The influence of extraction parameters on the output of flavonoids from littlewale (Lithospermum officinale L.)
https://doi.org/10.29235/1029-8940-2020-65-4-402-411
Abstract
Littlewale (Lithospermum officinale L.) is a valuable medicinal plant that has been used in folk medicine since ancient times as a diuretic, laxative, analgesic, anti-cold and anti-inflammatory agent. One of the most common classes of biologically active substances of plant origin are flavonoids. The leaves of the littlewale contain isoquercitrin, a flavonoid that has a regenerating effect.
This article presents the results of a study of the influence of extraction parameters (ethanol concentration, temperature, ratio of the mass of plant materials to the volume of extractant, duration, multiplicity) on the output of flavonoids from the leaves of the littlewale. Using the orthogonal central compositional planning, a dependence is obtained that describes the influence of temperature and the duration of the extraction process on the efficiency of extracting flavonoids from the leaves of the littlewale. It was found that the maximum yield of the target components, which is more than 2 %, is achieved with the following parameters of the extraction process: extractant - 50 % ethyl alcohol, the ratio of the mass of raw materials to the volume of extractant is 1:20, temperature - 65-70 °С, duration - 35-45 minutes. It is enough to conduct a single extraction of plant materials.
About the Authors
N. Yu. Adamtsevich
Belarusian State Technological University
Belarus
Belarus
Natallia Yu. Adamtsevich - Postgraduate student.
13a, Sverdlov Str., 220006, Minsk
V. S. Boltovskiy
Belarusian State Technological University
Belarus
Belarus
Valeriy S. Boltovskiy - D. Sc. (Engineering), Assistant Professor, Professor.
13a, Sverdlov Str., 220006, Minsk
V. V. Titok
Central Botanical Garden, National Academy of Sciences of Belarus
Belarus
Belarus
Vladimir V. Titok - Corresponding Member, D. Sc. (Biol.), Assistant Professor, Director.
2v, Surganov Str., 220012, Minsk
References
1. Kurkina A. V. Flavonoids of pharmacopeia plants: monograph. Samara, Samara State Medical University, 2012. 232 p. (in Russian).
2. Tarakhovskii Yu. S., Kim Yu. A., Abdrasilov B. S., Muzafarov E. N. Flavonoids: biochemistry, biophysics, medicine. Pushchino, Synchrobook, 2013. 310 p. (in Russian).
3. Korul’kin D. Yu., Abilov J. A., Muzychkina R. A., Tolstikov G. A. Natural flavonoids. Novosibirsk, Geo Publ., 2007. 232 p. (in Russian).
4. Grotewold Е. The science of flavonoids. New York, Springer, 2006. 273 р.
5. Brown J. E., Andersen M., Markham K. R. Flavonoids: chemistry, biochemistry and applications. Boca Ratton : CRC Press, 2006. 1197 p.
6. Zverev Ya. F. Flavonoids through the eyes of a pharmacologist. Features and problems of pharmacokinetics. Obzory po klinicheskoi i lekarstvennoi terapii [Reviews of clinical and drug therapy], 2017, vol. 15, no. 2, pp. 4-11 (in Russian).
7. Makarenko O. A., Levitskii A. P. Physiological functions of flavonoids in plants. Fiziologiya i biokhimiya kul’turnykh rastenii [Physiology and biochemistry of cultivated plants], 2013, vol. 45, no. 2, pp. 100-112 (in Russian).
8. Aleksashina S. A., Makarova N. V. Comparative study of the antioxidant activity of phenolic compounds and flavonoids of tillet flowers (Tilia cordata Mill.), garden sage (Salvia officinalis L.), common melilot (Melilotus officinalis L.), currant leaves (Ribes nigrum folia), European strawberrys (Fragaria vesca L.), grapes (Vitis labrusca), growing in the Samara region. Khimiya rastitel ’nogo syr’ya [Chemistry of plant materials], 2019, no. 3, pp. 153-159 (in Russian).
9. Azarova O. V., Galaktionova L. P. Flavonoids: a mechanism of anti-inflammatory action. Khimiya rastitel’nogo syr’ya [Chemistry of plant materials], 2012, no. 4, pp. 61-78 (in Russian).
10. Rathee P., Chaudhary H., Rathee S., Rathee D., Kumar V., Kohli K. Mechanism of action of flavonoids as antiinflammatory agents : a review. Inflammation and Allergy - Drug Targets, 2009, vol. 8, no. 3, pp. 229-235. https://doi.org/10.2174/187152809788681029
11. Belitskii G. A., Kirsanov K. I., Lesovaya E. A., Yakubovskaya M. G. The mechanisms of the anticancerogenic effect of flavonoids. Uspekhi molekulyarnoy onkologii [Advances in molecular oncology], 2014, vol. 1, no. 1, pp. 56-68 (in Russian).
