RADIATION INJURY SPERMATOGENIC CELLS AND SPERMATOZOA RATS WISTAR AFTER EXTERNAL EXPOSURE

It was studied the effect of external radiation (1.0 Gy) on the reproductive system of male rats. It is established that in the initial period after irradiation, acceleration of the process of spermatogenesis is observed, which is confirmed by an increase in the number of spermatogonia, preleptotene spermatocytes, and spermatocytes of the first order and round spermatids. Simultaneously, a decrease in the number of epididymal spermatozoa and their viability, a significant death of mature sex cells by apoptosis and necrosis. The activity of GDD and acrosin in epididymal spermatozoa decreases on the 3rd day after irradiation, while in the long-term period the activity of GFDG does not differ from control at an increased level of acrosin (almost 2.8 times). The deterioration of quantitative and qualitative characteristics of epididymal spermatozoa in irradiated animals, apparently, will lead to a decrease in the fertility of experimental animals.

external exposure at dose 1.0 Gy, male rats reproductive systems, spermatogenesis, spermatozoa, viability, DNA fragmentation index (index DFI), apoptosis, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), acrosyn, fructose

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