The influence of extraction parameters on the output of flavonoids from littlewale (Lithospermum officinale L.)

Abstract

Littlewale (Lithospermum officinale L.) is a valuable medicinal plant that has been used in folk medicine since ancient times as a diuretic, laxative, analgesic, anti-cold and anti-inflammatory agent. One of the most common classes of biologically active substances of plant origin are flavonoids. The leaves of the littlewale contain isoquercitrin, a flavonoid that has a regenerating effect.

This article presents the results of a study of the influence of extraction parameters (ethanol concentration, temperature, ratio of the mass of plant materials to the volume of extractant, duration, multiplicity) on the output of flavonoids from the leaves of the littlewale. Using the orthogonal central compositional planning, a dependence is obtained that describes the influence of temperature and the duration of the extraction process on the efficiency of extracting flavonoids from the leaves of the littlewale. It was found that the maximum yield of the target components, which is more than 2 %, is achieved with the following parameters of the extraction process: extractant - 50 % ethyl alcohol, the ratio of the mass of raw materials to the volume of extractant is 1:20, temperature - 65-70 °С, duration - 35-45 minutes. It is enough to conduct a single extraction of plant materials.