12. Batra P., Sharma A. K. Anti-cancer potential of flavonoids: recent trends and future perspectives. 3 Biotech, 2013, vol. 3, no. 6, pp. 439-459. https://doi.org/10.1007/s13205-013-0117-5
13. Seelinger G., Merfort I., Wolfle U., Schempp C. M. Anti-carcinogenic effects of the flavonoid luteolin. Molecules, 2008, vol. 13, no. 10, pp. 2628-2651. https://doi.org/10.3390/molecules13102628
14. Cushnie T. P., Lamb A. J. Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents, 2005, vol. 26, no. 5, pp. 343-356. https://doi.org/10.1016/j.ijantimicag.2005.09.002
15. Bhatia N., Singh A., Sharma R., Singh A., Soni V., Singh G., Bajaj J., Dhawan R., Singh B. Evaluation of burn wound healing potential of aqueous extract of Morus alba based cream in rats. Journal of Phytopharmacology, 2014, vol. 3, no. 6, pp. 378-383.
16. Bhatia N., Kaur G., Soni V., Kataria J., Dhawan R. K. Evaluation of the wound healing potential of isoquercetin-based cream on scald burn injury in rats. Burns and Trauma, 2016, vol. 4, p. 8. https://doi.org/10.1186/s41038-016-0032-1
17. Pang Y., Zhang Y., Huang L., Xu L., Wang K., Wang D., Guan L., Zhang Y., Fulai Yu., Chen Z., Xie X. Effects and mechanisms of total flavonoids from Blumea balsamifera (L.) DC. оп skin woundin rats. International Journal of Molecular Sciences, 2017, vol. 18, no. 12, pp. 2766-2778. https://doi.org/10.3390/IJMS18122766
18. Stimulation of neuroregeneration by flavonoid glycosides. Available at: www.google.com/patents/US20120087980 (accessed 07.05.2020).
19. Reddy V. P., Aryal P., Robinson S., Rafiu R., Obrenovich M., Perry G. Polyphenols in Alzheimer’s disease and in the Gut-Brain Axis. Microorganisms, 2020, vol. 8, no. 2, pp. 199-212. https://doi.org/10.3390/microorganisms8020199
20. Qi Y., Chen L., Shan S., Nie Y., Wang Y. Vitexin improves neuron apoptosis and memory impairment induced by isoflurane via regulation of miR-409 expression. Advances in Clinical and Experimental Medicine, 2020, vol. 29, no. 1, pp. 135-145. https://doi.org/10.17219/acem/104556
21. Gubanov I. A., Kiseleva K. V., Novikov V. S., Tikhomirov V. N. Illustrated identifier of plants in Central Russia. Vol. 3: Angiosperms (dicotyledonous). Moscow, Creativity of scientific publications of KMK, Institute for Technological Research, 2004. 520 p. (in Russian).
22. Sparrow medicinal (Littlewale). Available at: http://www.cbg.org.by/ecotrail/kollekciya-redkikh-i-ischezayuschikh-vidov-prirodnoy-flory-belarusi/vorobeynik (accessed 14.05.2020).
23. Baczynska B., Litynska-Zajqc M. Application of Lithospermum officinale L. in early bronze age medicine. Vegetation History and Archaeobotany, 2005, vol. 14, no. 1, pp. 77-80. https://doi.org/10.1007/s00334-004-0054-7
24. Fes’kova E. V., Leont’ev V. N., Ignatovets O. S., Adamtsevich N. Yu., Besarab A. Yu. Extraction conditions and identification of flavonoids which stimulate tissue regeneration. Trudy Belorusskogo gosudarstvennogo tekhnologicheskogo universiteta. Seriya 2: Khimicheskie tekhnologii, biotekhnologiya, geoekologiya [Proceedings of the Belarusian State Technological University. Series 2. Chemical engineering, biotechnologies, geoecology], 2019, no. 1, pp. 49-53 (in Russian).
25. Strupan E. A., Kolodyaznaya V. S., Strupan O. A. The technology of extracts from wild plant materials, widely used in the food industry and herbal medicine. Vestnik Krasnoyarskogo gosudarstvennogo agrarnogo universiteta [Bulletin of Krasnoyarsk State Agrarian University], 2012, no. 8, pp. 199-205 (in Russian).
26. Adamtsevich N. Yu., Fes’kova E. V., Boltovskii V. S. Extraction of flavonoids from littlewale (Lithospermum officinale L.) and everlasting (Helichrysum arenarium L.). Trudy Belorusskogo gosudarstvennogo tekhnologicheskogo universiteta. Seriya 2: Khimicheskie tekhnologii, biotekhnologiya, geoekologiya [Proceedings of the Belarusian State Technological University. Series 2. Chemical engineering, biotechnologies, geoecology], 2020, no. 1, pp. 93-97 (in Russian).
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