Structural transitions in mixed classes of proteins
https://doi.org/10.29235/1029-8940-2019-64-3-326-337
Abstract
It was studied the features of amino acid content of protein regions of “alpha + beta” and “alpha/beta” classes, that are prone to structural transitions. The data have been obtained by the way of the comparison of different threedimensional structures of proteins with absolutely identical amino acid sequence. In this study we ignored fragments of proteins in which positions of atoms cannot be determined with the help of X-ray crystallography. Proteins of “alpha + beta” class are less stable than proteins of “alpha/beta” class, since the percent of structurally instable residues in them is higher. Most frequent type of structural transitions is the decrease of length of N-terminal and C-terminal parts of alpha helices and beta strands. Alpha helices and beta strands that can completely disappear (turn to coil) have also been found. The data of their amino acid content is important for the development of the method able to detect fragments of proteins prone to transitions from alpha helix to beta strand. Those fragments should combine characteristic features of amino acid content of both completely disappearing alpha helices and completely disappearing beta strands. The amino acid composition of alpha-helices capable to complete disappearance is significantly different from that for beta-strands capable to complete disappearance: frequencies of alanine, glutamine and glutamic acid usage are increased, frequencies of isoleucine, threonine and glycine usage are reduced.
About the Authors
V. V. Poboinev
Belarusian State Medical University
Belarus
Belarus
Victor V. Poboinev – Postgraduate student, assistant
83, Dzerzhynskii Ave., 220116, Minsk, Republic of Belarus
V. V. Khrustalev
Belarusian State Medical University
Belarus
Belarus
Vladislav V. Khrustalev – Assistant Professor, Head of the Department
83, Dzerzhynskii Ave., 220116, Minsk, Republic of Belarus
T. A. Khrustaleva
Институт физиологии НАН Беларуси
Belarus
Belarus
Tatyana A. Khrustaleva – Senior researcher
28, Akademicheskaya Str., 220072, Minsk, Republic of Belarus
A. N. Stojarov
Belarusian State Medical University
Belarus
Belarus
Aliaksandr N. Stazharau – Professor, Head of the Department
83, Dzerzhynskii Ave., 220116, Minsk, Republic of Belarus
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