MOLECULAR CHARACTERIZATION OF BLUEBERRY RED RINGSPOT VIRUS ISOLATE
Abstract
The fragment of BRRV isolate from Belarus (BRRV-BY1) was sequenced for the first time and nucleotide sequence of this isolate was deposited in GenBank with the accession No. LN998983. Sequence analysis revealed that the Belarusian isolate shared 94.1–99.3 % identity with 18 isolates of the virus available in GenBank. BRRV isolate from Belarus was mostly closely related to those from the USA (accession Nos JF917083 and JF917082) as they shared 99.3 % nucleotide identity. Phylogenetic analysis showed that clustering of investigated isolates didn’t depend on their geographical origin.
About the Authors
T. N. Bazhydai
Institute for Fruit Growing
Belarus
Scientific researcher
Belarus
Scientific researcher
N. V. Kukharchyk
Institute for Fruit Growing
Belarus
D. Sc. (Agricult.), Assistant Professor, Head of the Department
Belarus
D. Sc. (Agricult.), Assistant Professor, Head of the Department
References
1. Glasheen B. M., Polashock J. J., Lawrence D. M., Gillett J. M., Ramsdell D. C., Vorsa N., Hillman B. I. Cloning, sequ- encing and promoter identification of Blueberry red ringspot virus, a member of the family Caulimoviridae with similarities to the “Soybean chlorotic mottle-like” genus. Archives of Virology, 2002, vol. 147, pp. 2169–2186. doi: 10.1007/s00705-002-0866-7.
2. Kalinowska E., Paduch-Cichal E., Chodorska M. Molecular characterization of polish blueberry red ringspot virus isolate. Virus Genes, 2012, vol. 44, pp. 309–311. doi: 10.1007/s11262-011-0679-4.
3. Martin R. R., Tzanetakis I. E., Caruso F. L., Polashock J. J. Emerging and reemerging virus diseases of blueberry and cranberry. Acta Horticulturae, 2009, vol. 810, pp. 299–304. doi: 10.17660/ActaHortic.2009.810.38.
4. Martin R. R., Polashock J. J., Tzanetakis I. E. New and emerging viruses of blueberry and cranberry. Viruses, 2012, vol. 4, no. 11, pp. 2831–2852. doi: 10.3390/v4112831.
5. Polashock J. J., Ehlenfeldt M. K., Crouch J. A. Molecular detection and discrimination of Blueberry red ringspot virus strains causing disease in cultivated blueberry and cranberry. Plant Disease, 2009, vol. 93, no. 7, pp. 727–733. doi: 10.1094/ PDIS-93-7-0727.
6. AFAO/IPGRI Technical guidelines for the safe movement of small fruit germplasm, in Diekmann M., Frison E., Putter T. (ed.), in collaboration with the Small Fruit Virus Working Group of the International Society for Horticultural Science. Rome, Food and Agriculture of the United Nations, International Plant Genetic Resources Institute, 1994, 124 p.
7. Cline W. O., Ballington J. R., Polashock J. J. Blueberry red ringspot observations and findings in North Carolina. Acta Horticulturae, 2009, vol. 810, pp. 305–312. doi: 10.17660/ActaHortic.2009.810.39.
8. Paduch-Cichal E., Kalinowska E., Chodorska M., Sala-Rejczak K., Nowak B. Detection and identification of viruses of highbush blueberry and cranberry using serological ELISA test and PCR technique. Acta Scientiarum Polonorum, Hortorum Cultus, 2011, vol. 10, no. 4, pp. 201–215.
9. Kalinowska E., Paduch-Cichal E., Chodorska M., Sala-Rejczak K. First report of Blueberry red ringspot virus in high-bush blueberry in Poland. Journal of Plant Pathology, 2011, vol. 93, no. 4, pp. 73. doi: http://dx.doi.org/10.4454/jpp.v93i4.2381.
10. Pribylova J., Spak J., Kubelkova D., Petrzik K. First report of Blueberry red ringspot virus in highbush blueberry in the Czech Republic. Plant Disease, 2010, vol. 94, no. 8, pp. 1071. doi: http://dx.doi.org/10.1094/PDIS-94-8-1071B.
11. Plesko I. M., Marn M. V., Koron D. Detection of Blueberry red ringspot virus in highbush blueberry cv. ‘Coville’ in Slovenia. Julius-Kuhn-Archives, 2010, vol. 427, pp. 204–205.
12. Certification schemes. Pathogen-tested material of Vaccinium spp.: EPPO Standards PM 4/18 (1). Bulletin OEPP/ EPPO, 1997, vol. 27, pp. 195–204.
Review
Views: 489
Email this article 
