all other parts of PAR), reaching their certain ratio in the spectrum of LED lighting. The most effective spectral composition for the accumulation of dry and wet mass of the aerial part, the synthesizing of chlorophyll as well as for the formation of a branched root system was LED-lighting with R/B, R/(B + G) and R/FR ratios of 4, 2 and 5.6 respectively. In addition, under LED illumination of all variants, plants formed peduncles on average 5-6 days earlier and their number was greater than under luminescent one.

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