REPRESENTATIVENNESS OF DNA-MARKERS OF LATE BLIGHT AND PVY-RESISTANCE GENES IN ACCESSIONS OF WILD ALLOTETRAPLOID POTATO SPECIES SOLANUM STOLONIFERUM

Wild allotetraploid potato species from Mexico Solanum stoloniferum Schltdl. (series Longipedicellata) is considered to be a source of the range of valuable for breeding genes. Nevertheless, natural reproductive barriers between S. stoloniferum and cultivated potatoes considerably hamper introgression of genes of this species into breeding material. We were the first who demonstrated earlier the possibility of production of diploid hybrids in crosses between S. stoloniferum and S. tuberosum diploid lines that can substantially simplify this process. However, it is not clear which of the wild species genes situated on different genomes may be transferred to diploid interspecific hybrids. The purpose of the study was to select wild species genotypes with the widest range of DNA-markers of LB- and PVY-resistance genes. They will be used in crosses with S. tuberosum diploid lines to investigate the transfer of the markers along with A (or B) genome of the wild species to diploid hybrids as well as in application of other methods of involvement of S. stoloniferum into breeding. As a result of study 26 accessions of wild allotetraploid species S. stoloniferum, the PI 205522 had been selected carrying markers of Rpi-sto1, Ry-sto and Ry-fsto genes. Lack of segregation on the markers in offspring from self-pollination of PI 205522 indicated about homozygous state of these genes. Sequence of the part of the 1521/518 marker in PI 205522 had shown its homology (99 %) with known sequences of genes Rpi-sto1 of S. stoloniferum and Rpi-blb1 of S. bulbocastanum of the accessions of these species having high LB-resistance.

potato, Solanum stoloniferum, DNA-markers, resistance genes, PVY (potato virus Y), LB (late blight)

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