PREBREEDING ASSESSMENT OF DWARFING GENES Rht-B1, Rht-D1, Rht8 AND Ddw1 ALLELIC COMPOSITION IN RECOMBINANT AND INTROGRESSIVE WHEAT-RYE HYBRIDES

The results of the analysis of genomic structure and allelic composition of the dwarf genes Rht-B1, Rht-D1, Rht8 and Ddw1 in 33 secondary recombinant lines (SRL) of hexaploid triticale with various types of D(A)- and D(B)- chromosome substitutions and 8 introgressive wheat lines with R(A)-, R(B)- and R(D)-chromosome substitutions are presented. It was found that in the overwhelming majority SRL genotypes contain the Rht-B1b allele in the homozygous state. Nine SRLs and all introgressive wheat lines are homozygous by the Rht-B1a allele; 4 lines are heterogeneous in the allelic composition of the Rht-B1 gene. The Rht8a allele (165 bp) was present in all the investigated SRLs, whereas the Rht8b allele (174 bp) was detected in introgressive wheat lines. Five introgressive wheat lines were heterozygous by the allelic composition of the Rht-D1 (Rht-D1a/Rht-D1b) gene. In three lines, along with the Rht-D1a/Rht-D1b genotype, plants homozygous for the RhtD1a and Rht-D1b alleles were found. No samples bearing the Ddw1 gene were identified. Based on the genotyping results, SRL of hexaploid triticale with the Rht-B1b gene ‒ promising for use in breeding for lodging resistance ‒ were selected.

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