MORPHOLOGICAL ANALYSIS OF POLLEN OF THE GENUS PERSICA MILL. SPECIES

In the conditions of the Forest steppe zone of Ukraine the adverse meteorological phenomena is observed, as a result it exerts the dominating impact on peach's pollen development. Therefore the main task of current research was the generative features of genus Persica. The purpose – to study pollen micromorphological features of a surface the sort Persica Mill. representatives in the conditions of Forest steppe zone to show differences which could be used in identification of a genus. Objects of research – species of P. davidiana Carr. and P. vulgaris Mill. collections of the National botanical garden of M. M. Gryshko NAS of Ukraine. To carry out our research we used previously dried pollen. At research of morphological parameters we used scanning electronic microscopes of REMMA-102 of JSC SELMI (Sumy, Ukraine) and GSM-6700F (JEOL, Japan). For measurements of pollen grains we used the Axio Vision 40 V 4.8.2.0 (Carl Zeiss) licensed program. Sculptural elements of pollen are located meridionaly and in parallel, usually faltering, short and extended walls alternate with twisting fillets. Results of measurements of length of a polar axis and equatorial diameter showed that pollen grains of the studied species are characterized as big and vary in amount from 47.89 to 64.27 mkm on a polar axis and 25.60 to 34.74 mkm from the equatorial diameter. The average index of a form is in the range from 1.75 to 1.99 mkm which is smaller for P. davidiana (1.75–1.80). Comparative and morphological analysis of pollen of two peach species in conditions of the Forest steppe zone of Ukraine showed that qualitative signs of difference of pollen grains P. davidiana and P. vulgaris. First of all there are lack of perforation, a form and sculptural elements, and also quantitative: length of a polar axis and equatorial diameter. The statistical analysis of the received values and the allocated signs of studied peach genotypes allowed us to mark out some features which would be helpful at identification of pollen grains of different species.

peach, pollen, species, sculpture, exine, perforation

1. Golubinskiy, I. E. (1974), Biologiya prorastaniya pyiltsyi [Pollen germination biology], Naukova dumka, UA.

2. Motyleva, S. M., Dzhigadlo, Ye. N., Kuznetsov, M. N. and Mertvishcheva, M. E. (2011), “Features of the microstructure of pollen grains of some members of the genus Prunus L.”, Dostizheniya i perspektivy razvitiya selektsii, vozdelyvaniya i ispolzovaniya plodovykh kultur [Achievements and prospects of breeding, cultivation and utilization of horticultural crops], Yalta, UA, rr. 44-46.

3. Sokolova, E. A. (2000), “The value of the anatomical features of the systematics of the subfamily Prunoideae (Rosa- ceae)”, Ph. D. Theses, Sankt-Peterburg, RU.

4. Samvelyan, G. E. and Bashkiryan, A. I. (1980), “About the pollen of different quality grades Rersica vulgaris Mill in Armenian SSR”, Biologicheskiy zhurnal Armenii, no. 33 (7), pp. 703-708.

5. Karupu, V. Ya. (1984), Elektronnaya mikroskopiya [Electron microscopy], Nauka, Kyiv, UA.

6. Uikli, B. (1975), Elektronnaya mikroskopiya dlya nachinayuschih [A beginner᾿s handbook in biological electron microscory], Mir, Moscow, RU.

7. Kupriyanova, L. A. and Aleshina, L. A. (1978), Pyiltsa dvudolnyih rasteniy Evropeyskoy chasti SSSR [Pollen dicotyledonous plants of the European part of the USSR], Nauka, Leningrad, RU.

8. Urbakh, V. Yu. (1963), Matematicheskaya statistika dlya biologov i medikov [Mathematical statistics for biologists and physicians], Izdatel᾿stvo Akademii nauk SSSR, Moscow, RU.

9. Fedorov, A. A. and Artyushenko, Z. T. (1975), Atlas po opisatelnoy morfologii vyisshih rasteniy. Tsvetok [Atlas of descriptive morphology of higher plants. Flower], Nauka, Leningrad, RU.

10. Tokarev, P.I. (2002), Morfologiya i ultrastruktura pyiltsevyih zeren [Morphology and ultrastructure of pollen], KMK, Moscow, RU.