IMPACT OF WATER DEFICIT ON THE PARAMETERS OF THE WATER EXCHANGE OF LEAVE MESOPHYLL OF MODIFIED ON THE PIP AQUAPORINS GENES ARABIDOPSIS THALIANA (L.) HEYNH. PLANTS
Abstract
The characteristics of water exchange of leaves of Arabidopsis thaliana plants, genetically modified on PIP aquaporins, at different substrate water potential. It is assumed that aquaporins PIP1 subgroup have a significant impact on the rate of stomatal water loss, the contribution of PIP2 aquaporins subgroup in a more significant flow of water into the mesophyll cells.
It is shown that the modified plants are less resistant to water stress than the wild-type plant. Increased expression of PIP aquaporins increases hygromorphic leaf structure, less resistant to water stress were overexpressor pip2; 2-23. Under the conditions of water stress in plants knockout pip2; 1-2 increases resistance to mesophyll tissue dehydration.
About the Authors
E. S. ZubeiBelarus
Researcher
27, Akademicheskaya Str., 220072
V. G. Reutskiy
Belarus
D. Sc. (Biol.), Professor
27, Akademicheskaya Str., 220072
References
1. Chaumont F., Tyerman S. D. Aquaporins : highly regulated channels controlling plant water relations. Plant Physiology, 2014, vol. 164, iss. 4, pp. 1600–1618. DOI: https://doi.org/10.1104/pp.113.233791
2. Johanson U., Karlsson M., Johansson I. Gustavsson S., Sjövall S., Fraysse L., Weig A. R., Kjellbom P. The complete set of genes encoding major intrinsic proteins in Arabidopsis provides a framework for a new nomenclature for major intrinsic proteins in plants. Plant Physiology, 2001, vol. 126, no. 4, pp. 1358–1369.
3. Barrieu F., Morillon R., Chrispeels M. J. Modulation of aquaporin gene expression in Arabidopsis leads to altered membrane water permeability. Molecular Biology and Physiology of Water and Solute Transport. New York, 2000, pp. 255–259.
4. Prado K., Boursiac Y., Tournaire-Roux C., Postaire O., Da Ines O., Schäffner A. R., Hem S., Santoni V., Maurel C. Regulation of Arabidopsis leaf hydraulics involves light-dependent phosphorylation of aquaporins in veins. The Plant Cell, 2013, vol. 25, iss. 3, pp. 1029–1039. DOI: 10.1105/tpc.112.108456
5. Postaire O., Tournaire-Roux C., Grondin A., Boursiac Y., Morillon R., Schäffner A. R., Maurel C. PIP1 aquaporin contributes to hydrostatic pressure-induced water transport in both the root and rosette of Arabidopsis. Plant Physiology, 2010, vol. 152, no. 3, pp. 1418–1430. DOI: 10.1104/pp.109.145326
6. Lee S. H., Chung G. C., Jang J. Y., Ahn S. J., Zwiazek J. J. Overexpression of PIP2;5 aquaporin alleviates effects of low root temperature on cell hydraulic conductivity and growth in Arabidopsis. Plant Physiology, 2012, vol. 159, iss. 1, pp. 479–488.
7. Aharon R., Shahak Y., Wininger S. Bendov R., Kapulnik Y., Galili G. Overexpression of a plasma membrane aquaporin in transgenic tobacco improves plant vigor under favorable growth conditions but not under drought or salt stress. Plant Cell, 2003, vol. 15, pp. 439–447.
8. Siefritz F., Tyree M. T., Lovisolo C., Schubert A., Kaldenhoff R. PIP1 plasma membrane aquaporins in tobacco: from cellular effects to functions in plants. Plant Cell, 2002, vol. 14, pp. 869–876. DOI: 10.1074/mcp.M113.028241
9. Grinyuk E. V., Fomina E. K., Yakimtsova L. B., Krul L. P. New film-forming biotechnical preparations of agricultural purpose based on chemically cross-linked functionalized polyacrylamides. Sviridovskie chtenija = Sviridov’s readings, Belarusian State Institute, Research Institute of Physical and Chemical Problems, Faculty of Chemistry, Chair of Inorganic Chemistry, in Ivashkevich O. A. (ed.), et al. Minsk, 2012, iss. 8, pp. 194–201 (in Russian).
10. Zubej E. S., Rodionov P. A., Teljuk N. A., Reuckij V. G. Method of electronic monitoring of water exchange of leaf assimilation tissue. Botanika: (issledovanija) [Botanica: (research)], National Academy of Sciences of Belarus, Department of Biological Sciences, Institute of Experimental Botany of. V. F. Kuprevich National Academy of Sciences of Belarus, Belarusian Botanical Society, Belarusian Society of Plant Physiologists, in Laman N. A. (ed.), et al. Minsk, 2016, iss. 45. pp. 299–308 (in Russian).
11. Reuckij V. G., Zubej E. S., Skuratovich T. A., Rodionov P. A. Features of water exchange in the apoplast-protoplast system of mesophyll leaf cells as a stress-resistance factor of plants. Botanika: (issledovanija) [Botanica: (research)], National Academy of Sciences of Belarus, Department of Biological Sciences, Institute of Experimental Botany of. V. F. Kuprevich National Academy of Sciences of Belarus, Belarusian Botanical Society, Belarusian Society of Plant Physiologists, in Laman N. A. (ed.), et al. Minsk, 2010, iss. 39, pp. 375–388 (in Russian).
12. Glanc S. Medico-biological statistics. Moscow, Practica Publ., 1998. 459 p. (in Russian).
13. Maurel C., Verdoucq L., Luu D., Santoni V. Aquaporins: Membrane Channels with Multiple Integrated Functions. Annual Review of Plant Biology, 2008, vol. 59, pp. 595–624. DOI: 10.1146/annurev.arplant.59.032607.092734
14. Aroca R., Ferrante A., Vernier P., Chrispeels M. J. Drought, abscisic acid and transpiration rate effects on the regulation of PIP aquaporin gene expression and abundance in Phaseolus vulgaris plants. Annals of Botany, 2006, vol. 98. iss. 6, рр. 1301–1310. DOI:10.1093/aob/mcl219
15. Teljuk N. A., Reuckij V. G., Rodionov P. A. Water regime of assimilating leaf tissue of plants of different ecological groups. Problemy jeksperimental’noj botaniki : k 100-letiju so dnja rozhdenija V. F. Kuprevicha [Problems of experimental botany: to the 100th anniversary of the birth of V. F. Kuprevich], Academy of Sciences of Belarus, Institute of Experimental Botany named after V. F. Kuprevich, in Parfenov V. I. (ed.). Minsk, 1997, pp. 436–448 (in Russian).