Influence of “Immunact-GK” and “Ecosil” preparations on the photosynthetic apparatus and oxidative status of tomato plants in conditions of small volume hydroponics
https://doi.org/10.29235/1029-8940-2020-65-3-283-291
Abstract
It was shown that the preparation “Immunact-GK” based on β-1,3-glucan exerts a multilateral positive effect on tomato plants grown under conditions of low-volume hydroponics in protected ground. In particular, it has a stimulating effect on the biosynthesis of photosynthetic pigments and the photochemical activity of PS II in chloroplast membranes, induces the accumulation of phenolic compounds, promotes the generation of ROS, which perform a signaling function and start a natural internal defense mechanisms, contributes to stabilize the level of oxidative processes in tomato leaves. It has been established that under manufacturing conditions, the preparation “Immunact-GK” provides an increase in tomato yield up to 5 kg/m2 and exceeds the efficiency of the native growth regulator “Ecosil”.
About the Authors
L. M. АbramchikBelarus
Larisa M. Abramchik - Ph. D. (Biol.), Senior Researcher, Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus.
27, Akademicheskaya Str., 220072, Minsk.
I. N. Domanskaya
Belarus
Irina N. Domanskaya - Ph. D. (Biol.), Researcher, Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus.
27, Akademicheskaya Str., 220072, Minsk.
V. N. Makarov
Belarus
Vladimir N. Makarov - Junior Researcher, Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus.
27, Akademicheskaya Str., 220072, Minsk.
E. V. Serdiuchenko
Belarus
Elena V. Serdiuchenko - Junior Researcher, Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus.
27, Akademicheskaya Str., 220072, Minsk.
T. S. Bachyshcha
Belarus
Tatsiana S. Bachyshcha - Junior Researcher, Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus.
27, Akademicheskaya Str., 220072, Minsk.
V. V. Коndratieva
Belarus
Viktoria V. Kondratyeva - Junior Researcher, Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus.
27, Akademicheskaya Str., 220072, Minsk.
Yu. N. Dovbniuk
Belarus
Yulia N. Dovbniuk - Junior Researcher, Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus.
27, Akademicheskaya Str., 220072, Minsk.
S. N. Shpilevski
Belarus
Sviatoslav N. Shpilevski - Junior Researcher, Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus.
27, Akademicheskaya Str., 220072, Minsk.
L. F. Kabashnikova
Belarus
Luidmila F. Kabashnikova - Corresponding Member, D. Sc. (Biol.), Assistant Professor, Head of the Laboratory, Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus.
27, Akademicheskaya Str., 220072, Minsk.
References
1. Gorovoi L. F., Koshevskii I. I., Teslyuk V. V., Red'ko V. V. Preparations of a new generation for plant protection. Moscow, Nauka Publ., 2010. 45 p. (in Russian).
2. Leubner-Metzger G. Functions and regulation of P-1,3-glucanases during seed germination, dormancy release and afterripening. Seed Science Research, 2003, vol. 13, no. 1, pp. 17-34. https://doi.org/10.1079/ssr2002121
3. Bagirova S. F., Dzhavakhiya V. G., D'yakov Yu. T., Ozeretskovskaya O. L., Provorov N. A., Tikhonovich I. A., Shcherbakova L. A. Fundamental phytopathology. Moscow, Krasand Publ., 2012. 508 p. (in Russian).
4. Doke N., Garas N. A., Kuc J. Effect on host hypersensitivity of suppressors released during the germination of Phytophthora infestans сystospores. Phytopathology, 1980, vol. 70, pp. 35-39. https://doi.org/10.1094/phyto-70-35
5. Rodriguez-Amaya D. B., Kimura М. HarvestPlus Handbook for carotenoid analysis. Washington, International Food Policy Research Institute, 2004. 63 p.
6. Singleton V. L., Orthofer R., Lamuela-Raventos R. M. Analysis of total phenolics and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzimology. Vol. 299. Oxidants and Antioxidants, Part A. San Diego, 1999, pp. 152-178.
7. Krause G. H., Weis E. Chlorophyll fluorescence and photosynthesis: the basics. Annual Review of Plant Physiology and Plant Molecular Biology, 1991, vol. 42, no. 1, pp. 313-349. https://doi.org/10.1146/annurev.pp.42.060191.001525
8. Merzlyak M. N. Activated oxygen and oxidative processes in plant cell membranes. Moscow, VINITI Publ., 1989. 168 p. (in Russian).
9. Heath R. L., Packer L. Photoperoxidation in isolated chloroplast. 1. Kinetics and stoichicmetry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 1968, vol. 125, no. 1, pp. 189-198. https://doi.org/10.1016/0003-9861(68)90654-1
10. LeBel C. P., Ischiropoulos H., Bondy S. C. Evaluation of the probe 2',7'-dichlorofluorescein as an indicator of reactive oxygen species formation and oxidative stress. Chemical Research in Toxicology, 1992, vol. 5, no. 2, pp. 227-231. https://doi.org/10.1021/tx00026a012
11. Mohanty J. G., Jaffe, J. S., Schulman E. S., Raible D. G. A highly sensitive fluorescent micro-assay of H2O2 release from activated human leukocytes using a dehydroxyphenoxazine derivative. Journal of Immunological Methods, 1997, vol. 202, no. 2, pp. 133-141. https://doi.org/10.1016/s0022-1759(96)00244-x
12. Karapetyan N. V., Bukhov N. G. Variable chlorophyll fluorescence as an indicator of the physiological state of plants. Fiziologiya rastenii = Plant Physiology, 1986, vol. 33, no. 5, pp. 1013-1026 (in Russian).
13. Kreslavski V. D., Allakhverdiev S. I., Los D. A., Kuznetsov V. V. Signaling role of reactive oxygen species in plants under stress. Russian Journal of Plant Physiology, 2012, vol. 59, no. 2, pp. 141-154. https://doi.org/10.1134/s1021443712020057
14. Tarchevskii I. A. Signal systems of plant cells. Moscow, Nauka Publ., 2002. 229 p. (in Russian).