INFLUENCE OF 5-AMINOLEVULINIC ACID ON THE PRODUCTIVITY AND PIGMENT COMPOSITION OF ALGAE HAEMATOCOCCUS PLUVIALIS

Influence of exogenous 5-aminolevulinic acid (ALA) on the algae Haematococcus pluvialis productivity – dry weight, number and size of cells as well as proteins and photosynthetic pigment content has been studied. Growing of algae cells in Rudic’s medium with supplementation of ALA (0.5; 5; 10; 20 and 30 mg/l) stimulated accumulation of cell dry weight during active algae growth as compared with control cells as well as with initial algae culture. So, for two days of algae incubation with ALA 10 mg/l the dry weight increased by an average of 31 % in relation to initial culture and by 17 % in relation to control. A dose-dependent increase in content of cell number by an average of 26 % and decrease of cell diameter by 15 % was registered in “ALA” variants as compared with these characteristics in control. After 7 days of incubation with ALA content of the photosynthetic pigments and proteins increased. So, content of chlorophylls a, b and β-carotene per g of dry matter exceeded the control values by 37, 37 and 58 %, respectively. For xanthophylls such as neoxanthin, violaxanthin and lutein – by 31, 30 and 47 %, respectively. While the protein content on average increased by 20–73 %. After 12 days of incubation, the effectiveness of ALA decreased while maintaining the above trend. The results are discussed from the position of using exogenous ALA in the synthesis of endogenous cytokinins that stimulated the growth and development of algal cells, as well as the formation of the pigmentary apparatus of photosynthesis. 

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