Characteristic of plasmids of Bacillus pumilus isolated in Belarus
https://doi.org/10.29235/1029-8940-2019-64-3-292-301
Abstract
In current study plasmids (size from 6.2 to 8.5 kb) copied with the “rolling circle” mechanism (RCR type) of the pC194 family have been identified in environmental bacteria Bacillus pumilus. It is shown that these extrachromosomal elements are widely distributed in B. pumilus bacteria circulating on the territory of Belarus (19 strains from 41 contain RCRplasmids) and they are characterized by genetic polymorphism. The most common extrachromosomal genetic elements (7.7 kb size) are identical to the plasmid pBp15.1S from the entomopathogenic strain B. pumilus 15.1. The remaining 6 type of plasmids differ from each other and from the known extrachromosomal genetic elements of genus Bacillus. In the investigated bacteria no replicons similar to pLS20 were detected.
Supporting agencies: The study was carried out with the financial support of the Belarusian Republican Foundation for Fundamental Research (B19-110). The authors are grateful to Professor M. A. Titok, Assistant Professor V. Ye. Miamin, Assistants of the Biological Faculty of the BSU E. E. Komar (Department of Microbiology) and Yu. N. Gorovik (Department of Molecular Biology), as well as Senior researcher V. N. Akimov (IBPM RAS, Pushchino) for providing bacterial strains.
About the Authors
O. V. Evdokimova
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Belarus
Olesia V. Evdokimova – Researcher
2, Kuprevich Str., 220141, Minsk, Republic of Belarus
M. A. Chindareva
Belarusian State University
Belarus
Belarus
Maryja A. Chindareva – Student
4, Nezavisimosti Ave., 220030, Minsk, Republic of Belarus
L. N. Valentovich
Institute of Microbiology of the National Academy of Sciences of Belarus;
Belarusian State University
Belarus
Belarus
Leonid N. Valentovich – Ph. D. (Biol.), Assistant Professor, Head of the Laboratory
2, Kuprevich Str., 220141, Minsk; 4, Nezavisimosti Ave., 220030, Minsk, Republic of Belarus
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